LOCUS       BBa_K1321367            1486 bp    DNA     linear       10-May-2024
DEFINITION  sfGFP + linker-CBDcipA-linker with T7 promoter
ACCESSION   BBa_K1321367
VERSION     BBa_K1321367.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     CDS             1..43
                     /label=BBa_K1321338
     misc_feature    50..52
                     /label=start
     misc_feature    44..58
                     /label=BBa_K1319106
     CDS             59..769
                     /label=sfGFP
     CDS             59..769
                     /label=BBa_K1321337
     misc_feature    770..775
                     /label=Scar 25
     misc_feature    770..775
                     /label=BBa_B0105
     CDS             776..901
                     /label=Endogenous Linker (N-term)
     CDS             902..1378
                     /label=CBDcipA
     CDS             1379..1486
                     /label=Endogenous Linker (C-term)
     CDS             776..1486
                     /label=BBa_K1321014
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggccggccg
       61 taaaggcgaa gagctgttca ctggtgtcgt ccctattctg gtggaactgg atggtgatgt
      121 caacggtcat aagttttccg tgcgtggcga gggtgaaggt gacgcaacta atggtaaact
      181 gacgctgaag ttcatctgta ctactggtaa actgccggta ccttggccga ctctggtaac
      241 gacgctgact tatggtgttc agtgctttgc tcgttatccg gaccatatga agcagcatga
      301 cttcttcaag tccgccatgc cggaaggcta tgtgcaggaa cgcacgattt cctttaagga
      361 tgacggcacg tacaaaacgc gtgcggaagt gaaatttgaa ggcgataccc tggtaaaccg
      421 cattgagctg aaaggcattg actttaaaga agacggcaat atcctgggcc ataagctgga
      481 atacaatttt aacagccaca atgtttacat caccgccgat aaacaaaaaa atggcattaa
      541 agcgaatttt aaaattcgcc acaacgtgga ggatggcagc gtgcagctgg ctgatcacta
      601 ccagcaaaac actccaatcg gtgatggtcc tgttctgctg ccagacaatc actatctgag
      661 cacgcaaagc gttctgtcta aagatccgaa cgagaaacgc gatcatatgg ttctgctgga
      721 gttcgtaacc gcagcgggca tcacgcatgg tatggatgaa ctgtacaaaa ccggcaatgc
      781 tacgccaact aagggtgcaa ccccgaccaa cacagcaacg cctacaaaaa gcgctacagc
      841 aacacctaca agaccgtcag ttcctacaaa cacaccgact aacacaccgg caaatacacc
      901 tgtttcaggc aacttgaagg tcgaattcta taactcaaat ccgagtgata caactaacag
      961 tattaatccg cagtttaaag taacaaatac aggatcaagt gcaattgatc tttcaaagct
     1021 tacattgaga tactattaca ccgttgatgg ccagaaggac cagactttct ggtgtgacca
     1081 tgcagctatc ataggtagca acggctcata caacggcatc acatcaaatg taaaaggaac
     1141 attcgtaaag atgagctcaa gcacaaataa cgcagacaca tacctcgaaa taagtttcac
     1201 aggtggcact ttggaacctg gtgctcatgt acagatacag ggtaggtttg cgaaaaatga
     1261 ctggagtaat tatacacagt caaatgatta ctcatttaag tcagcatcac agttcgtaga
     1321 atgggatcag gttacagcat atttgaatgg agtacttgta tggggtaaag aaccaggagg
     1381 atcagtagtt ccgtcaacac agccggtaac aaccccaccg gcaacaacca agccgccagc
     1441 aacaaccaaa ccaccggcta ccacgattcc tccatcagac gatccg
//