LOCUS       BBa_K1321371            1663 bp    DNA     linear       17-May-2024
DEFINITION  Linker-CBDcipA-Linker + sfGFP with LacI promoter
ACCESSION   BBa_K1321371
VERSION     BBa_K1321371.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    227..229
                     /label=start
     misc_feature    221..235
                     /label=BBa_K1319106
     CDS             236..361
                     /label=Endogenous Linker (N-term)
     CDS             362..838
                     /label=CBDcipA
     CDS             839..946
                     /label=Endogenous Linker (C-term)
     CDS             236..946
                     /label=BBa_K1321014
     misc_feature    947..952
                     /label=Scar 25
     misc_feature    947..952
                     /label=BBa_B0105
     CDS             953..1663
                     /label=sfGFP
     CDS             953..1663
                     /label=BBa_K1321337
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ccggcaatgc
      241 tacgccaact aagggtgcaa ccccgaccaa cacagcaacg cctacaaaaa gcgctacagc
      301 aacacctaca agaccgtcag ttcctacaaa cacaccgact aacacaccgg caaatacacc
      361 tgtttcaggc aacttgaagg tcgaattcta taactcaaat ccgagtgata caactaacag
      421 tattaatccg cagtttaaag taacaaatac aggatcaagt gcaattgatc tttcaaagct
      481 tacattgaga tactattaca ccgttgatgg ccagaaggac cagactttct ggtgtgacca
      541 tgcagctatc ataggtagca acggctcata caacggcatc acatcaaatg taaaaggaac
      601 attcgtaaag atgagctcaa gcacaaataa cgcagacaca tacctcgaaa taagtttcac
      661 aggtggcact ttggaacctg gtgctcatgt acagatacag ggtaggtttg cgaaaaatga
      721 ctggagtaat tatacacagt caaatgatta ctcatttaag tcagcatcac agttcgtaga
      781 atgggatcag gttacagcat atttgaatgg agtacttgta tggggtaaag aaccaggagg
      841 atcagtagtt ccgtcaacac agccggtaac aaccccaccg gcaacaacca agccgccagc
      901 aacaaccaaa ccaccggcta ccacgattcc tccatcagac gatccgaccg gccgtaaagg
      961 cgaagagctg ttcactggtg tcgtccctat tctggtggaa ctggatggtg atgtcaacgg
     1021 tcataagttt tccgtgcgtg gcgagggtga aggtgacgca actaatggta aactgacgct
     1081 gaagttcatc tgtactactg gtaaactgcc ggtaccttgg ccgactctgg taacgacgct
     1141 gacttatggt gttcagtgct ttgctcgtta tccggaccat atgaagcagc atgacttctt
     1201 caagtccgcc atgccggaag gctatgtgca ggaacgcacg atttccttta aggatgacgg
     1261 cacgtacaaa acgcgtgcgg aagtgaaatt tgaaggcgat accctggtaa accgcattga
     1321 gctgaaaggc attgacttta aagaagacgg caatatcctg ggccataagc tggaatacaa
     1381 ttttaacagc cacaatgttt acatcaccgc cgataaacaa aaaaatggca ttaaagcgaa
     1441 ttttaaaatt cgccacaacg tggaggatgg cagcgtgcag ctggctgatc actaccagca
     1501 aaacactcca atcggtgatg gtcctgttct gctgccagac aatcactatc tgagcacgca
     1561 aagcgttctg tctaaagatc cgaacgagaa acgcgatcat atggttctgc tggagttcgt
     1621 aaccgcagcg ggcatcacgc atggtatgga tgaactgtac aaa
//