LOCUS BBa_K1322001 3909 bp DNA linear 27-Dec-2024 DEFINITION Self-Regulating Oscillating Fluorescent System ACCESSION BBa_K1322001 VERSION BBa_K1322001.1 FEATURES Location/Qualifiers RBS 44..55 /label=RBS RBS 1068..1079 /label=RBS RBS 2127..2138 /label=RBS RBS 2996..3007 /label=RBS misc_feature 1836..1868 /label=LVA misc_feature 2724..2756 /label=LVA misc_feature 3734..3766 /label=LVA CDS 2145..2787 /label=AHL Autoinducer Synthetase CDS 1086..1899 /label=Autoinducer Inactivation Enzyme A CDS 3014..3772 /label=GFP promoter 1..35 /label=Constitutive Promoter CDS 62..842 /label=LuxR Repressor/Activator promoter 2933..2987 /label=LuxR/HSL Regulated Promoter promoter 2045..2118 /label=pLux/Lac Hybrid Promoter promoter 988..1059 /label=pLux/Tet Hybrid Promoter misc_feature 851..930 /label=T1 Terminator misc_feature 1908..1987 /label=T1 Terminator misc_feature 2796..2875 /label=T1 Terminator misc_feature 3781..3860 /label=T1 Terminator misc_feature 939..979 /label=TE Terminator misc_feature 1996..2036 /label=TE Terminator misc_feature 2884..2924 /label=TE Terminator misc_feature 3869..3909 /label=TE Terminator ORIGIN 1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta 61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag 121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata 181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga 241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc 301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa 361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac 421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca 481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt 541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa 601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc 661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa 721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac 781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc 841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 961 gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa 1021 tggtttgtta tagtcgaata tccctatcag tgatagagat actagagaaa gaggagaaat 1081 actagatgac agtaaagaag ctttatttcg tcccagcagg tcgttgtatg ttggatcatt 1141 cgtctgttaa tagtacatta acaccaggag aattattaga cttacctgtt tggtgttatc 1201 ttttggagac tgaagaagga cctattttag tagatacagg tatgccagaa agtgcagtta 1261 ataatgaagg tctttttaac ggtacatttg tcgaagggca ggttttaccg aaaatgactg 1321 aagaagatag aatcgtgaat attttaaaac gggttggtta tgagccggaa gaccttcttt 1381 atattattag ttctcacttg cattttgatc atgcaggagg aaatggcgct tttataaata 1441 caccaatcat tgtacagcgt gctgaatatg aggcggcgca gcatagcgaa gaatatttga 1501 aagaatgtat attgccgaat ttaaactaca aaatcattga aggtgattat gaagtcgtac 1561 caggagttca attattgcat acaccaggcc atactccagg gcatcaatcg ctattaattg 1621 agacagaaaa atccggtcct gtattattaa cgattgatgc atcgtatacg aaagagaatt 1681 ttgaaaatga agtgccattt gcgggatttg attcagaatt agctttatct tcaattaaac 1741 gtttaaaaga agtggtgatg aaagagaagc cgattgtttt ctttggacat gatatagagc 1801 aggaaagggg atgtaaagtg ttccctgaat atatagctgc aaacgacgaa aactacgctt 1861 tagtagctta ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat 1921 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 1981 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 2041 agagacctgt aggatcgtac aggtttactt gtgagcggat aacaatatag tgtgtggaat 2101 tgtgagcgga taacaattta ctagagaaag aggagaaata ctagatgact ataatgataa 2161 aaaaatcgga ttttttggca attccatcgg aggagtataa aggtattcta agtcttcgtt 2221 atcaagtgtt taagcaaaga cttgagtggg acttagttgt agaaaataac cttgaatcag 2281 atgagtatga taactcaaat gcagaatata tttatgcttg tgatgatact gaaaatgtaa 2341 gtggatgctg gcgtttatta cctacaacag gtgattatat gctgaaaagt gtttttcctg 2401 aattgcttgg tcaacagagt gctcccaaag atcctaatat agtcgaatta agtcgttttg 2461 ctgtaggtaa aaatagctca aagataaata actctgctag tgaaattaca atgaaactat 2521 ttgaagctat atataaacac gctgttagtc aaggtattac agaatatgta acagtaacat 2581 caacagcaat agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca 2641 aagaaattca tgtattaggt gatactaaat cggttgtatt gtctatgcct attaatgaac 2701 agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat 2761 aactctgata gtgctagtgt agacctctac tagagccagg catcaaataa aacgaaaggc 2821 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 2881 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agacctgtag 2941 gatcgtacag gtttacgcaa gaaaatggtt tgttatagtc gaataaatac tagagaaaga 3001 ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt 3061 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga 3121 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc 3181 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga 3241 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag 3301 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg 3361 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat 3421 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa 3481 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt 3541 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc 3601 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga 3661 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact 3721 atacaaaagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat aatactagag 3781 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 3841 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 3901 gcgtttata //