LOCUS       BBa_K1323003            2257 bp    DNA     linear       25-Nov-2024
DEFINITION  oriT-Nes: expression sequence for Staphylococcus conjugation
ACCESSION   BBa_K1323003
VERSION     BBa_K1323003.1  
FEATURES             Location/Qualifiers
     promoter        81..126
                     /label=Putative Promoter
     misc_feature    55..66
                     /label=oriT
     RBS             153..158
                     /label=Putative RBS
     CDS             166..2163
                     /label=Nickase Coding Sequence
     stem_loop       2176..2205
                     /label=Rho-independant Terminator
ORIGIN
        1 ccttttcgtt tgacagattc aagttttacc gttataatct agttaaatcc cgtaagggcg
       61 cacttatgcg aacgttccgt tcgcgtgcta aattcgtgaa attaaaaatt aatttttaat
      121 ttatttcgcc ttcgctcaat atttgttttt taaggagatt ttattatggc aatgtaccat
      181 tttcaaaata agtttgtatc aaaagcaaat ggacaaagtg caactgcaaa aagtgcctat
      241 aatagcgcta gtaggattaa agattttaag gaaaacgaat ttaaagatta ctcaaataag
      301 caatgtgatt atagtgaaat tttattacct aataatgctg atgataagtt taaagataga
      361 gaatatttgt ggaataaagt tcatgatgtt gagaacagaa aaaatagtca agttgcacga
      421 gaaattataa ttggtttgcc gaatgaattt gatccaaata gtaatattga attagcaaaa
      481 gaatttgctg aatccttatc taatgaagga atgattgttg atctaaatat tcataaaata
      541 aatgaagaaa atcctcacgc acatttatta tgtacattac gtggtttaga taaaaataat
      601 gaatttgaac ctaagcgaaa gggcaatgat tatattagag attggaatac aaaagaaaaa
      661 cataatgaat ggcgaaaacg gtgggaaaat gttcaaaata aacacctaga aaagaatggt
      721 ttttctgtta gagtttctgc tgattcatat aaaaatcaaa atatagattt agagccaaca
      781 aaaaaagaag gttggaaagc acgaaaattt gaagatgaaa caggaaaaaa atcttcaata
      841 tcgaaacata atgaaagtat taaaaagaga aatcagcaaa aaattgataa gatgtttaat
      901 gaagtagatg atatgaaatc tcacaaatta aatgctttta gttatatgaa taaatcagat
      961 tctactacat taaaaaatat ggccaaagat ttaaaaattt atgtaacacc tataaatatg
     1021 tataaggaaa atgaaagact atatgattta aaacaaaaaa cttcattaat cactgatgat
     1081 gaagatagat taaataaaat tgaggatata gaggatagac agaaaaaact tgaatctata
     1141 aatgaggtat ttgaaaaaca agctggaatt ttctttgata aaaattatcc cgatcaatca
     1201 ttaaactata gtgatgatga aaaaatcttt atcacacgca ctatattaaa tgatagagat
     1261 gttttaccag ctaataatga actagaggat atagttaaag agaaacgcat aaaagaggct
     1321 caaataagcc ttaatacagt tttaggtaac agagatataa gtttagaatc tattgaaaaa
     1381 gaatcgaatt tctttgccga taaactttca aatattctcg aaaaaaataa cttgtctttt
     1441 gatgatgtgt tagagaataa acatgaaggt atggaagata gtttgaaaat agattattat
     1501 actaacaaat tagaagtttt tagaaatgct gaaaatatac tagaagatta ttatgatgta
     1561 caaataaagg aattatttac agatgatgaa gattataaag cttttaatga agtaactgac
     1621 attaaagaaa aacaacaatt aattgatttt aaaacttatc atggtacaga aaacacaatt
     1681 gaaatgttag aaacaggtaa ttttattcct aaatacagtg atgaagatag aaaatatata
     1741 actgaacaag ttaagttgtt acaagaaaaa gaatttaaac ctaataaaaa tcagcatgat
     1801 aaatttgtat ttggggctat tcaaaagaaa ttattgagtg aatatgactt tgattatagt
     1861 gataacaatg atttaaaaca tttatatcaa gaatccaatg aagtagggga cgaaataagc
     1921 aaagataaca ttgaagagtt ttatgaaggt aatgatattc tagtagataa agaaacttac
     1981 aataattaca atagaaaaca acaagcctat ggtttaataa atgctggact agattcaatg
     2041 atatttaatt ttaatgagat atttagagaa cgtatgccga agtatataaa tcatcagtac
     2101 aaaggtaaga atcacagtaa acagcgccat gagttgaaaa ataaaagagg tatgcactta
     2161 taaatagtat tgtataaaaa aagaaggcgt atatgccttc tttttatata tcaatttctt
     2221 cttcttgttt gttttcaatt tctttattct gttcatc
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