LOCUS BBa_K1323017 5945 bp DNA linear 28-Jun-2024 DEFINITION E. coli - Staphylococcus Shuttle Vector ACCESSION BBa_K1323017 VERSION BBa_K1323017.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Biobrick suffix stem_loop 22..93 /label=E. coli his operon terminator stem_loop 30..64 /label=Stem loop primer_bind 157..176 /label=Verification reverse (VR) primer binding site misc_feature 178..191 /label=Scar: BamHI sites used to insert parts misc_feature 192..2124 /label=oriV (Staphylococcal ORI from pSK41) CDS 2172..2903 /label=Erythromycin resistance misc_feature 2978..2983 /label=Scar: BglII site used to insert parts misc_feature 3066..3680 /label=rep (pMB1) misc_feature 3081..3081 /label=ORI stem_loop 3847..3952 /label=T0 Terminator CDS 3966..4625 /label=Chloramphenicol resistance primer_bind 4738..4757 /label=Verification forward (VF2) primer binding site stem_loop 4814..4817 /label=end of terminator stem_loop 4827..4846 /label=Stem loop misc_feature 4855..4876 /label=Biobrick prefix misc_feature 4877..5945 /label=BBa_J04450 ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt 61 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct 121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggga 181 tccatggatc caagctttaa ttactttaac cattgctacc ttcgttgaag gtgcctgatc 241 tgtaattacc ttttgaggtt taccaaattg tttaatgaga cgtttgataa acgcatatgc 301 tgaatgatta tctcgttgct tacgcaacca aatatctaat gtatgtccct ctgcatcaat 361 ggcacgatat aaatagctcc attttccttt tattttgatg tacgtctcat caatacgcca 421 tttgtaataa gcttttttat gctttttctt ccaaatttga tataaaattg gggcatattc 481 ttgaacccaa cggtagaccg ttgaatgatg aacgtttaca ccacgtcccc ttaatatttc 541 agatatatca cgataactca atgcatatct tagatagtag ccaacggcta cagtgataac 601 atccttgtta aattgtttat atctgaaata gttcatacag aagactcctt tttgttaaaa 661 ttatactata aattcaactt tgcaacagaa ccgtaaagat tatacacctt tttaggtgta 721 taatctcttt tttttgaaaa tttaacttca aaaactaatt aaatagagat aatggattgt 781 tttccatatt ttttcttctc ataatagttg agtgcatttc ttcaagttca gcaatgatac 841 ttctcattaa ataaccttcc atattagcta ctttttcttg ttttttaaca agataacctt 901 taaatcgttt taaaaccaat agtaattctt catctatatc ttctaacata tagaaagtat 961 cgtatttgtt attaaatgat tttttagctt ttaaaataac agatttaata cttttaactt 1021 cttcatagct gaaattatta atataacttt taattaattc ggggagttct tgaagttcta 1081 tatatttaag agattcttta tcatgaatat ttgaaaagtg atttgaatga tttgaatgtt 1141 gatttgtatc attcatatta ttcatatcat tactttcagt atcaataaaa tcagtatcaa 1201 taaaatcagt atcatttgtg tgtccttttg acatttctaa acgtgtcctt ttgacattac 1261 gagacgtgtc cttttgacat ttctggacgt gtccttttga cacttccttg tcttgtaagg 1321 cctcaacttc attttcagcc ttatctattt cataaatatc atttttagtt atggctggtt 1381 ttaataaata aagtagattt ggtttgttta aaccctgcct tttttggatt agtaaatcta 1441 cattttctaa ttctttttta attttagtga tttttttgtt cccacaattt aatatcactt 1501 ctaaatcagc aactgtataa atgaaatata tgttaccttc tgtatctatc cagttatttt 1561 taatagataa ttgtaaacga tctctcaata ttgcgtaagc aattttagcg tcattcgata 1621 aatctttata attaggatta gtaaaaaata ctttaggtaa ttggtaaaag cgttctttat 1681 aattttcttc tactgtaaaa aattgtttag acatgataaa aactccttta aatgtatatt 1741 taaggagttt aagtaatatc gttatttaca agtgatgaat aaggttatat aatgttatcc 1801 gttatatata acttaaagac ttgttgcttt tatcttgtaa tgtatcgagg ttgtatatat 1861 aataaagata caaacaaatt gtaaaagtaa ataacacata ttatttaaac tctttagaca 1921 tctaacaacc gccaagcgtt aggtgtcttt ttctatgttc aatagtaaca ctattttaaa 1981 aaaattaaaa attttttgtt gcctcacagg tcaattatat gcttgtaatg aaatgtattt 2041 gataacaaat ctctaatata attaatatat ctaaaattat gaggccgata aatcatgaat 2101 gaataccgta tacaaaaact atatctctag taaaacaagt taaggatgca gtttatgcat 2161 cccttaactt acttattaaa taatttatag ctattgaaaa gagataagaa ttgttcaaag 2221 ctaatattgt ttaaatcgtc aattcctgca tgttttaagg aattgttaaa ttgatttttt 2281 gtaaatattt tcttgtattc tttgttaacc catttcataa cgaaataatt atacttctgt 2341 ttatctttgt gtgatattct tgattttttt ctatttaatc tgataagtga gctattcact 2401 ttaggtttag gatgaaaata ttctcttgga accatactta atatagaaat atcaacttct 2461 gccattaaaa ataatgcgaa tgagcgtttt gtatttaata atcttttagc aaacccgtat 2521 tccacgatta aataaatctc atcagctata ctatcaaaaa caattttgcg tattatatcc 2581 gtacttatgt tataaggtat attaccaaat attttatagg attggttttt aggaaattta 2641 aactgcaata tatccttgtt taaaacttgg aaattatcgt gatcaacaag tttattttct 2701 gtagttttgc ataatttatg gtctatttca atggcagtta cgaaattaca cctctgtact 2761 aattcaaggg taaaatgccc ttttcctgag ccgatttcaa agatattatc atgttcattt 2821 aatcttatat ttgtcattat tttatctata ttatgttttg aagtaataaa gttttgactg 2881 tgttttatat ttttctcgtt cattataacc ctctttattt tttcctcctt ataaaattag 2941 tataattata gcacgagctc tgataaatat gaacatgaga tctgttatcc acagaatcag 3001 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 3061 aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc 3121 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 3181 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 3241 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 3301 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 3361 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 3421 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 3481 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 3541 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 3601 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 3661 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 3721 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 3781 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct 3841 cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat 3901 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata 3961 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg 4021 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc 4081 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata 4141 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac 4201 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct 4261 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa 4321 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc 4381 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga 4441 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc 4501 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca 4561 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc 4621 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt 4681 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc 4741 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 4801 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc 4861 gcggccgctt ctagagcaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 4921 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 4981 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 5041 ttgtgtggaa ttgtgagcgg ataacaattt cacacatact agagaaagag gagaaatact 5101 agatggcttc ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag 5161 gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag 5221 gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca 5281 tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc 5341 cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg 5401 aagacggtgg tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct 5461 acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa 5521 ccatgggttg ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg 5581 aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca 5641 cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg 5701 acatcacctc ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc 5761 gtcactccac cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag 5821 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 5881 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 5941 ttata //