LOCUS       BBa_K1326000            1839 bp    DNA     linear       07-May-2024
DEFINITION  Plac + CTH1 + YCF54
ACCESSION   BBa_K1326000
VERSION     BBa_K1326000.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             214..224
                     /label=RBS
     CDS             233..1354
                     /label=CTH1
     CDS             209..1360
                     /label=BBa_K1080005
     RBS             1374..1384
                     /label=RBS
     CDS             1393..1839
                     /label=25
     CDS             1369..1839
                     /label=BBa_K1080010
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagag ctttaagaag gagatataca taatggtggc
      241 tgctaccgct gctccccagg aggttgaggg cttcaaggtt atgcgtgacg gcatcaaggt
      301 tgcttccgat gagaccctgc tcactcctcg cttctacacc accgacttcg atgagatgga
      361 gcgtctgttc agcctggagc tgaacaagaa catggacatg gaggagttcg aggcgatgct
      421 gaacgagttc aagctggact acaaccagcg tcacttcgtc cgcaacgaga ccttcaagga
      481 ggcagcggag aagattcagg gtcccacccg caagatcttc attgagttcc tggagcgctc
      541 gtgcactgct gagttctcgg gtttcctgct ctacaaggag ctgggtcgtc gtctgaaggc
      601 cacgaaccct gtggtcgcgg agatcttcac cctgatgtcc cgtgacgagg cacgtcacgc
      661 tggcttcctg aacaaggcca tgtccgactt caacctggct ctggacctgg gtttcctgac
      721 caagaaccgc aagtacacct tcttcaagcc caagttcatc ttctacgcca cttacctgtc
      781 ggagaagatt ggctactggc gttacatttc tatctaccgt cacctgcaac gcaacccaga
      841 caaccagctg tatccgctgt tcgagtactt cgagaactgg tgccaggacg agaaccgtca
      901 cggtgacttc ttcaccgcag tgctgaaggc acgtccggag atggtcaacg actgggctgc
      961 caagctgtgg tcgcgcttct tctgcctgtc ggtgtacatc accatgtacc tgaacgacca
     1021 ccagcgtgac gcattctaca gctctcttgg actgaacacc acacagttca accagcacgt
     1081 gatcatcgag accaacaagt ctactgagcg tatcttcccc gctgtgccgg atgtggagaa
     1141 ccccgagttc ttccgtcgta tggacctgct ggtcaagtac aacgcacagc tggtgaacat
     1201 tggaagcatg aacctgccaa gtccaatcaa ggctatcatg aaggcaccaa ttctggagcg
     1261 catggtcgct gaggtgttcc aggtattcat catgacacct aaggagtctg gctcgtacga
     1321 cctggatgcc aacaagaccg ctctggtcta ctaataataa tactagagag ctttaagaag
     1381 gagatataca taatggctcc agctgctgca tcggcagaca aggctaccgc tgcggagtac
     1441 tatgctctgg tgtgcaacgc tgagtggttc ttcatggatc cacagaacga gtctgtggct
     1501 gagcagctgc gcgagaaggt tcgcttcttc aaggagcaga acaaggagcg cgacttcttc
     1561 atcgtgccta accctaagtg gcttgacgct aagttccctg agcaggctaa gcaggtgaag
     1621 cgtccatgcg tggcactggt ctctaccgac aagatgtgga tcacgttcat gaagctgcgt
     1681 ctggatcgtg tgctgaagat cgaccttaag tctatgcctg catcggaggt gctggcagct
     1741 ggtgaagctc tgcctgactt caagcctgat ggtaagtgga ctgctcctta cgcccggtac
     1801 actcctggtt ggtggaacgt gttcctgcct aaccattaa
//