BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961224 1 -35 range1961224 1 137 142 annotation1961227 1 start range1961227 1 173 173 annotation1961225 1 -10 range1961225 1 161 166 BBa_K1080005 1 "CTH1" CTH1 2013-09-21T11:00:00Z 2016-10-20T07:03:00Z Chlamydomonas reinhardtii DSTGHGGTRE false false _1389_ 7708 18612 9 In stock false RETHYDYRHTE false Macquarie University annotation2525678 1 CTH1 range2525678 1 25 1146 annotation2525677 1 RBS range2525677 1 6 16 BBa_K1080010 1 "YCF" YCF54 2013-09-21T11:00:00Z 2016-10-20T07:15:17Z Chlamydomonas reinhardtii Long Description here false false _1389_ 7708 18463 9 In stock false Design Considerations here false Macquarie University annotation2525683 1 RBS range2525683 1 6 16 annotation2525684 1 25 range2525684 1 25 471 BBa_K1326000 1 BBa_K1326000 Plac + CTH1 + YCF54 2014-09-28T11:00:00Z 2015-05-08T01:09:54Z Chlamydomonas reinhardtii The genes CTH1 (copper targeting protein) and YCF54 make up the oxidative cyclase pathway. Their proteins create the fifth ring in the chlorophyll molecule. false false _1701_ 0 23617 9 It's complicated true Codon optimisation false Leah Simmons component2427706 1 BBa_R0010 component2427714 1 BBa_K1080010 component2427713 1 BBa_K1080005 annotation2427706 1 BBa_R0010 range2427706 1 1 200 annotation2427713 1 BBa_K1080005 range2427713 1 209 1360 annotation2427714 1 BBa_K1080010 range2427714 1 1369 1839 BBa_K1326000_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagagctttaagaaggagatatacataatggtggctgctaccgctgctccccaggaggttgagggcttcaaggttatgcgtgacggcatcaaggttgcttccgatgagaccctgctcactcctcgcttctacaccaccgacttcgatgagatggagcgtctgttcagcctggagctgaacaagaacatggacatggaggagttcgaggcgatgctgaacgagttcaagctggactacaaccagcgtcacttcgtccgcaacgagaccttcaaggaggcagcggagaagattcagggtcccacccgcaagatcttcattgagttcctggagcgctcgtgcactgctgagttctcgggtttcctgctctacaaggagctgggtcgtcgtctgaaggccacgaaccctgtggtcgcggagatcttcaccctgatgtcccgtgacgaggcacgtcacgctggcttcctgaacaaggccatgtccgacttcaacctggctctggacctgggtttcctgaccaagaaccgcaagtacaccttcttcaagcccaagttcatcttctacgccacttacctgtcggagaagattggctactggcgttacatttctatctaccgtcacctgcaacgcaacccagacaaccagctgtatccgctgttcgagtacttcgagaactggtgccaggacgagaaccgtcacggtgacttcttcaccgcagtgctgaaggcacgtccggagatggtcaacgactgggctgccaagctgtggtcgcgcttcttctgcctgtcggtgtacatcaccatgtacctgaacgaccaccagcgtgacgcattctacagctctcttggactgaacaccacacagttcaaccagcacgtgatcatcgagaccaacaagtctactgagcgtatcttccccgctgtgccggatgtggagaaccccgagttcttccgtcgtatggacctgctggtcaagtacaacgcacagctggtgaacattggaagcatgaacctgccaagtccaatcaaggctatcatgaaggcaccaattctggagcgcatggtcgctgaggtgttccaggtattcatcatgacacctaaggagtctggctcgtacgacctggatgccaacaagaccgctctggtctactaataataatactagagagctttaagaaggagatatacataatggctccagctgctgcatcggcagacaaggctaccgctgcggagtactatgctctggtgtgcaacgctgagtggttcttcatggatccacagaacgagtctgtggctgagcagctgcgcgagaaggttcgcttcttcaaggagcagaacaaggagcgcgacttcttcatcgtgcctaaccctaagtggcttgacgctaagttccctgagcaggctaagcaggtgaagcgtccatgcgtggcactggtctctaccgacaagatgtggatcacgttcatgaagctgcgtctggatcgtgtgctgaagatcgaccttaagtctatgcctgcatcggaggtgctggcagctggtgaagctctgcctgacttcaagcctgatggtaagtggactgctccttacgcccggtacactcctggttggtggaacgtgttcctgcctaaccattaa BBa_K1080010_sequence 1 agctttaagaaggagatatacataatggctccagctgctgcatcggcagacaaggctaccgctgcggagtactatgctctggtgtgcaacgctgagtggttcttcatggatccacagaacgagtctgtggctgagcagctgcgcgagaaggttcgcttcttcaaggagcagaacaaggagcgcgacttcttcatcgtgcctaaccctaagtggcttgacgctaagttccctgagcaggctaagcaggtgaagcgtccatgcgtggcactggtctctaccgacaagatgtggatcacgttcatgaagctgcgtctggatcgtgtgctgaagatcgaccttaagtctatgcctgcatcggaggtgctggcagctggtgaagctctgcctgacttcaagcctgatggtaagtggactgctccttacgcccggtacactcctggttggtggaacgtgttcctgcctaaccattaa BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K1080005_sequence 1 agctttaagaaggagatatacataatggtggctgctaccgctgctccccaggaggttgagggcttcaaggttatgcgtgacggcatcaaggttgcttccgatgagaccctgctcactcctcgcttctacaccaccgacttcgatgagatggagcgtctgttcagcctggagctgaacaagaacatggacatggaggagttcgaggcgatgctgaacgagttcaagctggactacaaccagcgtcacttcgtccgcaacgagaccttcaaggaggcagcggagaagattcagggtcccacccgcaagatcttcattgagttcctggagcgctcgtgcactgctgagttctcgggtttcctgctctacaaggagctgggtcgtcgtctgaaggccacgaaccctgtggtcgcggagatcttcaccctgatgtcccgtgacgaggcacgtcacgctggcttcctgaacaaggccatgtccgacttcaacctggctctggacctgggtttcctgaccaagaaccgcaagtacaccttcttcaagcccaagttcatcttctacgccacttacctgtcggagaagattggctactggcgttacatttctatctaccgtcacctgcaacgcaacccagacaaccagctgtatccgctgttcgagtacttcgagaactggtgccaggacgagaaccgtcacggtgacttcttcaccgcagtgctgaaggcacgtccggagatggtcaacgactgggctgccaagctgtggtcgcgcttcttctgcctgtcggtgtacatcaccatgtacctgaacgaccaccagcgtgacgcattctacagctctcttggactgaacaccacacagttcaaccagcacgtgatcatcgagaccaacaagtctactgagcgtatcttccccgctgtgccggatgtggagaaccccgagttcttccgtcgtatggacctgctggtcaagtacaacgcacagctggtgaacattggaagcatgaacctgccaagtccaatcaaggctatcatgaaggcaccaattctggagcgcatggtcgctgaggtgttccaggtattcatcatgacacctaaggagtctggctcgtacgacctggatgccaacaagaccgctctggtctactaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z