BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961224
1
-35
range1961224
1
137
142
annotation1961227
1
start
range1961227
1
173
173
annotation1961225
1
-10
range1961225
1
161
166
BBa_K1080005
1
"CTH1"
CTH1
2013-09-21T11:00:00Z
2016-10-20T07:03:00Z
Chlamydomonas reinhardtii
DSTGHGGTRE
false
false
_1389_
7708
18612
9
In stock
false
RETHYDYRHTE
false
Macquarie University
annotation2525678
1
CTH1
range2525678
1
25
1146
annotation2525677
1
RBS
range2525677
1
6
16
BBa_K1080010
1
"YCF"
YCF54
2013-09-21T11:00:00Z
2016-10-20T07:15:17Z
Chlamydomonas reinhardtii
Long Description here
false
false
_1389_
7708
18463
9
In stock
false
Design Considerations here
false
Macquarie University
annotation2525683
1
RBS
range2525683
1
6
16
annotation2525684
1
25
range2525684
1
25
471
BBa_K1326000
1
BBa_K1326000
Plac + CTH1 + YCF54
2014-09-28T11:00:00Z
2015-05-08T01:09:54Z
Chlamydomonas reinhardtii
The genes CTH1 (copper targeting protein) and YCF54 make up the oxidative cyclase pathway. Their proteins create the fifth ring in the chlorophyll molecule.
false
false
_1701_
0
23617
9
It's complicated
true
Codon optimisation
false
Leah Simmons
component2427706
1
BBa_R0010
component2427714
1
BBa_K1080010
component2427713
1
BBa_K1080005
annotation2427706
1
BBa_R0010
range2427706
1
1
200
annotation2427713
1
BBa_K1080005
range2427713
1
209
1360
annotation2427714
1
BBa_K1080010
range2427714
1
1369
1839
BBa_K1326000_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagagctttaagaaggagatatacataatggtggctgctaccgctgctccccaggaggttgagggcttcaaggttatgcgtgacggcatcaaggttgcttccgatgagaccctgctcactcctcgcttctacaccaccgacttcgatgagatggagcgtctgttcagcctggagctgaacaagaacatggacatggaggagttcgaggcgatgctgaacgagttcaagctggactacaaccagcgtcacttcgtccgcaacgagaccttcaaggaggcagcggagaagattcagggtcccacccgcaagatcttcattgagttcctggagcgctcgtgcactgctgagttctcgggtttcctgctctacaaggagctgggtcgtcgtctgaaggccacgaaccctgtggtcgcggagatcttcaccctgatgtcccgtgacgaggcacgtcacgctggcttcctgaacaaggccatgtccgacttcaacctggctctggacctgggtttcctgaccaagaaccgcaagtacaccttcttcaagcccaagttcatcttctacgccacttacctgtcggagaagattggctactggcgttacatttctatctaccgtcacctgcaacgcaacccagacaaccagctgtatccgctgttcgagtacttcgagaactggtgccaggacgagaaccgtcacggtgacttcttcaccgcagtgctgaaggcacgtccggagatggtcaacgactgggctgccaagctgtggtcgcgcttcttctgcctgtcggtgtacatcaccatgtacctgaacgaccaccagcgtgacgcattctacagctctcttggactgaacaccacacagttcaaccagcacgtgatcatcgagaccaacaagtctactgagcgtatcttccccgctgtgccggatgtggagaaccccgagttcttccgtcgtatggacctgctggtcaagtacaacgcacagctggtgaacattggaagcatgaacctgccaagtccaatcaaggctatcatgaaggcaccaattctggagcgcatggtcgctgaggtgttccaggtattcatcatgacacctaaggagtctggctcgtacgacctggatgccaacaagaccgctctggtctactaataataatactagagagctttaagaaggagatatacataatggctccagctgctgcatcggcagacaaggctaccgctgcggagtactatgctctggtgtgcaacgctgagtggttcttcatggatccacagaacgagtctgtggctgagcagctgcgcgagaaggttcgcttcttcaaggagcagaacaaggagcgcgacttcttcatcgtgcctaaccctaagtggcttgacgctaagttccctgagcaggctaagcaggtgaagcgtccatgcgtggcactggtctctaccgacaagatgtggatcacgttcatgaagctgcgtctggatcgtgtgctgaagatcgaccttaagtctatgcctgcatcggaggtgctggcagctggtgaagctctgcctgacttcaagcctgatggtaagtggactgctccttacgcccggtacactcctggttggtggaacgtgttcctgcctaaccattaa
BBa_K1080010_sequence
1
agctttaagaaggagatatacataatggctccagctgctgcatcggcagacaaggctaccgctgcggagtactatgctctggtgtgcaacgctgagtggttcttcatggatccacagaacgagtctgtggctgagcagctgcgcgagaaggttcgcttcttcaaggagcagaacaaggagcgcgacttcttcatcgtgcctaaccctaagtggcttgacgctaagttccctgagcaggctaagcaggtgaagcgtccatgcgtggcactggtctctaccgacaagatgtggatcacgttcatgaagctgcgtctggatcgtgtgctgaagatcgaccttaagtctatgcctgcatcggaggtgctggcagctggtgaagctctgcctgacttcaagcctgatggtaagtggactgctccttacgcccggtacactcctggttggtggaacgtgttcctgcctaaccattaa
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_K1080005_sequence
1
agctttaagaaggagatatacataatggtggctgctaccgctgctccccaggaggttgagggcttcaaggttatgcgtgacggcatcaaggttgcttccgatgagaccctgctcactcctcgcttctacaccaccgacttcgatgagatggagcgtctgttcagcctggagctgaacaagaacatggacatggaggagttcgaggcgatgctgaacgagttcaagctggactacaaccagcgtcacttcgtccgcaacgagaccttcaaggaggcagcggagaagattcagggtcccacccgcaagatcttcattgagttcctggagcgctcgtgcactgctgagttctcgggtttcctgctctacaaggagctgggtcgtcgtctgaaggccacgaaccctgtggtcgcggagatcttcaccctgatgtcccgtgacgaggcacgtcacgctggcttcctgaacaaggccatgtccgacttcaacctggctctggacctgggtttcctgaccaagaaccgcaagtacaccttcttcaagcccaagttcatcttctacgccacttacctgtcggagaagattggctactggcgttacatttctatctaccgtcacctgcaacgcaacccagacaaccagctgtatccgctgttcgagtacttcgagaactggtgccaggacgagaaccgtcacggtgacttcttcaccgcagtgctgaaggcacgtccggagatggtcaacgactgggctgccaagctgtggtcgcgcttcttctgcctgtcggtgtacatcaccatgtacctgaacgaccaccagcgtgacgcattctacagctctcttggactgaacaccacacagttcaaccagcacgtgatcatcgagaccaacaagtctactgagcgtatcttccccgctgtgccggatgtggagaaccccgagttcttccgtcgtatggacctgctggtcaagtacaacgcacagctggtgaacattggaagcatgaacctgccaagtccaatcaaggctatcatgaaggcaccaattctggagcgcatggtcgctgaggtgttccaggtattcatcatgacacctaaggagtctggctcgtacgacctggatgccaacaagaccgctctggtctactaataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z