BBa_K1326001 1 ChlM ChlM 2014-09-30T11:00:00Z 2015-05-08T01:09:54Z Chlamydomonas reinhardtii Mg protoporphyrin IX S-adenosyl methionine O-methyl transferase - Magnesium-protoporphyrin O-methyltransferase (ChlM) [PMID: 12828371; 12489983; 4436384]; ChloroP 1.1 predicts cp location false false _1701_ 0 23617 9 Not in stock false Codon optimisation false Leah Simmons BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961227 1 start range1961227 1 173 173 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961225 1 -10 range1961225 1 161 166 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961224 1 -35 range1961224 1 137 142 BBa_K1080005 1 "CTH1" CTH1 2013-09-21T11:00:00Z 2016-10-20T07:03:00Z Chlamydomonas reinhardtii DSTGHGGTRE false false _1389_ 7708 18612 9 In stock false RETHYDYRHTE false Macquarie University annotation2525677 1 RBS range2525677 1 6 16 annotation2525678 1 CTH1 range2525678 1 25 1146 BBa_K1326002 1 BBa_K1326002 Plac + CTH1 + YCF54 + Plasto + ChlM 2014-10-07T11:00:00Z 2015-05-08T01:09:54Z Chlamydomonas reinhardtii Composite part incorporating the genes comprising the middle third of the chlorophyll biosynthetic pathway. CTH1 (copper targeting protein) and YCF54 make up the oxidative cyclase pathway. Their proteins create the fifth ring in the chlorophyll molecule. Plastocyanin is a copper-containing chloroplast precursor protein. Copper is involved in electron transfer on plastocyanin's functional site between cytochrome f (from cyctochrome b6f complex, from photosystem II) and P700+ (from photosystem I). ChlM is shorthand for Magnesium-protoporphyrin O-methyltransferase, which catalyses the methylation of magnesium protoporphyrin IX., frming magnesium protoporphyrin IX monomethylester. false false _1701_ 0 23617 9 It's complicated true Codon optimisation and attachment of BioBrick prefix and suffix. false Leah Simmons component2428880 1 BBa_K1326001 component2428870 1 BBa_R0010 component2428879 1 BBa_K1080006 component2428878 1 BBa_K1080010 component2428877 1 BBa_K1080005 annotation2428877 1 BBa_K1080005 range2428877 1 209 1360 annotation2428880 1 BBa_K1326001 range2428880 1 2180 3046 annotation2428870 1 BBa_R0010 range2428870 1 1 200 annotation2428878 1 BBa_K1080010 range2428878 1 1369 1839 annotation2428879 1 BBa_K1080006 range2428879 1 1848 2171 BBa_K1080006 1 "Plasto" Plastocyanin 2013-09-11T11:00:00Z 2016-10-20T07:04:30Z From genome of Chlamydomonas reinhardtii Copper binding protein false false _1389_ 7708 18463 9 In stock true Incorporated sequence overlap for Gibson assembly and no GC rich regions or restriction sites within sequence false Macquarie University annotation2525679 1 RBS range2525679 1 6 16 annotation2525680 1 Plasto range2525680 1 25 321 BBa_K1080010 1 "YCF" YCF54 2013-09-21T11:00:00Z 2016-10-20T07:15:17Z Chlamydomonas reinhardtii Long Description here false false _1389_ 7708 18463 9 In stock false Design Considerations here false Macquarie University annotation2525684 1 25 range2525684 1 25 471 annotation2525683 1 RBS range2525683 1 6 16 BBa_K1080010_sequence 1 agctttaagaaggagatatacataatggctccagctgctgcatcggcagacaaggctaccgctgcggagtactatgctctggtgtgcaacgctgagtggttcttcatggatccacagaacgagtctgtggctgagcagctgcgcgagaaggttcgcttcttcaaggagcagaacaaggagcgcgacttcttcatcgtgcctaaccctaagtggcttgacgctaagttccctgagcaggctaagcaggtgaagcgtccatgcgtggcactggtctctaccgacaagatgtggatcacgttcatgaagctgcgtctggatcgtgtgctgaagatcgaccttaagtctatgcctgcatcggaggtgctggcagctggtgaagctctgcctgacttcaagcctgatggtaagtggactgctccttacgcccggtacactcctggttggtggaacgtgttcctgcctaaccattaa BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K1326002_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagagctttaagaaggagatatacataatggtggctgctaccgctgctccccaggaggttgagggcttcaaggttatgcgtgacggcatcaaggttgcttccgatgagaccctgctcactcctcgcttctacaccaccgacttcgatgagatggagcgtctgttcagcctggagctgaacaagaacatggacatggaggagttcgaggcgatgctgaacgagttcaagctggactacaaccagcgtcacttcgtccgcaacgagaccttcaaggaggcagcggagaagattcagggtcccacccgcaagatcttcattgagttcctggagcgctcgtgcactgctgagttctcgggtttcctgctctacaaggagctgggtcgtcgtctgaaggccacgaaccctgtggtcgcggagatcttcaccctgatgtcccgtgacgaggcacgtcacgctggcttcctgaacaaggccatgtccgacttcaacctggctctggacctgggtttcctgaccaagaaccgcaagtacaccttcttcaagcccaagttcatcttctacgccacttacctgtcggagaagattggctactggcgttacatttctatctaccgtcacctgcaacgcaacccagacaaccagctgtatccgctgttcgagtacttcgagaactggtgccaggacgagaaccgtcacggtgacttcttcaccgcagtgctgaaggcacgtccggagatggtcaacgactgggctgccaagctgtggtcgcgcttcttctgcctgtcggtgtacatcaccatgtacctgaacgaccaccagcgtgacgcattctacagctctcttggactgaacaccacacagttcaaccagcacgtgatcatcgagaccaacaagtctactgagcgtatcttccccgctgtgccggatgtggagaaccccgagttcttccgtcgtatggacctgctggtcaagtacaacgcacagctggtgaacattggaagcatgaacctgccaagtccaatcaaggctatcatgaaggcaccaattctggagcgcatggtcgctgaggtgttccaggtattcatcatgacacctaaggagtctggctcgtacgacctggatgccaacaagaccgctctggtctactaataataatactagagagctttaagaaggagatatacataatggctccagctgctgcatcggcagacaaggctaccgctgcggagtactatgctctggtgtgcaacgctgagtggttcttcatggatccacagaacgagtctgtggctgagcagctgcgcgagaaggttcgcttcttcaaggagcagaacaaggagcgcgacttcttcatcgtgcctaaccctaagtggcttgacgctaagttccctgagcaggctaagcaggtgaagcgtccatgcgtggcactggtctctaccgacaagatgtggatcacgttcatgaagctgcgtctggatcgtgtgctgaagatcgaccttaagtctatgcctgcatcggaggtgctggcagctggtgaagctctgcctgacttcaagcctgatggtaagtggactgctccttacgcccggtacactcctggttggtggaacgtgttcctgcctaaccattaatactagagagctttaagaaggagatatacataatggctaccgtcaagctgggtgctgactctggtgctctggagttcgtccctaagaccctgaccatcaagtccggcgagaccgtgaacttcgtgaacaacgctggcttcccacacaacatcgtcttcgacgaggatgccatcccatccggtgtgaacgctgatgccatctcccgcgatgactacctgaacgcacctggtgagacctactcggtgaagctgaccgctgctggtgagtacggctactactgcgaacctcaccagggtgctggcatggtcggcaagatcattgtccagtaataatactagagagctttaagaaggagatatacataatggcttctgagattgctcaaactgccgatgttggctcgctgaccttcgcggttggtggagtgggtgctgttgtgggtctgggtgcactgcttgtcgctactgaccctcagaagcgtcgttcggagcagatgaagtcattcgacggtgatgagaaggaggcagtgaaggactacttcaacactgccggtttcgagcgctggcgtaagatctacggtgagacggacgaggtgaacaaggtgcagctggacatccgcactggccacgcacagacggtggacaaggtgctgcgctgggtggacgaggagggcagcgtgcagggcatcaccgtggcggactgcggctgcgggacaggcagcctggccatcccgctggcgctgcgcggcgccgccgtcagcgcctccgacatctccgccgccatggcgtctgaggcggagcagcgctaccagcaggcggtggcggccgggcagggcaaggcgcccaaggtggcgcccaagttcgaggcgctggacctggagtccgtcaagggcaagtacgacacggtcacttgcctggacgtgatgattcactacccccaggacaaggtggacgccatgatcacgcacctggcgggtctgtccgaccgccgcctcatcatctcgttcgcacccaagacgctgtcgtacagcatcctcaagcgcattggcgagctgttccccggccccagcaaggccacccgcgcctacctgcaccgtgaggaggacgtggaggctgccctcaagcgcgccggcttcaaggtcaccaagcgtgagatgaccgccaccagcttctacttctcgcgcctgctggaggccatccgcgagtaa BBa_K1080005_sequence 1 agctttaagaaggagatatacataatggtggctgctaccgctgctccccaggaggttgagggcttcaaggttatgcgtgacggcatcaaggttgcttccgatgagaccctgctcactcctcgcttctacaccaccgacttcgatgagatggagcgtctgttcagcctggagctgaacaagaacatggacatggaggagttcgaggcgatgctgaacgagttcaagctggactacaaccagcgtcacttcgtccgcaacgagaccttcaaggaggcagcggagaagattcagggtcccacccgcaagatcttcattgagttcctggagcgctcgtgcactgctgagttctcgggtttcctgctctacaaggagctgggtcgtcgtctgaaggccacgaaccctgtggtcgcggagatcttcaccctgatgtcccgtgacgaggcacgtcacgctggcttcctgaacaaggccatgtccgacttcaacctggctctggacctgggtttcctgaccaagaaccgcaagtacaccttcttcaagcccaagttcatcttctacgccacttacctgtcggagaagattggctactggcgttacatttctatctaccgtcacctgcaacgcaacccagacaaccagctgtatccgctgttcgagtacttcgagaactggtgccaggacgagaaccgtcacggtgacttcttcaccgcagtgctgaaggcacgtccggagatggtcaacgactgggctgccaagctgtggtcgcgcttcttctgcctgtcggtgtacatcaccatgtacctgaacgaccaccagcgtgacgcattctacagctctcttggactgaacaccacacagttcaaccagcacgtgatcatcgagaccaacaagtctactgagcgtatcttccccgctgtgccggatgtggagaaccccgagttcttccgtcgtatggacctgctggtcaagtacaacgcacagctggtgaacattggaagcatgaacctgccaagtccaatcaaggctatcatgaaggcaccaattctggagcgcatggtcgctgaggtgttccaggtattcatcatgacacctaaggagtctggctcgtacgacctggatgccaacaagaccgctctggtctactaataataa BBa_K1080006_sequence 1 agctttaagaaggagatatacataatggctaccgtcaagctgggtgctgactctggtgctctggagttcgtccctaagaccctgaccatcaagtccggcgagaccgtgaacttcgtgaacaacgctggcttcccacacaacatcgtcttcgacgaggatgccatcccatccggtgtgaacgctgatgccatctcccgcgatgactacctgaacgcacctggtgagacctactcggtgaagctgaccgctgctggtgagtacggctactactgcgaacctcaccagggtgctggcatggtcggcaagatcattgtccagtaataa BBa_K1326001_sequence 1 agctttaagaaggagatatacataatggcttctgagattgctcaaactgccgatgttggctcgctgaccttcgcggttggtggagtgggtgctgttgtgggtctgggtgcactgcttgtcgctactgaccctcagaagcgtcgttcggagcagatgaagtcattcgacggtgatgagaaggaggcagtgaaggactacttcaacactgccggtttcgagcgctggcgtaagatctacggtgagacggacgaggtgaacaaggtgcagctggacatccgcactggccacgcacagacggtggacaaggtgctgcgctgggtggacgaggagggcagcgtgcagggcatcaccgtggcggactgcggctgcgggacaggcagcctggccatcccgctggcgctgcgcggcgccgccgtcagcgcctccgacatctccgccgccatggcgtctgaggcggagcagcgctaccagcaggcggtggcggccgggcagggcaaggcgcccaaggtggcgcccaagttcgaggcgctggacctggagtccgtcaagggcaagtacgacacggtcacttgcctggacgtgatgattcactacccccaggacaaggtggacgccatgatcacgcacctggcgggtctgtccgaccgccgcctcatcatctcgttcgcacccaagacgctgtcgtacagcatcctcaagcgcattggcgagctgttccccggccccagcaaggccacccgcgcctacctgcaccgtgaggaggacgtggaggctgccctcaagcgcgccggcttcaaggtcaccaagcgtgagatgaccgccaccagcttctacttctcgcgcctgctggaggccatccgcgagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z