BBa_K1080008 1 "The Big P ChlP 2013-09-21T11:00:00Z 2016-10-20T07:22:47Z Chlamydomonas reinhardtii Long Description false false _1389_ 7708 18463 9 In stock false Design Consideration false Macquarie University annotation2525689 1 RBS range2525689 1 6 16 annotation2525690 1 ChlP range2525690 1 25 1293 BBa_K1080012 1 DVR1 DVR1 2013-09-21T11:00:00Z 2015-05-08T01:09:03Z Chlamydomonas reinhardtii THSHERRNBHYDF false false _1389_ 0 18612 9 In stock false STHSHTSHD false Macquarie University annotation2525688 1 DVR1 range2525688 1 25 1000 annotation2525687 1 RBS range2525687 1 6 16 BBa_K1080007 1 POR POR 2013-09-21T11:00:00Z 2016-10-20T07:16:42Z Chlamydomonas reinhardtii DFGBDTD false false _1389_ 7708 18612 9 It's complicated false DFGSGBRF false Macquarie University annotation2525685 1 RBS range2525685 1 6 16 annotation2525686 1 POR range2525686 1 25 1064 BBa_K1326003 1 BBa_K1326003 Plac + POR + DVR1 + ChlP + ChlG 2014-10-07T11:00:00Z 2015-05-08T01:09:54Z Chlamydomonas reinhardtii Composite part entailing genes at the end of the chlorophyll biosynthetic pathway, responsible for the construction of the chlorophyll molecule. Light-dependent protochlorophyllidereductase (POR), a chloroplast precursor, uses NADPH and light to convert protochlorophyllide to chlorophyllide. DVR1 is a chloroplast transit peptide. Plant geranylgeranylhydrogenase (ChlP) reduces free geranylgeranyldiphosphate to phytildiphosphate; this leads to the construction of the chlorophyll side-chain Chlorophyllsynthetase (ChlG) is a catalyst for the esterification of chlorophyllide with phytyl-pyrophosphate to make the chlorophyll molecule. false false _1701_ 0 23617 9 It's complicated true Codon optimisation and attachment of BioBrick prefix and suffix. false Leah Simmons component2427666 1 BBa_R0010 component2427673 1 BBa_K1080007 component2427676 1 BBa_K1080009 component2427674 1 BBa_K1080012 component2427675 1 BBa_K1080008 annotation2427666 1 BBa_R0010 range2427666 1 1 200 annotation2427674 1 BBa_K1080012 range2427674 1 1284 2389 annotation2427673 1 BBa_K1080007 range2427673 1 209 1275 annotation2427675 1 BBa_K1080008 range2427675 1 2398 3696 annotation2427676 1 BBa_K1080009 range2427676 1 3705 4754 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961224 1 -35 range1961224 1 137 142 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961227 1 start range1961227 1 173 173 annotation1961225 1 -10 range1961225 1 161 166 BBa_K1080009 1 "G" ChlG 2013-09-21T11:00:00Z 2016-10-20T07:21:10Z Chlamydomonas reinhardtii FSDBSDGBGDS false false _1389_ 7708 18612 9 In stock false DFSHGDSS false Macquarie University annotation2525692 1 ChlG range2525692 1 25 1044 annotation2525691 1 RBS range2525691 1 6 16 BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K1080008_sequence 1 agctttaagaaggagatatacataatggtcatcggtggtggtccatctggtgcatgcgcagcagagaccctggctaagggtggtgtggaaaccttcctgctggaacgcaagctggacaactgcaagccatgcggtggtgccatccctctgtgcatggttgaagagttcgacctgcctatggagattattgaccgtcgcgtcaccaagatgaagatgatctctccttccaaccgtgaagtcgatgtgggtaagacactcagcgaaacagagtggattggtatgtgccgtcgtgaagtgtttgatgactacctgcgtaaccgtgcacagaagctgggtgctaacatcgtgaacggtctgttcatgcgttctgagcagcagagcgctgaaggtcctttcaccatccactacaacagctacgaggatggtagcaagatgggtaagcctgctacactggaagtcgacatgatcatcggtgctgacggtgcaaacagccgtatcgccaaggagatcgacgctggcgagtacgactacgcaattgcattccaggaacgtatccgtatcccagatgacaagatgaagtactatgagaacctggctgagatgtacgttggtgacgacgtgtctccagacttctacggatgggtgttccctaagtacgaccacgttgcagttggtacaggtacagttgtcaacaagacagctatcaagcagtaccagcaggctacacgtgatcgctctaaggtcaagaccgaaggtggtaagatcatccgtgttgaggcacatccaatcccagagcatccacgtccacgtcgttgcaagggtcgtgttgcactggtcggtgacgctgctggttacgtgaccaagtgctctggtgaaggtatctacttcgctgctaagtctggtcgtatggcagctgaagctattgtggaaggttctgctaacggaactaagatgtgcggtgaagatgcaatccgtgtctacctggacaaatgggatcgtaagtactggacaacatacaaggttctggacatcctgcaaaaggttttctaccgatccaatcctgcacgtgaggcattcgtggagctgtgcgaagacagctacgtgcagaagatgacatttgactcgtacctgtacaagaccgtggtgccaggtaacccactggacgacgtcaagctgctggttcgtaccgtgtcttccatcctgcgttccaacgcactgcgtagcgttaactccaagtcggtgaacgtgtcgttcggctccaaggcgaacgaagaacgtgtcatggctgcgtaataataa BBa_K1080007_sequence 1 agctttaagaaggagatatacataatggttgtttgcgcagcaactgcaactgctccttctccatctctggctgataagttcaagcctaacgcaatcgcacgtgttccagcaacccagcagaagcagacagctatcatcaccggtgcaagctctggtctgggtctgaacgcagcaaaggctctggctgctaccggtgaatggcatgttgtaatggcatgccgtgacttcctcaaggctgagcaggctgctaagaaggtcggtatgccagcaggttcctactcaatcctgcacctggacctgtcctctctggaatctgttcgtcagttcgtgcagaacttcaaggcatctggtcgtcgtctggatgcactggtgtgcaacgctgctgtgtacctgccaacagcaaaggaacctcgcttcaccgcagacggcttcgaactgtctgtgggtaccaaccatcttggtcacttcctgctgaccaacctgctgctggatgacctgaagaacgcacctaacaagcaacctcgttgcattattgtcggttccatcacaggtaacaccaacactctggcaggtaacgtgccaccaaaggccaacctgggagatctgtcgggcctcgcagcaggtgtgccagcagctaatccaatgatggatggtcaagaatttaacggtgctaaggcctacaaggattccaaggttgcatgcatgatgaccgtgcgccagatgcatcagcgtttccatgatgcaaccggcatcaccttcgcatcgctgtacccaggctgcattgccgagactggtctgtttcgtgaacatgtgccactgttcaagactctgtttccaccattccagaagtacatcaccaagggttacgtgtcggaagaagaagctggtcgtcgtctggcagcagtcatctctgatcctaagctgaacaagtctggtgcatactggtcttggtcttccaccactgggtcgttcgacaacaagtgtcttaagaagtggccgaagaatccaaggcctcccacctgtgggaaatctctgccaagctggtgggcctgagcgcgtaataataa BBa_K1080009_sequence 1 agctttaagaaggagatatacataatgaaccagcaagctaccgaagaaaagagcgacaccaacagcgctgctcgccagatgctgggcatgaagggtgctgccttggagacggacatctggaaaatccgcgtgcagcttaccaagcctgtgacttggatccctctcatttggggtgttgcttgcggtgcagcagcttctggtcactaccagtggaacaaccctacacaaattgcgcagctgctgacctgcatgatgatgtctggtccattcctgactggctacactcagactattaacgactggtatgaccgtgagattgatgcaatcaacgaaccttaccgtccaattccatctggtcgtatctctgaacgtgacgtgatcgtgcagatctgggtgctgcttctaggtggtattggtctggcatacacacttgaccagtgggctggtcacacaacgcctgtgatgcttcagctgaccatcttcggctccttcatctcttacatctactctgcaccacctctcaagctcaagcagagcggttgggcaggtaactacgcacttggttcgtcatacatcgcactgccatggtgggcaggtcaggctctgtttggcactctgaccctggacgtgatggctctgaccatcgcatactcgctggcaggtctgggcattgccattgtgaacgacttcaagtccattgaaggtgacaggcagatgggtcttcagtcgctgcctgtggcgttcggtgtggacacggctaagtggatttgcgtgtccaccatcgacgtgacgcagcttggtgtagctgcctaccttgcctggggtctgcacgaggagctgtatggcgcggtgctgctggcgctcatcctgccgcagatctacttccagtacaagtacttcctgccagatccaatcgccaacgacgtcaagtaccaggcatccgcacagccattccttgtgttcggcctgctcacagcaggtctggcatgcggtcaccacgtcaacgcagtggcagcagcagcttcagcagctggtgcactgtaataataa BBa_K1326003_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagagctttaagaaggagatatacataatggttgtttgcgcagcaactgcaactgctccttctccatctctggctgataagttcaagcctaacgcaatcgcacgtgttccagcaacccagcagaagcagacagctatcatcaccggtgcaagctctggtctgggtctgaacgcagcaaaggctctggctgctaccggtgaatggcatgttgtaatggcatgccgtgacttcctcaaggctgagcaggctgctaagaaggtcggtatgccagcaggttcctactcaatcctgcacctggacctgtcctctctggaatctgttcgtcagttcgtgcagaacttcaaggcatctggtcgtcgtctggatgcactggtgtgcaacgctgctgtgtacctgccaacagcaaaggaacctcgcttcaccgcagacggcttcgaactgtctgtgggtaccaaccatcttggtcacttcctgctgaccaacctgctgctggatgacctgaagaacgcacctaacaagcaacctcgttgcattattgtcggttccatcacaggtaacaccaacactctggcaggtaacgtgccaccaaaggccaacctgggagatctgtcgggcctcgcagcaggtgtgccagcagctaatccaatgatggatggtcaagaatttaacggtgctaaggcctacaaggattccaaggttgcatgcatgatgaccgtgcgccagatgcatcagcgtttccatgatgcaaccggcatcaccttcgcatcgctgtacccaggctgcattgccgagactggtctgtttcgtgaacatgtgccactgttcaagactctgtttccaccattccagaagtacatcaccaagggttacgtgtcggaagaagaagctggtcgtcgtctggcagcagtcatctctgatcctaagctgaacaagtctggtgcatactggtcttggtcttccaccactgggtcgttcgacaacaagtgtcttaagaagtggccgaagaatccaaggcctcccacctgtgggaaatctctgccaagctggtgggcctgagcgcgtaataataatactagagagctttaagaaggagatatacataatggcaatggcagcatctcgtcaggctgttcgcgttgcagcagctgtggatgctgattaccgtaagcgcgaaccaaaggacgtgcgcgtgcttgttgtaggtccaacaggttacatcggtaagttcgtggtgaaggaactcgtgagccgtggttacaacgtggttgcattcgcacgtgagaatgcaggtatcaagggtaagatgggtcgtgaagatatcgtgaaggagttccatggtgcggaagtgcgtttcggttctgtgctggatccagcttcgctgcgtgacgttgcattcaaggacccagttgacgttgttgttagctgcctggcaagccgcacaggtggtaagaaggactcgtggctgatcgactacacagctactaagaacagcctggacgttgcacgcgcatctggtgcaaagcactttgtgctgctgtctgcaatctgcgtgcagaagccactgctggagttccagaaagccaagctccagttcgagtctgaccttcaggctgccggtgacatcacctattccatcgtgcgtcctaccgcattcttcaagtccattgctggtcagatcgacatcgtgaagaaaggtaacccatacgtcatgtttggtgacggtaacctggcagcatgcaaacctatcagcgaagctgacctggcttcattcattgcagattgcgtcaccgaacagaacaaggtcaacaaggtgctgcctatcggtggtccaagcaaggccttcacggctaagcagcaggctgatctgctgttcaacatcacaggtctgccacctaagtacttccctgtgcctgtggcactcatggatggtatgattggtctgttcgactctctggctaagctgttcccacagctggaagatagcgctgaatttgcacgtattggtaagtactatgccaccgaatccatgctggtgtacgacgaggcacgtggtgtgtaccggaagacgaaacgcctggttacggcaaggacacgctggaagacttcttctctcgtgcagtgaaggaaggtctgcaaggacaggaactgggtgaccaggcagtgtttggacaacaataataataatactagagagctttaagaaggagatatacataatggtcatcggtggtggtccatctggtgcatgcgcagcagagaccctggctaagggtggtgtggaaaccttcctgctggaacgcaagctggacaactgcaagccatgcggtggtgccatccctctgtgcatggttgaagagttcgacctgcctatggagattattgaccgtcgcgtcaccaagatgaagatgatctctccttccaaccgtgaagtcgatgtgggtaagacactcagcgaaacagagtggattggtatgtgccgtcgtgaagtgtttgatgactacctgcgtaaccgtgcacagaagctgggtgctaacatcgtgaacggtctgttcatgcgttctgagcagcagagcgctgaaggtcctttcaccatccactacaacagctacgaggatggtagcaagatgggtaagcctgctacactggaagtcgacatgatcatcggtgctgacggtgcaaacagccgtatcgccaaggagatcgacgctggcgagtacgactacgcaattgcattccaggaacgtatccgtatcccagatgacaagatgaagtactatgagaacctggctgagatgtacgttggtgacgacgtgtctccagacttctacggatgggtgttccctaagtacgaccacgttgcagttggtacaggtacagttgtcaacaagacagctatcaagcagtaccagcaggctacacgtgatcgctctaaggtcaagaccgaaggtggtaagatcatccgtgttgaggcacatccaatcccagagcatccacgtccacgtcgttgcaagggtcgtgttgcactggtcggtgacgctgctggttacgtgaccaagtgctctggtgaaggtatctacttcgctgctaagtctggtcgtatggcagctgaagctattgtggaaggttctgctaacggaactaagatgtgcggtgaagatgcaatccgtgtctacctggacaaatgggatcgtaagtactggacaacatacaaggttctggacatcctgcaaaaggttttctaccgatccaatcctgcacgtgaggcattcgtggagctgtgcgaagacagctacgtgcagaagatgacatttgactcgtacctgtacaagaccgtggtgccaggtaacccactggacgacgtcaagctgctggttcgtaccgtgtcttccatcctgcgttccaacgcactgcgtagcgttaactccaagtcggtgaacgtgtcgttcggctccaaggcgaacgaagaacgtgtcatggctgcgtaataataatactagagagctttaagaaggagatatacataatgaaccagcaagctaccgaagaaaagagcgacaccaacagcgctgctcgccagatgctgggcatgaagggtgctgccttggagacggacatctggaaaatccgcgtgcagcttaccaagcctgtgacttggatccctctcatttggggtgttgcttgcggtgcagcagcttctggtcactaccagtggaacaaccctacacaaattgcgcagctgctgacctgcatgatgatgtctggtccattcctgactggctacactcagactattaacgactggtatgaccgtgagattgatgcaatcaacgaaccttaccgtccaattccatctggtcgtatctctgaacgtgacgtgatcgtgcagatctgggtgctgcttctaggtggtattggtctggcatacacacttgaccagtgggctggtcacacaacgcctgtgatgcttcagctgaccatcttcggctccttcatctcttacatctactctgcaccacctctcaagctcaagcagagcggttgggcaggtaactacgcacttggttcgtcatacatcgcactgccatggtgggcaggtcaggctctgtttggcactctgaccctggacgtgatggctctgaccatcgcatactcgctggcaggtctgggcattgccattgtgaacgacttcaagtccattgaaggtgacaggcagatgggtcttcagtcgctgcctgtggcgttcggtgtggacacggctaagtggatttgcgtgtccaccatcgacgtgacgcagcttggtgtagctgcctaccttgcctggggtctgcacgaggagctgtatggcgcggtgctgctggcgctcatcctgccgcagatctacttccagtacaagtacttcctgccagatccaatcgccaacgacgtcaagtaccaggcatccgcacagccattccttgtgttcggcctgctcacagcaggtctggcatgcggtcaccacgtcaacgcagtggcagcagcagcttcagcagctggtgcactgtaataataa BBa_K1080012_sequence 1 agctttaagaaggagatatacataatggcaatggcagcatctcgtcaggctgttcgcgttgcagcagctgtggatgctgattaccgtaagcgcgaaccaaaggacgtgcgcgtgcttgttgtaggtccaacaggttacatcggtaagttcgtggtgaaggaactcgtgagccgtggttacaacgtggttgcattcgcacgtgagaatgcaggtatcaagggtaagatgggtcgtgaagatatcgtgaaggagttccatggtgcggaagtgcgtttcggttctgtgctggatccagcttcgctgcgtgacgttgcattcaaggacccagttgacgttgttgttagctgcctggcaagccgcacaggtggtaagaaggactcgtggctgatcgactacacagctactaagaacagcctggacgttgcacgcgcatctggtgcaaagcactttgtgctgctgtctgcaatctgcgtgcagaagccactgctggagttccagaaagccaagctccagttcgagtctgaccttcaggctgccggtgacatcacctattccatcgtgcgtcctaccgcattcttcaagtccattgctggtcagatcgacatcgtgaagaaaggtaacccatacgtcatgtttggtgacggtaacctggcagcatgcaaacctatcagcgaagctgacctggcttcattcattgcagattgcgtcaccgaacagaacaaggtcaacaaggtgctgcctatcggtggtccaagcaaggccttcacggctaagcagcaggctgatctgctgttcaacatcacaggtctgccacctaagtacttccctgtgcctgtggcactcatggatggtatgattggtctgttcgactctctggctaagctgttcccacagctggaagatagcgctgaatttgcacgtattggtaagtactatgccaccgaatccatgctggtgtacgacgaggcacgtggtgtgtaccggaagacgaaacgcctggttacggcaaggacacgctggaagacttcttctctcgtgcagtgaaggaaggtctgcaaggacaggaactgggtgaccaggcagtgtttggacaacaataataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z