LOCUS       BBa_K1326004            3486 bp    DNA     linear       22-Nov-2024
DEFINITION  Plac + ChlI1 + ChlD
ACCESSION   BBa_K1326004
VERSION     BBa_K1326004.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             214..224
                     /label=RBS
     CDS             233..1321
                     /label=ChlI1
     CDS             209..1324
                     /label=BBa_K1080000
     RBS             1338..1348
                     /label=RBS
     CDS             1357..3486
                     /label=ChlD
     CDS             1333..3486
                     /label=BBa_K1080002
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagag ctttaagaag gagatataca taatggcagc
      241 tactgaggtc aaggctgctg aaggccgtac tgagaaggag ctgggtcagg cacgtccaat
      301 ctttccattc accgccatcg ttggtcagga tgagatgaag ctggcgctga ttctgaacgt
      361 gatcgatcct aagatcggtg gtgtcatgat catgggtgac cgtggtactg gtaagtccac
      421 tactattcgt gcactggcag atctgctgcc tgaaatgcag gtggttgcta acgatccatt
      481 taactcggat ccaactgatc cagagctgat gagtgaagaa gttcgtaacc gtgtcaaggc
      541 aggtgaacag ctgccagtgt cttccaagaa gattcctatg gttgacctgc cactgggtgc
      601 tactgaagat cgtgtgtgcg gtaccatcga tatcgagaag gcactgactg aaggtgttaa
      661 ggcattcgag cctggtctgc tggctaaggc taaccgtggt atcctgtacg tggatgaagt
      721 caacctgctg gatgatcatc tggttgatgt tctgctggac tcggcagcat ctggttggaa
      781 caccgtggag cgtgaaggta tctccatcag ccacccagcc cgcttcatcc tggttggttc
      841 gggtaaccca gaggaaggtg aactgcgtcc acagctgctg gatcgcttcg gcatgcacgc
      901 tcagatcggt accgtcaaag atcctcgtct gcgtgtgcaa atcgtgtcgc agcgctcgac
      961 cttcgacgag aatccagccg cctttcgcaa ggactacgaa gctggtcaga tggcactgac
     1021 acagcgtatc gttgacgctc gtaagctgct gaagcaaggt gaagtcaact acgacttccg
     1081 cgtcaagatc agccagatct gctcggacct gaacgtggac ggcatccgtg gtgatatcgt
     1141 gactaaccgt gcagctaagg ctctggctgc attcgaaggt cgtaccgaag tgactccaga
     1201 agacatctac cgtgtcattc cactgtgcct gcgtcaccgt ctccgtaaag acccactggc
     1261 tgagatcgac gacggtgacc gtgtgcgtga gatcttcaag caggtgttcg gcatggagta
     1321 ataatactag agagctttaa gaaggagata tacataatgc gcgccatgaa ggtgtctgag
     1381 gaggactcca agggcttcga tgcggatgtg tcgacccgcc tggcccgctc gtaccctctg
     1441 gcggccgtgg tgggccagga caacatcaag caggcgctgc tgctgggcgc cgtggacacc
     1501 gggctgggcg gcatcgccat cgccggtcgc cgcggtaccg ccaagtccat catggctcgc
     1561 ggcctgcacg ctctgctgcc gcccattgag gtggtggagg gcagcatctg caacgccgac
     1621 cccgaggacc cccgctcctg ggaggctggc ctggctgaga agtatgcggg cggccctgtg
     1681 aagaccaaga tgcgctcggc gccgtttgtg cagatccctc tgggtgtgac tgaggaccgc
     1741 ttggtgggca ctgtggacat tgaggcgtcc atgaaggagg gcaagactgt gttccagccc
     1801 ggcctgctgg ctgaggcgca ccgcggcatc ctgtacgtgg acgagatcaa cctgctggat
     1861 gacggcattg ccaacctgct gctgtccatc ctgtcggacg gagtcaacgt ggtggagcgc
     1921 gagggcatct ccatcagcca cccctgccgg ccgctgctga ttgccaccta caaccccgag
     1981 gagggccctc tgcgtgagca cctgctggac cgcatcgcca ttggcctcag cgccgacgtc
     2041 cccagcacca gcgacgagcg cgtcaaggcc attgacgcag ccatccgctt ccaggacaag
     2101 ccgcaggaca ctattgacga caccgcggag ctcaccgacg ccctgcgcac ctcggtcatc
     2161 ctggctcgcg agtacctgaa ggacgtgacc atcgcgccgg agcaggtgac ctacattgtg
     2221 gaggaggcgc gccgcggcgg agtccagggg caccgcgcgg agctgtacgc ggtcaagtgt
     2281 gccaaggcgt gtgcggctct ggagggccgt gagcgtgtga acaaggatga cctgcgccag
     2341 gccgtgcagc tggtcatcct gccgcgcgcc accatcctgg accagccccc gcccgagcag
     2401 gagcagcccc cgccgccgcc cccgccccct cccccgccgc cgccgcagga ccaaatggag
     2461 gacgaggacc aggaggagaa ggaggacgag aaggaggagg aggagaagga gaacgaggac
     2521 caggacgagc ccgagatccc tcaggagttc atgtttgagt ccgagggcgt catcatggac
     2581 ccctccatcc tcatgttcgc gcagcagcag cagcgcgcgc agggccgctc cggccgcgcc
     2641 aagacgctca tcttcagcga cgaccgcggc cgctacatca agcccatgct gcccaagggt
     2701 gacaaggtca agcgcctggc agtggacgcc acgcttcgcg ccgccgcgcc ctaccagaag
     2761 attcgccggc agcaggccat cagcgagggc aaggtgcagc gcaaggtgta cgtggacaag
     2821 ccagacatgc gctccaagaa gctggcccgc aaggccggtg cgctggtgat ttttgttgtg
     2881 gacgcgtccg gctccatggc tctgaaccgc atgagcgccg ccaagggcgc ctgcatgcgc
     2941 ctgctggctg agtcgtacac cagccgcgac caggtgtgcc tcatcccctt ctacggcgac
     3001 aaggccgagg tgctgctgcc gccctccaag tccatcgcca tggcccgccg ccgcctggac
     3061 tcgctgccct gcggcggcgg ctcgcccctt gcgcacggcc tgtccacggc ggtacgtgtg
     3121 ggcatgcagg ccagccaggc gggcgaggtg ggccgcgtca tgatggtgct catcacggac
     3181 ggccgcgcca acgtcagcct ggccaagtcc aacgaggacc ccgaggcgct caagcccgac
     3241 gcgcccaagc ccaccgccga ctcgctgaag gacgaggtgc gcgacatggc caagaaggcc
     3301 gcgtccgccg gcatcaacgt gcttgtcatt gacacggaga acaagttcgt gagcaccggc
     3361 tttgcggagg agatctccaa ggcagcgcag ggcaagtact actacctgcc caacgccagc
     3421 gacgcagcaa tcgcagcagc agcatccggt gcaatggcag cagctaaggg aggatactag
     3481 caataa
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