BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961224 1 -35 range1961224 1 137 142 annotation1961227 1 start range1961227 1 173 173 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961222 1 BBa_R0010 range1961222 1 1 200 BBa_K1326004 1 BBa_K1326004 Plac + ChlI1 + ChlD 2014-10-07T11:00:00Z 2015-05-08T01:09:54Z Chlamydomonas reinhardtii Composite part incorporating the genes adding a Mg ion to protoporphyrin IX, as a part of the chlorophyll biosynthetic pathway. Magnesium chelatase subunit I (ChlI1) and Magnesium chelatase subunit D (ChlD) form an ATP-dependent complex (increased yield is seen if ChlI2 subunit is also present). This complex acts on protoporphyrin IX, bound to the ChlH protein, and inserts a magnesium ion which produces Mg-protoporphyrin IX. false false _1701_ 0 23617 9 It's complicated true Codon optimisation and attachment of BioBrick prefix and suffix. false Leah Simmons component2427723 1 BBa_K1080002 component2427715 1 BBa_R0010 component2427722 1 BBa_K1080000 annotation2427723 1 BBa_K1080002 range2427723 1 1333 3486 annotation2427715 1 BBa_R0010 range2427715 1 1 200 annotation2427722 1 BBa_K1080000 range2427722 1 209 1324 BBa_K1080000 1 "Eye 1" ChlI1 2013-09-21T11:00:00Z 2016-10-20T06:51:00Z Chlamydomonas reinhardtii hghghg false false _1389_ 7708 18612 9 In stock false jhjhjhj false Macquarie University annotation2525650 1 RBS range2525650 1 6 16 annotation2525651 1 ChlI1 range2525651 1 25 1113 BBa_K1080002 1 "D" ChlD 2013-09-21T11:00:00Z 2016-10-20T06:52:59Z Chlamydomonas reinhardtii fsdsd false false _1389_ 7708 18612 9 It's complicated true dfsgdggfd false Macquarie University annotation2525653 1 ChlD range2525653 1 25 2154 annotation2525652 1 RBS range2525652 1 6 16 BBa_K1080000_sequence 1 agctttaagaaggagatatacataatggcagctactgaggtcaaggctgctgaaggccgtactgagaaggagctgggtcaggcacgtccaatctttccattcaccgccatcgttggtcaggatgagatgaagctggcgctgattctgaacgtgatcgatcctaagatcggtggtgtcatgatcatgggtgaccgtggtactggtaagtccactactattcgtgcactggcagatctgctgcctgaaatgcaggtggttgctaacgatccatttaactcggatccaactgatccagagctgatgagtgaagaagttcgtaaccgtgtcaaggcaggtgaacagctgccagtgtcttccaagaagattcctatggttgacctgccactgggtgctactgaagatcgtgtgtgcggtaccatcgatatcgagaaggcactgactgaaggtgttaaggcattcgagcctggtctgctggctaaggctaaccgtggtatcctgtacgtggatgaagtcaacctgctggatgatcatctggttgatgttctgctggactcggcagcatctggttggaacaccgtggagcgtgaaggtatctccatcagccacccagcccgcttcatcctggttggttcgggtaacccagaggaaggtgaactgcgtccacagctgctggatcgcttcggcatgcacgctcagatcggtaccgtcaaagatcctcgtctgcgtgtgcaaatcgtgtcgcagcgctcgaccttcgacgagaatccagccgcctttcgcaaggactacgaagctggtcagatggcactgacacagcgtatcgttgacgctcgtaagctgctgaagcaaggtgaagtcaactacgacttccgcgtcaagatcagccagatctgctcggacctgaacgtggacggcatccgtggtgatatcgtgactaaccgtgcagctaaggctctggctgcattcgaaggtcgtaccgaagtgactccagaagacatctaccgtgtcattccactgtgcctgcgtcaccgtctccgtaaagacccactggctgagatcgacgacggtgaccgtgtgcgtgagatcttcaagcaggtgttcggcatggagtaataa BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K1326004_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagagctttaagaaggagatatacataatggcagctactgaggtcaaggctgctgaaggccgtactgagaaggagctgggtcaggcacgtccaatctttccattcaccgccatcgttggtcaggatgagatgaagctggcgctgattctgaacgtgatcgatcctaagatcggtggtgtcatgatcatgggtgaccgtggtactggtaagtccactactattcgtgcactggcagatctgctgcctgaaatgcaggtggttgctaacgatccatttaactcggatccaactgatccagagctgatgagtgaagaagttcgtaaccgtgtcaaggcaggtgaacagctgccagtgtcttccaagaagattcctatggttgacctgccactgggtgctactgaagatcgtgtgtgcggtaccatcgatatcgagaaggcactgactgaaggtgttaaggcattcgagcctggtctgctggctaaggctaaccgtggtatcctgtacgtggatgaagtcaacctgctggatgatcatctggttgatgttctgctggactcggcagcatctggttggaacaccgtggagcgtgaaggtatctccatcagccacccagcccgcttcatcctggttggttcgggtaacccagaggaaggtgaactgcgtccacagctgctggatcgcttcggcatgcacgctcagatcggtaccgtcaaagatcctcgtctgcgtgtgcaaatcgtgtcgcagcgctcgaccttcgacgagaatccagccgcctttcgcaaggactacgaagctggtcagatggcactgacacagcgtatcgttgacgctcgtaagctgctgaagcaaggtgaagtcaactacgacttccgcgtcaagatcagccagatctgctcggacctgaacgtggacggcatccgtggtgatatcgtgactaaccgtgcagctaaggctctggctgcattcgaaggtcgtaccgaagtgactccagaagacatctaccgtgtcattccactgtgcctgcgtcaccgtctccgtaaagacccactggctgagatcgacgacggtgaccgtgtgcgtgagatcttcaagcaggtgttcggcatggagtaataatactagagagctttaagaaggagatatacataatgcgcgccatgaaggtgtctgaggaggactccaagggcttcgatgcggatgtgtcgacccgcctggcccgctcgtaccctctggcggccgtggtgggccaggacaacatcaagcaggcgctgctgctgggcgccgtggacaccgggctgggcggcatcgccatcgccggtcgccgcggtaccgccaagtccatcatggctcgcggcctgcacgctctgctgccgcccattgaggtggtggagggcagcatctgcaacgccgaccccgaggacccccgctcctgggaggctggcctggctgagaagtatgcgggcggccctgtgaagaccaagatgcgctcggcgccgtttgtgcagatccctctgggtgtgactgaggaccgcttggtgggcactgtggacattgaggcgtccatgaaggagggcaagactgtgttccagcccggcctgctggctgaggcgcaccgcggcatcctgtacgtggacgagatcaacctgctggatgacggcattgccaacctgctgctgtccatcctgtcggacggagtcaacgtggtggagcgcgagggcatctccatcagccacccctgccggccgctgctgattgccacctacaaccccgaggagggccctctgcgtgagcacctgctggaccgcatcgccattggcctcagcgccgacgtccccagcaccagcgacgagcgcgtcaaggccattgacgcagccatccgcttccaggacaagccgcaggacactattgacgacaccgcggagctcaccgacgccctgcgcacctcggtcatcctggctcgcgagtacctgaaggacgtgaccatcgcgccggagcaggtgacctacattgtggaggaggcgcgccgcggcggagtccaggggcaccgcgcggagctgtacgcggtcaagtgtgccaaggcgtgtgcggctctggagggccgtgagcgtgtgaacaaggatgacctgcgccaggccgtgcagctggtcatcctgccgcgcgccaccatcctggaccagcccccgcccgagcaggagcagcccccgccgccgcccccgccccctcccccgccgccgccgcaggaccaaatggaggacgaggaccaggaggagaaggaggacgagaaggaggaggaggagaaggagaacgaggaccaggacgagcccgagatccctcaggagttcatgtttgagtccgagggcgtcatcatggacccctccatcctcatgttcgcgcagcagcagcagcgcgcgcagggccgctccggccgcgccaagacgctcatcttcagcgacgaccgcggccgctacatcaagcccatgctgcccaagggtgacaaggtcaagcgcctggcagtggacgccacgcttcgcgccgccgcgccctaccagaagattcgccggcagcaggccatcagcgagggcaaggtgcagcgcaaggtgtacgtggacaagccagacatgcgctccaagaagctggcccgcaaggccggtgcgctggtgatttttgttgtggacgcgtccggctccatggctctgaaccgcatgagcgccgccaagggcgcctgcatgcgcctgctggctgagtcgtacaccagccgcgaccaggtgtgcctcatccccttctacggcgacaaggccgaggtgctgctgccgccctccaagtccatcgccatggcccgccgccgcctggactcgctgccctgcggcggcggctcgccccttgcgcacggcctgtccacggcggtacgtgtgggcatgcaggccagccaggcgggcgaggtgggccgcgtcatgatggtgctcatcacggacggccgcgccaacgtcagcctggccaagtccaacgaggaccccgaggcgctcaagcccgacgcgcccaagcccaccgccgactcgctgaaggacgaggtgcgcgacatggccaagaaggccgcgtccgccggcatcaacgtgcttgtcattgacacggagaacaagttcgtgagcaccggctttgcggaggagatctccaaggcagcgcagggcaagtactactacctgcccaacgccagcgacgcagcaatcgcagcagcagcatccggtgcaatggcagcagctaagggaggatactagcaataa BBa_K1080002_sequence 1 agctttaagaaggagatatacataatgcgcgccatgaaggtgtctgaggaggactccaagggcttcgatgcggatgtgtcgacccgcctggcccgctcgtaccctctggcggccgtggtgggccaggacaacatcaagcaggcgctgctgctgggcgccgtggacaccgggctgggcggcatcgccatcgccggtcgccgcggtaccgccaagtccatcatggctcgcggcctgcacgctctgctgccgcccattgaggtggtggagggcagcatctgcaacgccgaccccgaggacccccgctcctgggaggctggcctggctgagaagtatgcgggcggccctgtgaagaccaagatgcgctcggcgccgtttgtgcagatccctctgggtgtgactgaggaccgcttggtgggcactgtggacattgaggcgtccatgaaggagggcaagactgtgttccagcccggcctgctggctgaggcgcaccgcggcatcctgtacgtggacgagatcaacctgctggatgacggcattgccaacctgctgctgtccatcctgtcggacggagtcaacgtggtggagcgcgagggcatctccatcagccacccctgccggccgctgctgattgccacctacaaccccgaggagggccctctgcgtgagcacctgctggaccgcatcgccattggcctcagcgccgacgtccccagcaccagcgacgagcgcgtcaaggccattgacgcagccatccgcttccaggacaagccgcaggacactattgacgacaccgcggagctcaccgacgccctgcgcacctcggtcatcctggctcgcgagtacctgaaggacgtgaccatcgcgccggagcaggtgacctacattgtggaggaggcgcgccgcggcggagtccaggggcaccgcgcggagctgtacgcggtcaagtgtgccaaggcgtgtgcggctctggagggccgtgagcgtgtgaacaaggatgacctgcgccaggccgtgcagctggtcatcctgccgcgcgccaccatcctggaccagcccccgcccgagcaggagcagcccccgccgccgcccccgccccctcccccgccgccgccgcaggaccaaatggaggacgaggaccaggaggagaaggaggacgagaaggaggaggaggagaaggagaacgaggaccaggacgagcccgagatccctcaggagttcatgtttgagtccgagggcgtcatcatggacccctccatcctcatgttcgcgcagcagcagcagcgcgcgcagggccgctccggccgcgccaagacgctcatcttcagcgacgaccgcggccgctacatcaagcccatgctgcccaagggtgacaaggtcaagcgcctggcagtggacgccacgcttcgcgccgccgcgccctaccagaagattcgccggcagcaggccatcagcgagggcaaggtgcagcgcaaggtgtacgtggacaagccagacatgcgctccaagaagctggcccgcaaggccggtgcgctggtgatttttgttgtggacgcgtccggctccatggctctgaaccgcatgagcgccgccaagggcgcctgcatgcgcctgctggctgagtcgtacaccagccgcgaccaggtgtgcctcatccccttctacggcgacaaggccgaggtgctgctgccgccctccaagtccatcgccatggcccgccgccgcctggactcgctgccctgcggcggcggctcgccccttgcgcacggcctgtccacggcggtacgtgtgggcatgcaggccagccaggcgggcgaggtgggccgcgtcatgatggtgctcatcacggacggccgcgccaacgtcagcctggccaagtccaacgaggaccccgaggcgctcaagcccgacgcgcccaagcccaccgccgactcgctgaaggacgaggtgcgcgacatggccaagaaggccgcgtccgccggcatcaacgtgcttgtcattgacacggagaacaagttcgtgagcaccggctttgcggaggagatctccaaggcagcgcagggcaagtactactacctgcccaacgccagcgacgcagcaatcgcagcagcagcatccggtgcaatggcagcagctaagggaggatactagcaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z