BBa_K1080007 1 POR POR 2013-09-21T11:00:00Z 2016-10-20T07:16:42Z Chlamydomonas reinhardtii DFGBDTD false false _1389_ 7708 18612 9 It's complicated false DFGSGBRF false Macquarie University annotation2525686 1 POR range2525686 1 25 1064 annotation2525685 1 RBS range2525685 1 6 16 BBa_K1080012 1 DVR1 DVR1 2013-09-21T11:00:00Z 2015-05-08T01:09:03Z Chlamydomonas reinhardtii THSHERRNBHYDF false false _1389_ 0 18612 9 In stock false STHSHTSHD false Macquarie University annotation2525687 1 RBS range2525687 1 6 16 annotation2525688 1 DVR1 range2525688 1 25 1000 BBa_K1326006 1 BBa_K1326006 Plac + POR + DVR1 2014-10-15T11:00:00Z 2015-05-08T01:09:54Z Chlamydomonas reinhardtii Composite part made up of genes responsible for the terminal steps of the chlorophyll biosynthesis pathway false false _1701_ 0 23617 9 Not in stock false Codon optimisation false Leah Simmons component2427695 1 BBa_K1080012 component2427687 1 BBa_R0010 component2427694 1 BBa_K1080007 annotation2427695 1 BBa_K1080012 range2427695 1 1284 2389 annotation2427687 1 BBa_R0010 range2427687 1 1 200 annotation2427694 1 BBa_K1080007 range2427694 1 209 1275 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961227 1 start range1961227 1 173 173 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961224 1 -35 range1961224 1 137 142 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961225 1 -10 range1961225 1 161 166 BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K1326006_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagagctttaagaaggagatatacataatggttgtttgcgcagcaactgcaactgctccttctccatctctggctgataagttcaagcctaacgcaatcgcacgtgttccagcaacccagcagaagcagacagctatcatcaccggtgcaagctctggtctgggtctgaacgcagcaaaggctctggctgctaccggtgaatggcatgttgtaatggcatgccgtgacttcctcaaggctgagcaggctgctaagaaggtcggtatgccagcaggttcctactcaatcctgcacctggacctgtcctctctggaatctgttcgtcagttcgtgcagaacttcaaggcatctggtcgtcgtctggatgcactggtgtgcaacgctgctgtgtacctgccaacagcaaaggaacctcgcttcaccgcagacggcttcgaactgtctgtgggtaccaaccatcttggtcacttcctgctgaccaacctgctgctggatgacctgaagaacgcacctaacaagcaacctcgttgcattattgtcggttccatcacaggtaacaccaacactctggcaggtaacgtgccaccaaaggccaacctgggagatctgtcgggcctcgcagcaggtgtgccagcagctaatccaatgatggatggtcaagaatttaacggtgctaaggcctacaaggattccaaggttgcatgcatgatgaccgtgcgccagatgcatcagcgtttccatgatgcaaccggcatcaccttcgcatcgctgtacccaggctgcattgccgagactggtctgtttcgtgaacatgtgccactgttcaagactctgtttccaccattccagaagtacatcaccaagggttacgtgtcggaagaagaagctggtcgtcgtctggcagcagtcatctctgatcctaagctgaacaagtctggtgcatactggtcttggtcttccaccactgggtcgttcgacaacaagtgtcttaagaagtggccgaagaatccaaggcctcccacctgtgggaaatctctgccaagctggtgggcctgagcgcgtaataataatactagagagctttaagaaggagatatacataatggcaatggcagcatctcgtcaggctgttcgcgttgcagcagctgtggatgctgattaccgtaagcgcgaaccaaaggacgtgcgcgtgcttgttgtaggtccaacaggttacatcggtaagttcgtggtgaaggaactcgtgagccgtggttacaacgtggttgcattcgcacgtgagaatgcaggtatcaagggtaagatgggtcgtgaagatatcgtgaaggagttccatggtgcggaagtgcgtttcggttctgtgctggatccagcttcgctgcgtgacgttgcattcaaggacccagttgacgttgttgttagctgcctggcaagccgcacaggtggtaagaaggactcgtggctgatcgactacacagctactaagaacagcctggacgttgcacgcgcatctggtgcaaagcactttgtgctgctgtctgcaatctgcgtgcagaagccactgctggagttccagaaagccaagctccagttcgagtctgaccttcaggctgccggtgacatcacctattccatcgtgcgtcctaccgcattcttcaagtccattgctggtcagatcgacatcgtgaagaaaggtaacccatacgtcatgtttggtgacggtaacctggcagcatgcaaacctatcagcgaagctgacctggcttcattcattgcagattgcgtcaccgaacagaacaaggtcaacaaggtgctgcctatcggtggtccaagcaaggccttcacggctaagcagcaggctgatctgctgttcaacatcacaggtctgccacctaagtacttccctgtgcctgtggcactcatggatggtatgattggtctgttcgactctctggctaagctgttcccacagctggaagatagcgctgaatttgcacgtattggtaagtactatgccaccgaatccatgctggtgtacgacgaggcacgtggtgtgtaccggaagacgaaacgcctggttacggcaaggacacgctggaagacttcttctctcgtgcagtgaaggaaggtctgcaaggacaggaactgggtgaccaggcagtgtttggacaacaataataataa BBa_K1080007_sequence 1 agctttaagaaggagatatacataatggttgtttgcgcagcaactgcaactgctccttctccatctctggctgataagttcaagcctaacgcaatcgcacgtgttccagcaacccagcagaagcagacagctatcatcaccggtgcaagctctggtctgggtctgaacgcagcaaaggctctggctgctaccggtgaatggcatgttgtaatggcatgccgtgacttcctcaaggctgagcaggctgctaagaaggtcggtatgccagcaggttcctactcaatcctgcacctggacctgtcctctctggaatctgttcgtcagttcgtgcagaacttcaaggcatctggtcgtcgtctggatgcactggtgtgcaacgctgctgtgtacctgccaacagcaaaggaacctcgcttcaccgcagacggcttcgaactgtctgtgggtaccaaccatcttggtcacttcctgctgaccaacctgctgctggatgacctgaagaacgcacctaacaagcaacctcgttgcattattgtcggttccatcacaggtaacaccaacactctggcaggtaacgtgccaccaaaggccaacctgggagatctgtcgggcctcgcagcaggtgtgccagcagctaatccaatgatggatggtcaagaatttaacggtgctaaggcctacaaggattccaaggttgcatgcatgatgaccgtgcgccagatgcatcagcgtttccatgatgcaaccggcatcaccttcgcatcgctgtacccaggctgcattgccgagactggtctgtttcgtgaacatgtgccactgttcaagactctgtttccaccattccagaagtacatcaccaagggttacgtgtcggaagaagaagctggtcgtcgtctggcagcagtcatctctgatcctaagctgaacaagtctggtgcatactggtcttggtcttccaccactgggtcgttcgacaacaagtgtcttaagaagtggccgaagaatccaaggcctcccacctgtgggaaatctctgccaagctggtgggcctgagcgcgtaataataa BBa_K1080012_sequence 1 agctttaagaaggagatatacataatggcaatggcagcatctcgtcaggctgttcgcgttgcagcagctgtggatgctgattaccgtaagcgcgaaccaaaggacgtgcgcgtgcttgttgtaggtccaacaggttacatcggtaagttcgtggtgaaggaactcgtgagccgtggttacaacgtggttgcattcgcacgtgagaatgcaggtatcaagggtaagatgggtcgtgaagatatcgtgaaggagttccatggtgcggaagtgcgtttcggttctgtgctggatccagcttcgctgcgtgacgttgcattcaaggacccagttgacgttgttgttagctgcctggcaagccgcacaggtggtaagaaggactcgtggctgatcgactacacagctactaagaacagcctggacgttgcacgcgcatctggtgcaaagcactttgtgctgctgtctgcaatctgcgtgcagaagccactgctggagttccagaaagccaagctccagttcgagtctgaccttcaggctgccggtgacatcacctattccatcgtgcgtcctaccgcattcttcaagtccattgctggtcagatcgacatcgtgaagaaaggtaacccatacgtcatgtttggtgacggtaacctggcagcatgcaaacctatcagcgaagctgacctggcttcattcattgcagattgcgtcaccgaacagaacaaggtcaacaaggtgctgcctatcggtggtccaagcaaggccttcacggctaagcagcaggctgatctgctgttcaacatcacaggtctgccacctaagtacttccctgtgcctgtggcactcatggatggtatgattggtctgttcgactctctggctaagctgttcccacagctggaagatagcgctgaatttgcacgtattggtaagtactatgccaccgaatccatgctggtgtacgacgaggcacgtggtgtgtaccggaagacgaaacgcctggttacggcaaggacacgctggaagacttcttctctcgtgcagtgaaggaaggtctgcaaggacaggaactgggtgaccaggcagtgtttggacaacaataataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z