BBa_K1080012 1 DVR1 DVR1 2013-09-21T11:00:00Z 2015-05-08T01:09:03Z Chlamydomonas reinhardtii THSHERRNBHYDF false false _1389_ 0 18612 9 In stock false STHSHTSHD false Macquarie University annotation2525688 1 DVR1 range2525688 1 25 1000 annotation2525687 1 RBS range2525687 1 6 16 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961227 1 start range1961227 1 173 173 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961224 1 -35 range1961224 1 137 142 BBa_K1326007 1 BBa_K1326007 Plac + POR + DVR1 + ChlP 2014-10-15T11:00:00Z 2015-05-08T01:09:54Z Chlamydomonas reinhardtii Composite part made up of genes responsible for the terminal steps of the chlorophyll biosynthesis pathway false false _1701_ 0 23617 9 Not in stock false Codon optimisation false Leah Simmons component2427703 1 BBa_K1080007 component2427705 1 BBa_K1080008 component2427704 1 BBa_K1080012 component2427696 1 BBa_R0010 annotation2427696 1 BBa_R0010 range2427696 1 1 200 annotation2427705 1 BBa_K1080008 range2427705 1 2398 3696 annotation2427704 1 BBa_K1080012 range2427704 1 1284 2389 annotation2427703 1 BBa_K1080007 range2427703 1 209 1275 BBa_K1080008 1 "The Big P ChlP 2013-09-21T11:00:00Z 2016-10-20T07:22:47Z Chlamydomonas reinhardtii Long Description false false _1389_ 7708 18463 9 In stock false Design Consideration false Macquarie University annotation2525689 1 RBS range2525689 1 6 16 annotation2525690 1 ChlP range2525690 1 25 1293 BBa_K1080007 1 POR POR 2013-09-21T11:00:00Z 2016-10-20T07:16:42Z Chlamydomonas reinhardtii DFGBDTD false false _1389_ 7708 18612 9 It's complicated false DFGSGBRF false Macquarie University annotation2525686 1 POR range2525686 1 25 1064 annotation2525685 1 RBS range2525685 1 6 16 BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K1326007_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagagctttaagaaggagatatacataatggttgtttgcgcagcaactgcaactgctccttctccatctctggctgataagttcaagcctaacgcaatcgcacgtgttccagcaacccagcagaagcagacagctatcatcaccggtgcaagctctggtctgggtctgaacgcagcaaaggctctggctgctaccggtgaatggcatgttgtaatggcatgccgtgacttcctcaaggctgagcaggctgctaagaaggtcggtatgccagcaggttcctactcaatcctgcacctggacctgtcctctctggaatctgttcgtcagttcgtgcagaacttcaaggcatctggtcgtcgtctggatgcactggtgtgcaacgctgctgtgtacctgccaacagcaaaggaacctcgcttcaccgcagacggcttcgaactgtctgtgggtaccaaccatcttggtcacttcctgctgaccaacctgctgctggatgacctgaagaacgcacctaacaagcaacctcgttgcattattgtcggttccatcacaggtaacaccaacactctggcaggtaacgtgccaccaaaggccaacctgggagatctgtcgggcctcgcagcaggtgtgccagcagctaatccaatgatggatggtcaagaatttaacggtgctaaggcctacaaggattccaaggttgcatgcatgatgaccgtgcgccagatgcatcagcgtttccatgatgcaaccggcatcaccttcgcatcgctgtacccaggctgcattgccgagactggtctgtttcgtgaacatgtgccactgttcaagactctgtttccaccattccagaagtacatcaccaagggttacgtgtcggaagaagaagctggtcgtcgtctggcagcagtcatctctgatcctaagctgaacaagtctggtgcatactggtcttggtcttccaccactgggtcgttcgacaacaagtgtcttaagaagtggccgaagaatccaaggcctcccacctgtgggaaatctctgccaagctggtgggcctgagcgcgtaataataatactagagagctttaagaaggagatatacataatggcaatggcagcatctcgtcaggctgttcgcgttgcagcagctgtggatgctgattaccgtaagcgcgaaccaaaggacgtgcgcgtgcttgttgtaggtccaacaggttacatcggtaagttcgtggtgaaggaactcgtgagccgtggttacaacgtggttgcattcgcacgtgagaatgcaggtatcaagggtaagatgggtcgtgaagatatcgtgaaggagttccatggtgcggaagtgcgtttcggttctgtgctggatccagcttcgctgcgtgacgttgcattcaaggacccagttgacgttgttgttagctgcctggcaagccgcacaggtggtaagaaggactcgtggctgatcgactacacagctactaagaacagcctggacgttgcacgcgcatctggtgcaaagcactttgtgctgctgtctgcaatctgcgtgcagaagccactgctggagttccagaaagccaagctccagttcgagtctgaccttcaggctgccggtgacatcacctattccatcgtgcgtcctaccgcattcttcaagtccattgctggtcagatcgacatcgtgaagaaaggtaacccatacgtcatgtttggtgacggtaacctggcagcatgcaaacctatcagcgaagctgacctggcttcattcattgcagattgcgtcaccgaacagaacaaggtcaacaaggtgctgcctatcggtggtccaagcaaggccttcacggctaagcagcaggctgatctgctgttcaacatcacaggtctgccacctaagtacttccctgtgcctgtggcactcatggatggtatgattggtctgttcgactctctggctaagctgttcccacagctggaagatagcgctgaatttgcacgtattggtaagtactatgccaccgaatccatgctggtgtacgacgaggcacgtggtgtgtaccggaagacgaaacgcctggttacggcaaggacacgctggaagacttcttctctcgtgcagtgaaggaaggtctgcaaggacaggaactgggtgaccaggcagtgtttggacaacaataataataatactagagagctttaagaaggagatatacataatggtcatcggtggtggtccatctggtgcatgcgcagcagagaccctggctaagggtggtgtggaaaccttcctgctggaacgcaagctggacaactgcaagccatgcggtggtgccatccctctgtgcatggttgaagagttcgacctgcctatggagattattgaccgtcgcgtcaccaagatgaagatgatctctccttccaaccgtgaagtcgatgtgggtaagacactcagcgaaacagagtggattggtatgtgccgtcgtgaagtgtttgatgactacctgcgtaaccgtgcacagaagctgggtgctaacatcgtgaacggtctgttcatgcgttctgagcagcagagcgctgaaggtcctttcaccatccactacaacagctacgaggatggtagcaagatgggtaagcctgctacactggaagtcgacatgatcatcggtgctgacggtgcaaacagccgtatcgccaaggagatcgacgctggcgagtacgactacgcaattgcattccaggaacgtatccgtatcccagatgacaagatgaagtactatgagaacctggctgagatgtacgttggtgacgacgtgtctccagacttctacggatgggtgttccctaagtacgaccacgttgcagttggtacaggtacagttgtcaacaagacagctatcaagcagtaccagcaggctacacgtgatcgctctaaggtcaagaccgaaggtggtaagatcatccgtgttgaggcacatccaatcccagagcatccacgtccacgtcgttgcaagggtcgtgttgcactggtcggtgacgctgctggttacgtgaccaagtgctctggtgaaggtatctacttcgctgctaagtctggtcgtatggcagctgaagctattgtggaaggttctgctaacggaactaagatgtgcggtgaagatgcaatccgtgtctacctggacaaatgggatcgtaagtactggacaacatacaaggttctggacatcctgcaaaaggttttctaccgatccaatcctgcacgtgaggcattcgtggagctgtgcgaagacagctacgtgcagaagatgacatttgactcgtacctgtacaagaccgtggtgccaggtaacccactggacgacgtcaagctgctggttcgtaccgtgtcttccatcctgcgttccaacgcactgcgtagcgttaactccaagtcggtgaacgtgtcgttcggctccaaggcgaacgaagaacgtgtcatggctgcgtaataataa BBa_K1080008_sequence 1 agctttaagaaggagatatacataatggtcatcggtggtggtccatctggtgcatgcgcagcagagaccctggctaagggtggtgtggaaaccttcctgctggaacgcaagctggacaactgcaagccatgcggtggtgccatccctctgtgcatggttgaagagttcgacctgcctatggagattattgaccgtcgcgtcaccaagatgaagatgatctctccttccaaccgtgaagtcgatgtgggtaagacactcagcgaaacagagtggattggtatgtgccgtcgtgaagtgtttgatgactacctgcgtaaccgtgcacagaagctgggtgctaacatcgtgaacggtctgttcatgcgttctgagcagcagagcgctgaaggtcctttcaccatccactacaacagctacgaggatggtagcaagatgggtaagcctgctacactggaagtcgacatgatcatcggtgctgacggtgcaaacagccgtatcgccaaggagatcgacgctggcgagtacgactacgcaattgcattccaggaacgtatccgtatcccagatgacaagatgaagtactatgagaacctggctgagatgtacgttggtgacgacgtgtctccagacttctacggatgggtgttccctaagtacgaccacgttgcagttggtacaggtacagttgtcaacaagacagctatcaagcagtaccagcaggctacacgtgatcgctctaaggtcaagaccgaaggtggtaagatcatccgtgttgaggcacatccaatcccagagcatccacgtccacgtcgttgcaagggtcgtgttgcactggtcggtgacgctgctggttacgtgaccaagtgctctggtgaaggtatctacttcgctgctaagtctggtcgtatggcagctgaagctattgtggaaggttctgctaacggaactaagatgtgcggtgaagatgcaatccgtgtctacctggacaaatgggatcgtaagtactggacaacatacaaggttctggacatcctgcaaaaggttttctaccgatccaatcctgcacgtgaggcattcgtggagctgtgcgaagacagctacgtgcagaagatgacatttgactcgtacctgtacaagaccgtggtgccaggtaacccactggacgacgtcaagctgctggttcgtaccgtgtcttccatcctgcgttccaacgcactgcgtagcgttaactccaagtcggtgaacgtgtcgttcggctccaaggcgaacgaagaacgtgtcatggctgcgtaataataa BBa_K1080007_sequence 1 agctttaagaaggagatatacataatggttgtttgcgcagcaactgcaactgctccttctccatctctggctgataagttcaagcctaacgcaatcgcacgtgttccagcaacccagcagaagcagacagctatcatcaccggtgcaagctctggtctgggtctgaacgcagcaaaggctctggctgctaccggtgaatggcatgttgtaatggcatgccgtgacttcctcaaggctgagcaggctgctaagaaggtcggtatgccagcaggttcctactcaatcctgcacctggacctgtcctctctggaatctgttcgtcagttcgtgcagaacttcaaggcatctggtcgtcgtctggatgcactggtgtgcaacgctgctgtgtacctgccaacagcaaaggaacctcgcttcaccgcagacggcttcgaactgtctgtgggtaccaaccatcttggtcacttcctgctgaccaacctgctgctggatgacctgaagaacgcacctaacaagcaacctcgttgcattattgtcggttccatcacaggtaacaccaacactctggcaggtaacgtgccaccaaaggccaacctgggagatctgtcgggcctcgcagcaggtgtgccagcagctaatccaatgatggatggtcaagaatttaacggtgctaaggcctacaaggattccaaggttgcatgcatgatgaccgtgcgccagatgcatcagcgtttccatgatgcaaccggcatcaccttcgcatcgctgtacccaggctgcattgccgagactggtctgtttcgtgaacatgtgccactgttcaagactctgtttccaccattccagaagtacatcaccaagggttacgtgtcggaagaagaagctggtcgtcgtctggcagcagtcatctctgatcctaagctgaacaagtctggtgcatactggtcttggtcttccaccactgggtcgttcgacaacaagtgtcttaagaagtggccgaagaatccaaggcctcccacctgtgggaaatctctgccaagctggtgggcctgagcgcgtaataataa BBa_K1080012_sequence 1 agctttaagaaggagatatacataatggcaatggcagcatctcgtcaggctgttcgcgttgcagcagctgtggatgctgattaccgtaagcgcgaaccaaaggacgtgcgcgtgcttgttgtaggtccaacaggttacatcggtaagttcgtggtgaaggaactcgtgagccgtggttacaacgtggttgcattcgcacgtgagaatgcaggtatcaagggtaagatgggtcgtgaagatatcgtgaaggagttccatggtgcggaagtgcgtttcggttctgtgctggatccagcttcgctgcgtgacgttgcattcaaggacccagttgacgttgttgttagctgcctggcaagccgcacaggtggtaagaaggactcgtggctgatcgactacacagctactaagaacagcctggacgttgcacgcgcatctggtgcaaagcactttgtgctgctgtctgcaatctgcgtgcagaagccactgctggagttccagaaagccaagctccagttcgagtctgaccttcaggctgccggtgacatcacctattccatcgtgcgtcctaccgcattcttcaagtccattgctggtcagatcgacatcgtgaagaaaggtaacccatacgtcatgtttggtgacggtaacctggcagcatgcaaacctatcagcgaagctgacctggcttcattcattgcagattgcgtcaccgaacagaacaaggtcaacaaggtgctgcctatcggtggtccaagcaaggccttcacggctaagcagcaggctgatctgctgttcaacatcacaggtctgccacctaagtacttccctgtgcctgtggcactcatggatggtatgattggtctgttcgactctctggctaagctgttcccacagctggaagatagcgctgaatttgcacgtattggtaagtactatgccaccgaatccatgctggtgtacgacgaggcacgtggtgtgtaccggaagacgaaacgcctggttacggcaaggacacgctggaagacttcttctctcgtgcagtgaaggaaggtctgcaaggacaggaactgggtgaccaggcagtgtttggacaacaataataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z