BBa_K1328000 1 BBa_K1328000 HXT1-CMVmini 2014-10-08T11:00:00Z 2015-05-08T01:09:54Z HXT1 promoter sequence is cloned from S. cerevisiae genome via PCR. The CMV-mini promoter is cloned from a commercial plasmid. Glucose-regulated eukaryotic promoter sequence, activated under high glucose concentrations. Works in yeast as well as human fibroblast cells. HXT1 encodes a yeast hexose transporter, we fused its promoter sequence (-750 ~ -161 bp) to a CMV-mini promoter sequence (204 bp) to ensure glucose-sensitivity as well as high expression rates. Ferrer-Mart??nez A, Riera A, Jim??nez-Chillar??n JC, Herrero P, Moreno F, G??mez-Foix AM. A glucose response element from the S. cerevisiae hexose transporter HXT1 gene is sensitive to glucose in human fibroblasts. J Mol Biol. 2004 May 7;338(4):657-67. PubMed PMID: 15099735. false false _1703_ 0 15664 9 It's complicated false Region -750~-161 bp was selected from the HXT1 promoter for maximal glucose sensitivity. A CMV-mini promoter sequence was added to increase expression rate. false Tianyi Li annotation2406655 1 HXT-1 Promoter range2406655 1 1 586 annotation2406691 1 CMV-mini Promoter range2406691 1 587 790 BBa_K1328000_sequence 1 acgcaacttcgggtgttaagaaatattttgctacattagataatggtggagtttcctggcttgtcggataaaagccatcaaatgtcgcagcagctcatgtttacgtttgctgtcttctgcccacgtcatatgagtggtattcttctatcagcacttgatgaatattctttttctcatatatctgaaagacaaaagatcggcacggcaatgccctgctgcatttcttcctagtttttccgaatttccattacgtattggatcttgtgcgcatatttgtcagtccttcacggaaaaaaaaaaagagcactgggtcacttcggaaaaacttttgactcaatgcaacagtgtcataatcctttgcgctgtctctttgaagaaaaatcaggagtgcaagatatcgattaattccttggaagttatgatggttagtcttagtttaactctcttgaagaagggttttttcagttggtcaacactctttagaggtaaaaaaaaaaaaaaaaaaaaaaaaaagagaaatcttcatgtaatttaccatgattctacgtttttgcaagcaaaaatgaagataatccgagcgcatgcggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z