BBa_K1123017 1 BBa_K1123017 DNA Coding Sequence for EGFP Protein 2013-08-30T11:00:00Z 2015-05-08T01:09:16Z We found the DNA sequence for this part on the following website: http://www.algosome.com/resources/common-sequences.html This part contains the DNA sequence for the EGFP protein. It can be placed behind any promoter of your choice and expressed with ease. Within the scope of our project this protein was expressed as a control. It is a highly fluorescent protein and could be easily analysed to reveal the workings of your promoter or other constructs. false false _1435_ 0 16850 9 Not in stock false We did not adapt the DNA sequence in any way before using this part. false Ardjan van der Linden BBa_K1328000 1 BBa_K1328000 HXT1-CMVmini 2014-10-08T11:00:00Z 2015-05-08T01:09:54Z HXT1 promoter sequence is cloned from S. cerevisiae genome via PCR. The CMV-mini promoter is cloned from a commercial plasmid. Glucose-regulated eukaryotic promoter sequence, activated under high glucose concentrations. Works in yeast as well as human fibroblast cells. HXT1 encodes a yeast hexose transporter, we fused its promoter sequence (-750 ~ -161 bp) to a CMV-mini promoter sequence (204 bp) to ensure glucose-sensitivity as well as high expression rates. Ferrer-Mart??nez A, Riera A, Jim??nez-Chillar??n JC, Herrero P, Moreno F, G??mez-Foix AM. A glucose response element from the S. cerevisiae hexose transporter HXT1 gene is sensitive to glucose in human fibroblasts. J Mol Biol. 2004 May 7;338(4):657-67. PubMed PMID: 15099735. false false _1703_ 0 15664 9 It's complicated false Region -750~-161 bp was selected from the HXT1 promoter for maximal glucose sensitivity. A CMV-mini promoter sequence was added to increase expression rate. false Tianyi Li annotation2406691 1 CMV-mini Promoter range2406691 1 587 790 annotation2406655 1 HXT-1 Promoter range2406655 1 1 586 BBa_K1328001 1 BBa_K1328001 HXT1-CMVmini-EGFP-tADH1 2014-10-08T11:00:00Z 2015-05-08T01:09:54Z Promoter region is assembled from parts: HXT1-CMVmini promoter (BBa_K1328001), EGFP (BBa_K1123017) and tADH1 transcription terminator. This is a composite part containing a glucose-sensitive promoter (BBa_K1328000) and a EGFP reporter with a tADH1 transcription terminator. S. cerevisiae cells transformed with this part was cultured in glucose-free medium with glycerol as carbon-source for 8 hrs. They were then cultured for an additional hour with varying glucose levels (0, 5, 15, 25 mmol/L). A increasing level of GFP expression was observed with increasing levels of glucose concentration, in both microscopy and flow cytometry observations. false false _1703_ 0 15664 9 Not in stock false Sequence contains EcoRI and PstI restriction sites, consider using XbaI and SpeI for cloning. false Tianyi Li component2407190 1 BBa_K1328000 component2407191 1 BBa_K1123017 annotation2407190 1 BBa_K1328000 range2407190 1 1 790 annotation2407191 1 BBa_K1123017 range2407191 1 797 1513 BBa_K1328000_sequence 1 acgcaacttcgggtgttaagaaatattttgctacattagataatggtggagtttcctggcttgtcggataaaagccatcaaatgtcgcagcagctcatgtttacgtttgctgtcttctgcccacgtcatatgagtggtattcttctatcagcacttgatgaatattctttttctcatatatctgaaagacaaaagatcggcacggcaatgccctgctgcatttcttcctagtttttccgaatttccattacgtattggatcttgtgcgcatatttgtcagtccttcacggaaaaaaaaaaagagcactgggtcacttcggaaaaacttttgactcaatgcaacagtgtcataatcctttgcgctgtctctttgaagaaaaatcaggagtgcaagatatcgattaattccttggaagttatgatggttagtcttagtttaactctcttgaagaagggttttttcagttggtcaacactctttagaggtaaaaaaaaaaaaaaaaaaaaaaaaaagagaaatcttcatgtaatttaccatgattctacgtttttgcaagcaaaaatgaagataatccgagcgcatgcggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagct BBa_K1328001_sequence 1 acgcaacttcgggtgttaagaaatattttgctacattagataatggtggagtttcctggcttgtcggataaaagccatcaaatgtcgcagcagctcatgtttacgtttgctgtcttctgcccacgtcatatgagtggtattcttctatcagcacttgatgaatattctttttctcatatatctgaaagacaaaagatcggcacggcaatgccctgcagcatttcttcctagtttttccgaatttccattacgtattggatcttgtgcgcatatttgtcagtccttcacggaaaaaaaaaaagagcactgggtcacttcggaaaaacttttgactcaatgcaacagtgtcataatcctttgcgctgtctctttgaagaaaaatcaggagtgcaagatatcgattaattccttggaagttatgatggttagtcttagtttaactctcttgaagaagggttttttcagttggtcaacactctttagaggtaaaaaaaaaaaaaaaaaaaaaaaaaagagaattcttcatgtaatttaccatgattctacgtttttgcaagcaaaaatgaagataatccgagcgcatgcggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagcttactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaag BBa_K1123017_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z