BBa_K1328002 1 BBa_K1328002 HXT1-CMVmini-EGFP-tADH1 2014-10-08T11:00:00Z 2015-05-08T01:09:54Z Promoter region is assembled from parts: HXT1-CMVmini promoter (BBa_K1328001), EGFP (BBa_K1123017) and tADH1 transcription terminator. This is a composite part containing a glucose-sensitive promoter (BBa_K1328000) and a EGFP reporter with a tADH1 transcription terminator. S. cerevisiae cells transformed with this part was cultured in glucose-free medium with glycerol as carbon-source for 8 hrs. They were then cultured for an additional hour with varying glucose levels (0, 5, 15, 25 mmol/L). A increasing level of GFP expression was observed with increasing levels of glucose concentration, in both microscopy and flow cytometry observations. false false _1703_ 0 15664 9 It's complicated false Part sequence contains EcoRI and PstI restriction sites, consider using SpeI and XbaI for cloning. false Lingfeng Hou annotation2407293 1 tADH1 terminator range2407293 1 1561 1748 annotation2407292 1 EGFP range2407292 1 840 1556 annotation2407290 1 HXT1 Promoter range2407290 1 1 586 annotation2407291 1 CMV-mini Promoter range2407291 1 587 790 BBa_K1328002_sequence 1 acgcaacttcgggtgttaagaaatattttgctacattagataatggtggagtttcctggcttgtcggataaaagccatcaaatgtcgcagcagctcatgtttacgtttgctgtcttctgcccacgtcatatgagtggtattcttctatcagcacttgatgaatattctttttctcatatatctgaaagacaaaagatcggcacggcaatgccctgctgcatttcttcctagtttttccgaatttccattacgtattggatcttgtgcgcatatttgtcagtccttcacggaaaaaaaaaaagagcactgggtcacttcggaaaaacttttgactcaatgcaacagtgtcataatcctttgcgctgtctctttgaagaaaaatcaggagtgcaagatatcgattaattccttggaagttatgatggttagtcttagtttaactctcttgaagaagggttttttcagttggtcaacactctttagaggtaaaaaaaaaaaaaaaaaaaaaaaaaagagaaatcttcatgtaatttaccatgattctacgtttttgcaagcaaaaatgaagataatccgagcgcatgcggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctgatgtagtttagtgaaccgtcagatccgctagcgctaccggtcgccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtagcgcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccgg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z