BBa_K1329001 1 BBa_K1329001 Hag-KpnI 2014-09-30T11:00:00Z 2015-05-08T01:09:54Z The sequence for this part comes from the Bacillus subtilis genome. Hag-KpnI consists of the Bacillus subtilis flagellin gene which as designed with a KpnI site to integrate different domains, tags or modules with a Gibson assembly after linearizing the vector with KpnI. Using a shuttle vector for gam-positive bacteria it is possible to integrate the modified flagellin construct into the Bacillus genome considering a special heat shock protocoland specific flanks besides the used gene locus. By engineering new modules into the conserved structure of flagellin Bacillus is able to form flagella build up from those modified flagellins. false false _1704_ 0 22000 9 It's complicated false The position of the KpnI site had to be well placed in order to provide misfolding and structural problems of the flagellin which could influence the assembly of the filament. false Daniela Geist BBa_K1329001_sequence 1 tattcagggaggaacaaaacatgagaattaaccacaatattgcagcgcttaacacactgaaccgtttgtcttcaaacaacagtgcgagccaaaagaacatggagaaactttcttcaggtcttcgcatcaaccgtgcgggagatgacgcagcaggtcttgcgatctctgaaaaaatgagaggacaaatcagaggtcttgaaatggcttctaaaaactctcaagacggaatctctcttatccaaacagctgagggtgcattaactgaaactcatgcgatccttcaacgtgttcgtgagctagttgttcaagctggaaacactggaactcaggacaaagcaactgatttgcaatctattcaagatgaaatttcagctttaacagatgaaatcgatggtatttcaaatcgtacagagttcaatggtaagaaattgctcgatggcacttacaaagttgacacagctactcctgcaaatcaaaagaacttggtattccaaatcggagcaaatgctacacagcaaatctctgtaaatattgaggatatgggtgctgacgctcttggaattaaagaagctgatggttcaattgcagctcttcattcagttaatgatcttgacgtaacaaaattcgcagataatgcaggtaccgcagatactgctgatatcggtttcgatgctcaattgaaagttgttgatgaagcgatcaaccaagtttcttctcaacgtgctaagcttggtgcggtacaaaatcgtctggagcacacaattaacaacttaagcgcttctggtgaaaacttgacagctgctgagtctcgtatccgtgacgttgacatggctaaagagatgagcgagttcacaaagaacaacattctttctcaggcttctcaagctatgcttgctcaagcaaaccaacagccgcaaaacgtacttcaattattacgttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z