BBa_K1329002 1 BBa_K1329002 Insertion of the Metallothionein Cup-1-1 domain into Bacillus subtilis flagellin 2014-10-06T11:00:00Z 2015-05-08T01:09:54Z Bacillus subtilis Saccharomyces cerevisiae The bacterial flagellum is built up of multiple copies of a protein called flagellin. The structure of this protein is mainly conserved between different species, but the variation of a loop directed to the outside of the flagellum can be observed. As we aim to fish metal ions from solution the bacterial flagellum was chosen as a protein scaffold system. We inserted the metallothionein Cup1-1 from Saccharomyces cerevisiae into the variable loop of B. subtilis flagellin. Cup1-1 can bind up to 7 ions of either copper or silver. false false _1704_ 0 22000 9 It's complicated false GGSS-linkers for better exposition to the outside of the flagellin false Daniela Geist BBa_K1329002_sequence 1 tattcagggaggaacaaaacatgagaattaaccacaatattgcagcgcttaacacactgaaccgtttgtcttcaaacaacagtgcgagccaaaagaacatggagaaactttcttcaggtcttcgcatcaaccgtgcgggagatgacgcagcaggtcttgcgatctctgaaaaaatgagaggacaaatcagaggtcttgaaatggcttctaaaaactctcaagacggaatctctcttatccaaacagctgagggtgcattaactgaaactcatgcgatccttcaacgtgttcgtgagctagttgttcaagctggaaacactggaactcaggacaaagcaactgatttgcaatctattcaagatgaaatttcagctttaacagatgaaatcgatggtatttcaaatcgtacagagttcaatggtaagaaattgctcgatggcacttacaaagttgacacagctactcctgcaaatcaaaagaacttggtattccaaatcggagcaaatgctacacagcaaatctctgtaaatattgaggatatgggtgctgacgctcttggaattaaagaagctgatggttcaattgcagctcttcattcagttaatgatcttgacgtaacaaaattcgcagataatgcaggctcacatgagtgccaatgccaatgtggtagctgcaaaaataatgaacaatgccaaaaatcatgtagctgcccaacggggtgtaacagcgacgacaaatgcccctgcggtaacggctcagcagatactgctgatatcggtttcgatgctcaattgaaagttgttgatgaagcgatcaaccaagtttcttctcaacgtgctaagcttggtgcggtacaaaatcgtctggagcacacaattaacaacttaagcgcttctggtgaaaacttgacagctgctgagtctcgtatccgtgacgttgacatggctaaagagatgagcgagttcacaaagaacaacattctttctcaggcttctcaagctatgcttgctcaagcaaaccaacagccgcaaaacgtacttcaattattacgttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z