BBa_K133036 1 RGD-His-St RGD-His affinity tag-Stop 2008-10-25T11:00:00Z 2015-05-08T01:09:55Z Part was constructed by primer annealing. This is His-tag with stop codon and RGD seguence. His-tag is used for detection of His-tagged proteins by anti-His antibodies. RGD is arginine-glycine-aspartic amino acid sequence, present on several extracellular matrix proteins. It is required for binding to integrins. false false _231_ 0 3350 9 It's complicated false Cloned into pSB1AK3 vector. false Jerneja Mori BBa_K133010 1 BBa_K133010 CF213 2008-10-29T12:00:00Z 2015-05-08T01:09:55Z Genomic DNA Chimeric flagellin false false _231_ 0 3351 9 It's complicated false None false Vid Kocar BBa_K133132 1 linker 8 aa protein domain linker 2008-10-23T11:00:00Z 2015-05-08T01:09:57Z false false _231_ 0 3358 9 It's complicated false false Jan Lonzaric BBa_K133071 1 BBa_K133071 UreB 2008-10-21T11:00:00Z 2015-05-08T01:09:56Z Cloned into pSB1AK3 vector. false false _231_ 0 1883 9 It's complicated false false Katja Kolar BBa_K133046 1 BBa_K133046 CF215 2008-10-29T12:00:00Z 2015-05-08T01:09:55Z We used polimerase chain reaction from chimeric flagellin (BBa_K133016). This is a part that is composed of C-terminal end of chimeric flagellin (BBa_K133016). We used aminoacids from 215th aminoacid on. Chimeric flagellin is composed of C- and N-terminal part of E.coli flagellin and of variable region of H.pylori. The E.coli parts are responsible for binding and activation of cell membrane receptor TLR5 to initiate immune response, while H.pylori variable region was used to array the immune response against H.pylori. This part was used in combination with CF213 - chimeric flagellin (BBa_K133010). Between the CF213 and CF215 we have put H.pylori antigens in order to array the immune response against H.pylori. false false _231_ 0 3352 9 It's complicated false It was important to split the chimeric flagellin in the variable region to assure that the antigen will be in the right place and not to interrupt the 3d structure of the protein. false Ana Lasic BBa_K133012 1 BBa_K133012 CF213-UreB-linker-CF215-RGD-Histop 2008-10-29T12:00:00Z 2015-05-08T01:09:55Z plasmid CF213-UreB-LINKER-CF215-RGD-HIS-STOP false false _231_ 0 3352 9 It's complicated false none false Ana Lasic component1996069 1 BBa_K133010 component1996071 1 BBa_K133132 component1996070 1 BBa_K133071 component1996073 1 BBa_K133036 component1996072 1 BBa_K133046 annotation1996069 1 BBa_K133010 range1996069 1 1 636 annotation1996072 1 BBa_K133046 range1996072 1 2388 3296 annotation1996070 1 BBa_K133071 range1996070 1 643 2349 annotation1996071 1 BBa_K133132 range1996071 1 2358 2381 annotation1996073 1 BBa_K133036 range1996073 1 3305 3334 BBa_K133010_sequence 1 atggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcgtctgtcttctggcttgcgtattaacagcgcgaaggatgacgcagcgggtcaggcgattgctaaccgtttcacctctaacattaaaggcctgactcaggcggcccgtaacgccaacgacggtatctccgttgcgcagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtgtgcgtgaactgacggtacaggccactaccggtactaactctgagtctgatctgtcttctatccaggacgaaattaaatcccgtctggatgaaattgaccgcgtatctggtcagacccagttcaacggcgtgaacgtgctggcaaaaaatggctccatgaaaatccaggttggcgcaaatgataaccagactatcactatcgatctgaagcagattgatgctaaaactcttggccttgatggttttagcgttaaagcgttaatcacggcttctggggatattagcttgacttttaaacaagtggatggcgtgaatgatgtaactttagagagcgtaaaagtttctagttcagcaggtacaggg BBa_K133012_sequence 1 atggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcgtctgtcttctggcttgcgtattaacagcgcgaaggatgacgcagcgggtcaggcgattgctaaccgtttcacctctaacattaaaggcctgactcaggcggcccgtaacgccaacgacggtatctccgttgcgcagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtgtgcgtgaactgacggtacaggccactaccggtactaactctgagtctgatctgtcttctatccaggacgaaattaaatcccgtctggatgaaattgaccgcgtatctggtcagacccagttcaacggcgtgaacgtgctggcaaaaaatggctccatgaaaatccaggttggcgcaaatgataaccagactatcactatcgatctgaagcagattgatgctaaaactcttggccttgatggttttagcgttaaagcgttaatcacggcttctggggatattagcttgacttttaaacaagtggatggcgtgaatgatgtaactttagagagcgtaaaagtttctagttcagcaggtacagggtactagatgaaaaagattagcagaaaagaatatgtttctatgtatggccctactacaggcgataaagtgagattgggcgatacagacttgatcgctgaagtagaacatgactacaccatttatggcgaagagcttaaattcggtggcggtaaaaccctaagagaaggcatgagccaatctaacaatcctagcaaagaagaactggatttaatcatcactaacgctttaatcgtggattacaccggtatttataaagcggatattggtattaaagatggcaaaatcgctggcattggtaaaggcggtaacaaagacatgcaagatggcgttaaaaacaatcttagcgtgggtcctgctactgaagccttagccggtgaaggtttgatcgtaactgctggtggtattgacacacacatccacttcatctccccccaacaaatccctacagcttttgcaagcggtgtaacaaccatgattggtggcggaactggccctgctgatggcactaacgcaaccactatcactccaggcagaagaaatttaaaattcatgctcagagcggctgaagaatattctatgaactttggtttcttggctaaaggtaacgtttctaacgatgcgagcttagccgatcaaattgaagctggtgcgattggctttaaaatccacgaagactggggtaccactccttctgcaatcaatcatgcgttagatgttgcagacaaatacgatgtgcaagtcgctatccacacagacactttgaatgaagccggttgcgtggaagacactatggcagccattgccggacgcactatgcacactttccacactgaaggcgctggcggcggacacgctcctgatattattaaagtggccggtgaacacaacattctacctgcttccactaaccccactatccctttcaccgtgaatacagaagccgaacacatggacatgcttatggtgtgccaccacttggataaaagcattaaagaagatgttcagttcgctgattcaaggatccgccctcaaaccattgcggctgaagacactttgcatgacatggggattttctcaatcaccagttctgactctcaagctatgggtcgtgtgggtgaagttatcaccagaacttggcaaacagctgacaaaaacaaaaaagaatttggccgcttgaaagaagaaaaaggcgataacgacaacttcaggatcaaacgctacttgtctaaatacaccattaacccagcgatcgctcatgggattagcgagtatgtcggttctgtagaagtgggcaaagtggctgacttggtattgtggagtccagcattctttggtgtgaaacccaacatgatcatcaaaggcggattcattgcattgagtcaaatgggtgatgcgaacgcttctatccctaccccacaaccggtttattacagagaaatgttcgctcatcatggtaaagctaaatacgatgcaaacatcacttttgtgtctcaagcggcttatgacaaaggcattaaagaagaattagggcttgaaagacaagtgttgccggtaaaaaattgcagaaacatcactaaaaaagacatgcaattcaacgacactaccgctcacattgaagtcaatcctgaaacttaccatgtgttcgtggatggcaaagaagtaacttctaaaccagccaataaagtgagcttggcgcaactctttagcattttctactagagtccgcttgttactgtgagctttcctactagatgggtgtgttggcagaagtgattaacaaaaattctaaccgaacaggggttaaagcttatgcgagcgttatcaccacgagcgatgtggcggtccaatcaggaagtttgagtaatttaactttaaatgggatccatttgggtaatatcgcagatattaagaaaaatgactcagacggaaggttagtcgcagcgatcaatgcggttacttcagaaaccggcgtggaagcttatacggatcaaaaagggcgcttgaatttgcgcagtatagatggtcgtgggattgaaatcaaaaccgatagcgtcagtaatgggcctagtgctttaacgatggtcaatggcggtcaggatttaacaaaaggttctactaactatgggaggctttctctcacacgcttagacgctaaaagcatcaatgtcgtttcggcttctgattcgcaacatttaggtttcacagcgattggttttggggaatctcaagtggcagaaaccacggtgaatttgcgcgatgttactgggaattttaacgctaatgtcaaatcagccagtggcgcgaactataacgccgtgatcgctagcggtaaccaaagcttgggatctggggttacaaccttaagagctgttgcaaatggtaaaaccacggatccgctgaaagcgctggacgatgctatcgcatctgtagacaaattccgttcttccctcggtgcggtgcaaaaccgtctggattccgcggttactaacctgaacaacaccactaccaacctgtctgaagcgcagtcccgtattcaggacgccgactatgcgaccgaagtgtccaatatgtcgaaagcgcagatcatccagcaggccggtaactccgtgttggcaaaagctaaccaggtaccgcagcaggttctgtctctgttacagggttactagagcgaggagaccaccaccaccaccaccactag BBa_K133071_sequence 1 atgaaaaagattagcagaaaagaatatgtttctatgtatggccctactacaggcgataaagtgagattgggcgatacagacttgatcgctgaagtagaacatgactacaccatttatggcgaagagcttaaattcggtggcggtaaaaccctaagagaaggcatgagccaatctaacaatcctagcaaagaagaactggatttaatcatcactaacgctttaatcgtggattacaccggtatttataaagcggatattggtattaaagatggcaaaatcgctggcattggtaaaggcggtaacaaagacatgcaagatggcgttaaaaacaatcttagcgtgggtcctgctactgaagccttagccggtgaaggtttgatcgtaactgctggtggtattgacacacacatccacttcatctccccccaacaaatccctacagcttttgcaagcggtgtaacaaccatgattggtggcggaactggccctgctgatggcactaacgcaaccactatcactccaggcagaagaaatttaaaattcatgctcagagcggctgaagaatattctatgaactttggtttcttggctaaaggtaacgtttctaacgatgcgagcttagccgatcaaattgaagctggtgcgattggctttaaaatccacgaagactggggtaccactccttctgcaatcaatcatgcgttagatgttgcagacaaatacgatgtgcaagtcgctatccacacagacactttgaatgaagccggttgcgtggaagacactatggcagccattgccggacgcactatgcacactttccacactgaaggcgctggcggcggacacgctcctgatattattaaagtggccggtgaacacaacattctacctgcttccactaaccccactatccctttcaccgtgaatacagaagccgaacacatggacatgcttatggtgtgccaccacttggataaaagcattaaagaagatgttcagttcgctgattcaaggatccgccctcaaaccattgcggctgaagacactttgcatgacatggggattttctcaatcaccagttctgactctcaagctatgggtcgtgtgggtgaagttatcaccagaacttggcaaacagctgacaaaaacaaaaaagaatttggccgcttgaaagaagaaaaaggcgataacgacaacttcaggatcaaacgctacttgtctaaatacaccattaacccagcgatcgctcatgggattagcgagtatgtcggttctgtagaagtgggcaaagtggctgacttggtattgtggagtccagcattctttggtgtgaaacccaacatgatcatcaaaggcggattcattgcattgagtcaaatgggtgatgcgaacgcttctatccctaccccacaaccggtttattacagagaaatgttcgctcatcatggtaaagctaaatacgatgcaaacatcacttttgtgtctcaagcggcttatgacaaaggcattaaagaagaattagggcttgaaagacaagtgttgccggtaaaaaattgcagaaacatcactaaaaaagacatgcaattcaacgacactaccgctcacattgaagtcaatcctgaaacttaccatgtgttcgtggatggcaaagaagtaacttctaaaccagccaataaagtgagcttggcgcaactctttagcattttc BBa_K133046_sequence 1 atgggtgtgttggcagaagtgattaacaaaaattctaaccgaacaggggttaaagcttatgcgagcgttatcaccacgagcgatgtggcggtccaatcaggaagtttgagtaatttaactttaaatgggatccatttgggtaatatcgcagatattaagaaaaatgactcagacggaaggttagtcgcagcgatcaatgcggttacttcagaaaccggcgtggaagcttatacggatcaaaaagggcgcttgaatttgcgcagtatagatggtcgtgggattgaaatcaaaaccgatagcgtcagtaatgggcctagtgctttaacgatggtcaatggcggtcaggatttaacaaaaggttctactaactatgggaggctttctctcacacgcttagacgctaaaagcatcaatgtcgtttcggcttctgattcgcaacatttaggtttcacagcgattggttttggggaatctcaagtggcagaaaccacggtgaatttgcgcgatgttactgggaattttaacgctaatgtcaaatcagccagtggcgcgaactataacgccgtgatcgctagcggtaaccaaagcttgggatctggggttacaaccttaagagctgttgcaaatggtaaaaccacggatccgctgaaagcgctggacgatgctatcgcatctgtagacaaattccgttcttccctcggtgcggtgcaaaaccgtctggattccgcggttactaacctgaacaacaccactaccaacctgtctgaagcgcagtcccgtattcaggacgccgactatgcgaccgaagtgtccaatatgtcgaaagcgcagatcatccagcaggccggtaactccgtgttggcaaaagctaaccaggtaccgcagcaggttctgtctctgttacagggt BBa_K133132_sequence 1 tccgcttgttactgtgagctttcc BBa_K133036_sequence 1 cgaggagaccaccaccaccaccaccactag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z