BBa_K133015 1 BBa_K133015 CF215-mCherry-RGD-Histop 2008-10-29T12:00:00Z 2015-05-08T01:09:55Z plasmid CF215-Cherry-RGD-HIS-STOP false false _231_ 0 3352 9 It's complicated false none false Ana Lasic component1996982 1 BBa_K133036 component1996981 1 BBa_K133055 component1996980 1 BBa_K133046 annotation1996981 1 BBa_K133055 range1996981 1 918 1625 annotation1996982 1 BBa_K133036 range1996982 1 1634 1663 annotation1996980 1 BBa_K133046 range1996980 1 1 909 BBa_K133036 1 RGD-His-St RGD-His affinity tag-Stop 2008-10-25T11:00:00Z 2015-05-08T01:09:55Z Part was constructed by primer annealing. This is His-tag with stop codon and RGD seguence. His-tag is used for detection of His-tagged proteins by anti-His antibodies. RGD is arginine-glycine-aspartic amino acid sequence, present on several extracellular matrix proteins. It is required for binding to integrins. false false _231_ 0 3350 9 It's complicated false Cloned into pSB1AK3 vector. false Jerneja Mori BBa_K133055 1 BBa_K133055 mCherry 2008-10-29T12:00:00Z 2015-05-08T01:09:56Z we got it from mCherry is a red fluorescent protein used for analysis of localisation of protein. false false _231_ 0 3352 9 It's complicated false none false Ana Lasic BBa_K133046 1 BBa_K133046 CF215 2008-10-29T12:00:00Z 2015-05-08T01:09:55Z We used polimerase chain reaction from chimeric flagellin (BBa_K133016). This is a part that is composed of C-terminal end of chimeric flagellin (BBa_K133016). We used aminoacids from 215th aminoacid on. Chimeric flagellin is composed of C- and N-terminal part of E.coli flagellin and of variable region of H.pylori. The E.coli parts are responsible for binding and activation of cell membrane receptor TLR5 to initiate immune response, while H.pylori variable region was used to array the immune response against H.pylori. This part was used in combination with CF213 - chimeric flagellin (BBa_K133010). Between the CF213 and CF215 we have put H.pylori antigens in order to array the immune response against H.pylori. false false _231_ 0 3352 9 It's complicated false It was important to split the chimeric flagellin in the variable region to assure that the antigen will be in the right place and not to interrupt the 3d structure of the protein. false Ana Lasic BBa_K133015_sequence 1 atgggtgtgttggcagaagtgattaacaaaaattctaaccgaacaggggttaaagcttatgcgagcgttatcaccacgagcgatgtggcggtccaatcaggaagtttgagtaatttaactttaaatgggatccatttgggtaatatcgcagatattaagaaaaatgactcagacggaaggttagtcgcagcgatcaatgcggttacttcagaaaccggcgtggaagcttatacggatcaaaaagggcgcttgaatttgcgcagtatagatggtcgtgggattgaaatcaaaaccgatagcgtcagtaatgggcctagtgctttaacgatggtcaatggcggtcaggatttaacaaaaggttctactaactatgggaggctttctctcacacgcttagacgctaaaagcatcaatgtcgtttcggcttctgattcgcaacatttaggtttcacagcgattggttttggggaatctcaagtggcagaaaccacggtgaatttgcgcgatgttactgggaattttaacgctaatgtcaaatcagccagtggcgcgaactataacgccgtgatcgctagcggtaaccaaagcttgggatctggggttacaaccttaagagctgttgcaaatggtaaaaccacggatccgctgaaagcgctggacgatgctatcgcatctgtagacaaattccgttcttccctcggtgcggtgcaaaaccgtctggattccgcggttactaacctgaacaacaccactaccaacctgtctgaagcgcagtcccgtattcaggacgccgactatgcgaccgaagtgtccaatatgtcgaaagcgcagatcatccagcaggccggtaactccgtgttggcaaaagctaaccaggtaccgcagcaggttctgtctctgttacagggttactagagataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctccaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggatccaaaaaagaagagaaaggtactagagcgaggagaccaccaccaccaccaccactag BBa_K133046_sequence 1 atgggtgtgttggcagaagtgattaacaaaaattctaaccgaacaggggttaaagcttatgcgagcgttatcaccacgagcgatgtggcggtccaatcaggaagtttgagtaatttaactttaaatgggatccatttgggtaatatcgcagatattaagaaaaatgactcagacggaaggttagtcgcagcgatcaatgcggttacttcagaaaccggcgtggaagcttatacggatcaaaaagggcgcttgaatttgcgcagtatagatggtcgtgggattgaaatcaaaaccgatagcgtcagtaatgggcctagtgctttaacgatggtcaatggcggtcaggatttaacaaaaggttctactaactatgggaggctttctctcacacgcttagacgctaaaagcatcaatgtcgtttcggcttctgattcgcaacatttaggtttcacagcgattggttttggggaatctcaagtggcagaaaccacggtgaatttgcgcgatgttactgggaattttaacgctaatgtcaaatcagccagtggcgcgaactataacgccgtgatcgctagcggtaaccaaagcttgggatctggggttacaaccttaagagctgttgcaaatggtaaaaccacggatccgctgaaagcgctggacgatgctatcgcatctgtagacaaattccgttcttccctcggtgcggtgcaaaaccgtctggattccgcggttactaacctgaacaacaccactaccaacctgtctgaagcgcagtcccgtattcaggacgccgactatgcgaccgaagtgtccaatatgtcgaaagcgcagatcatccagcaggccggtaactccgtgttggcaaaagctaaccaggtaccgcagcaggttctgtctctgttacagggt BBa_K133036_sequence 1 cgaggagaccaccaccaccaccaccactag BBa_K133055_sequence 1 ataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctccaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggatccaaaaaagaagagaaagg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z