BBa_K133036
1
RGD-His-St
RGD-His affinity tag-Stop
2008-10-25T11:00:00Z
2015-05-08T01:09:55Z
Part was constructed by primer annealing.
This is His-tag with stop codon and RGD seguence. His-tag is used for detection of His-tagged proteins by anti-His antibodies. RGD is arginine-glycine-aspartic amino acid sequence, present on several extracellular matrix proteins. It is required for binding to integrins.
false
false
_231_
0
3350
9
It's complicated
false
Cloned into pSB1AK3 vector.
false
Jerneja Mori
BBa_K133018
1
BBa_K133018
CMV-CF-UreB-RGD-Histop
2008-10-29T12:00:00Z
2015-05-08T01:09:55Z
plasmid
CMV-CF-UreB-RGD-HIS-STOP
false
false
_231_
0
3352
9
It's complicated
false
none
false
Ana Lasic
component1996081
1
BBa_K133071
component1996080
1
BBa_K133016
component1996079
1
BBa_I712004
component1996082
1
BBa_K133036
annotation1996082
1
BBa_K133036
range1996082
1
3927
3956
annotation1996081
1
BBa_K133071
range1996081
1
2212
3918
annotation1996079
1
BBa_I712004
range1996079
1
1
654
annotation1996080
1
BBa_K133016
range1996080
1
661
2205
BBa_K133016
1
BBa_K133016
CF
2008-10-29T12:00:00Z
2015-05-08T01:09:55Z
Genomic DNA
Whole chimeric protein
false
false
_231_
0
3351
9
It's complicated
false
None
false
Vid Kocar
BBa_I712004
1
BBa_I712004
CMV promoter
2007-10-18T11:00:00Z
2015-08-31T04:07:45Z
pcDNA3 by Invitrogen
a constitutive expression promoter or use in mammalian cells. Ribosome binding site is included.
false
false
_130_
0
1896
9
It's complicated
false
Cloned into pSB1AC3 vector.
false
Andrej Ondracka
BBa_K133071
1
BBa_K133071
UreB
2008-10-21T11:00:00Z
2015-05-08T01:09:56Z
Cloned into pSB1AK3 vector.
false
false
_231_
0
1883
9
It's complicated
false
false
Katja Kolar
BBa_K133071_sequence
1
atgaaaaagattagcagaaaagaatatgtttctatgtatggccctactacaggcgataaagtgagattgggcgatacagacttgatcgctgaagtagaacatgactacaccatttatggcgaagagcttaaattcggtggcggtaaaaccctaagagaaggcatgagccaatctaacaatcctagcaaagaagaactggatttaatcatcactaacgctttaatcgtggattacaccggtatttataaagcggatattggtattaaagatggcaaaatcgctggcattggtaaaggcggtaacaaagacatgcaagatggcgttaaaaacaatcttagcgtgggtcctgctactgaagccttagccggtgaaggtttgatcgtaactgctggtggtattgacacacacatccacttcatctccccccaacaaatccctacagcttttgcaagcggtgtaacaaccatgattggtggcggaactggccctgctgatggcactaacgcaaccactatcactccaggcagaagaaatttaaaattcatgctcagagcggctgaagaatattctatgaactttggtttcttggctaaaggtaacgtttctaacgatgcgagcttagccgatcaaattgaagctggtgcgattggctttaaaatccacgaagactggggtaccactccttctgcaatcaatcatgcgttagatgttgcagacaaatacgatgtgcaagtcgctatccacacagacactttgaatgaagccggttgcgtggaagacactatggcagccattgccggacgcactatgcacactttccacactgaaggcgctggcggcggacacgctcctgatattattaaagtggccggtgaacacaacattctacctgcttccactaaccccactatccctttcaccgtgaatacagaagccgaacacatggacatgcttatggtgtgccaccacttggataaaagcattaaagaagatgttcagttcgctgattcaaggatccgccctcaaaccattgcggctgaagacactttgcatgacatggggattttctcaatcaccagttctgactctcaagctatgggtcgtgtgggtgaagttatcaccagaacttggcaaacagctgacaaaaacaaaaaagaatttggccgcttgaaagaagaaaaaggcgataacgacaacttcaggatcaaacgctacttgtctaaatacaccattaacccagcgatcgctcatgggattagcgagtatgtcggttctgtagaagtgggcaaagtggctgacttggtattgtggagtccagcattctttggtgtgaaacccaacatgatcatcaaaggcggattcattgcattgagtcaaatgggtgatgcgaacgcttctatccctaccccacaaccggtttattacagagaaatgttcgctcatcatggtaaagctaaatacgatgcaaacatcacttttgtgtctcaagcggcttatgacaaaggcattaaagaagaattagggcttgaaagacaagtgttgccggtaaaaaattgcagaaacatcactaaaaaagacatgcaattcaacgacactaccgctcacattgaagtcaatcctgaaacttaccatgtgttcgtggatggcaaagaagtaacttctaaaccagccaataaagtgagcttggcgcaactctttagcattttc
BBa_K133016_sequence
1
atggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcgtctgtcttctggcttgcgtattaacagcgcgaaggatgacgcagcgggtcaggcgattgctaaccgtttcacctctaacattaaaggcctgactcaggcggcccgtaacgccaacgacggtatctccgttgcgcagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtgtgcgtgaactgacggtacaggccactaccggtactaactctgagtctgatctgtcttctatccaggacgaaattaaatcccgtctggatgaaattgaccgcgtatctggtcagacccagttcaacggcgtgaacgtgctggcaaaaaatggctccatgaaaatccaggttggcgcaaatgataaccagactatcactatcgatctgaagcagattgatgctaaaactcttggccttgatggttttagcgttaaagcgttaatcacggcttctggggatattagcttgacttttaaacaagtggatggcgtgaatgatgtaactttagagagcgtaaaagtttctagttcagcaggcacggggatcggtgtgttagcggaagtgattaacaaaaattctaaccgaacaggggttaaagcttatgcgagcgttatcaccacgagcgatgtggcggtccaatcaggaagtttgagtaatttaactttaaatgggatccatttgggtaatatcgcagatattaagaaaaatgactcagacggaaggttagtcgcagcgatcaatgcggttacttcagaaaccggcgtggaagcttatacggatcaaaaagggcgcttgaatttgcgcagtatagatggtcgtgggattgaaatcaaaaccgatagcgtcagtaatgggcctagtgctttaacgatggtcaatggcggtcaggatttaacaaaaggttctactaactatgggaggctttctctcacacgcttagacgctaaaagcatcaatgtcgtttcggcttctgattcgcaacatttaggtttcacagcgattggttttggggaatctcaagtggcagaaaccacggtgaatttgcgcgatgttactgggaattttaacgctaatgtcaaatcagccagtggcgcgaactataacgccgtgatcgctagcggtaaccaaagcttgggatctggggttacaaccttaagagctgttgcaaatggtaaaaccacggatccgctgaaagcgctggacgatgctatcgcatctgtagacaaattccgttcttccctcggtgcggtgcaaaaccgtctggattccgcggttaccaacctgaacaacaccactaccaacctgtctgaagcgcagtcccgtattcaggacgccgactatgcgaccgaagtgtccaatatgtcgaaagcgcagatcatccagcaggccggtaactccgtgttggcaaaagctaaccaggtaccgcagcaggttctgtctctgttacagggt
BBa_K133018_sequence
1
cgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaattactagatggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcgtctgtcttctggcttgcgtattaacagcgcgaaggatgacgcagcgggtcaggcgattgctaaccgtttcacctctaacattaaaggcctgactcaggcggcccgtaacgccaacgacggtatctccgttgcgcagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtgtgcgtgaactgacggtacaggccactaccggtactaactctgagtctgatctgtcttctatccaggacgaaattaaatcccgtctggatgaaattgaccgcgtatctggtcagacccagttcaacggcgtgaacgtgctggcaaaaaatggctccatgaaaatccaggttggcgcaaatgataaccagactatcactatcgatctgaagcagattgatgctaaaactcttggccttgatggttttagcgttaaagcgttaatcacggcttctggggatattagcttgacttttaaacaagtggatggcgtgaatgatgtaactttagagagcgtaaaagtttctagttcagcaggcacggggatcggtgtgttagcggaagtgattaacaaaaattctaaccgaacaggggttaaagcttatgcgagcgttatcaccacgagcgatgtggcggtccaatcaggaagtttgagtaatttaactttaaatgggatccatttgggtaatatcgcagatattaagaaaaatgactcagacggaaggttagtcgcagcgatcaatgcggttacttcagaaaccggcgtggaagcttatacggatcaaaaagggcgcttgaatttgcgcagtatagatggtcgtgggattgaaatcaaaaccgatagcgtcagtaatgggcctagtgctttaacgatggtcaatggcggtcaggatttaacaaaaggttctactaactatgggaggctttctctcacacgcttagacgctaaaagcatcaatgtcgtttcggcttctgattcgcaacatttaggtttcacagcgattggttttggggaatctcaagtggcagaaaccacggtgaatttgcgcgatgttactgggaattttaacgctaatgtcaaatcagccagtggcgcgaactataacgccgtgatcgctagcggtaaccaaagcttgggatctggggttacaaccttaagagctgttgcaaatggtaaaaccacggatccgctgaaagcgctggacgatgctatcgcatctgtagacaaattccgttcttccctcggtgcggtgcaaaaccgtctggattccgcggttaccaacctgaacaacaccactaccaacctgtctgaagcgcagtcccgtattcaggacgccgactatgcgaccgaagtgtccaatatgtcgaaagcgcagatcatccagcaggccggtaactccgtgttggcaaaagctaaccaggtaccgcagcaggttctgtctctgttacagggttactagatgaaaaagattagcagaaaagaatatgtttctatgtatggccctactacaggcgataaagtgagattgggcgatacagacttgatcgctgaagtagaacatgactacaccatttatggcgaagagcttaaattcggtggcggtaaaaccctaagagaaggcatgagccaatctaacaatcctagcaaagaagaactggatttaatcatcactaacgctttaatcgtggattacaccggtatttataaagcggatattggtattaaagatggcaaaatcgctggcattggtaaaggcggtaacaaagacatgcaagatggcgttaaaaacaatcttagcgtgggtcctgctactgaagccttagccggtgaaggtttgatcgtaactgctggtggtattgacacacacatccacttcatctccccccaacaaatccctacagcttttgcaagcggtgtaacaaccatgattggtggcggaactggccctgctgatggcactaacgcaaccactatcactccaggcagaagaaatttaaaattcatgctcagagcggctgaagaatattctatgaactttggtttcttggctaaaggtaacgtttctaacgatgcgagcttagccgatcaaattgaagctggtgcgattggctttaaaatccacgaagactggggtaccactccttctgcaatcaatcatgcgttagatgttgcagacaaatacgatgtgcaagtcgctatccacacagacactttgaatgaagccggttgcgtggaagacactatggcagccattgccggacgcactatgcacactttccacactgaaggcgctggcggcggacacgctcctgatattattaaagtggccggtgaacacaacattctacctgcttccactaaccccactatccctttcaccgtgaatacagaagccgaacacatggacatgcttatggtgtgccaccacttggataaaagcattaaagaagatgttcagttcgctgattcaaggatccgccctcaaaccattgcggctgaagacactttgcatgacatggggattttctcaatcaccagttctgactctcaagctatgggtcgtgtgggtgaagttatcaccagaacttggcaaacagctgacaaaaacaaaaaagaatttggccgcttgaaagaagaaaaaggcgataacgacaacttcaggatcaaacgctacttgtctaaatacaccattaacccagcgatcgctcatgggattagcgagtatgtcggttctgtagaagtgggcaaagtggctgacttggtattgtggagtccagcattctttggtgtgaaacccaacatgatcatcaaaggcggattcattgcattgagtcaaatgggtgatgcgaacgcttctatccctaccccacaaccggtttattacagagaaatgttcgctcatcatggtaaagctaaatacgatgcaaacatcacttttgtgtctcaagcggcttatgacaaaggcattaaagaagaattagggcttgaaagacaagtgttgccggtaaaaaattgcagaaacatcactaaaaaagacatgcaattcaacgacactaccgctcacattgaagtcaatcctgaaacttaccatgtgttcgtggatggcaaagaagtaacttctaaaccagccaataaagtgagcttggcgcaactctttagcattttctactagagcgaggagaccaccaccaccaccaccactag
BBa_I712004_sequence
1
cgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaat
BBa_K133036_sequence
1
cgaggagaccaccaccaccaccaccactag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z