BBa_K133036 1 RGD-His-St RGD-His affinity tag-Stop 2008-10-25T11:00:00Z 2015-05-08T01:09:55Z Part was constructed by primer annealing. This is His-tag with stop codon and RGD seguence. His-tag is used for detection of His-tagged proteins by anti-His antibodies. RGD is arginine-glycine-aspartic amino acid sequence, present on several extracellular matrix proteins. It is required for binding to integrins. false false _231_ 0 3350 9 It's complicated false Cloned into pSB1AK3 vector. false Jerneja Mori BBa_K133010 1 BBa_K133010 CF213 2008-10-29T12:00:00Z 2015-05-08T01:09:55Z Genomic DNA Chimeric flagellin false false _231_ 0 3351 9 It's complicated false None false Vid Kocar BBa_K133023 1 BBa_K133023 CMV-ss-CF213-multiHP-CF215-RGD-Histop (term) 2008-10-29T12:00:00Z 2015-05-08T01:09:55Z false true _231_ 0 3352 9 It's complicated true true Ana Lasic component1996180 1 BBa_K133010 component1996178 1 BBa_I712004 component1996179 1 BBa_I712009 component1996181 1 BBa_K133139 component1996183 1 BBa_K133036 component1996182 1 BBa_K133046 annotation1996178 1 BBa_I712004 range1996178 1 1 654 annotation1996180 1 BBa_K133010 range1996180 1 742 1377 annotation1996182 1 BBa_K133046 range1996182 1 1917 2825 annotation1996181 1 BBa_K133139 range1996181 1 1386 1910 annotation1996183 1 BBa_K133036 range1996183 1 2834 2863 annotation1996179 1 BBa_I712009 range1996179 1 661 735 BBa_I712004 1 BBa_I712004 CMV promoter 2007-10-18T11:00:00Z 2015-08-31T04:07:45Z pcDNA3 by Invitrogen a constitutive expression promoter or use in mammalian cells. Ribosome binding site is included. false false _130_ 0 1896 9 It's complicated false Cloned into pSB1AC3 vector. false Andrej Ondracka BBa_K133139 1 BBa_K133139 multiHP 2008-10-24T11:00:00Z 2015-05-08T01:09:57Z false false _231_ 0 1883 9 Not in stock false false Katja Kolar BBa_I712009 1 CD4 signal CD4 signal peptide; localizes to plasma membrane 2007-10-21T11:00:00Z 2015-08-31T04:07:45Z It comes from human gene for CD4 receptor. Coding region for first 25 amino acids (including start codon) of CD4 receptor which is signal peptide for plasma membrane localization. false false _130_ 0 1850 9 In stock false No considerations. true Peter Cimermancic BBa_K133046 1 BBa_K133046 CF215 2008-10-29T12:00:00Z 2015-05-08T01:09:55Z We used polimerase chain reaction from chimeric flagellin (BBa_K133016). This is a part that is composed of C-terminal end of chimeric flagellin (BBa_K133016). We used aminoacids from 215th aminoacid on. Chimeric flagellin is composed of C- and N-terminal part of E.coli flagellin and of variable region of H.pylori. The E.coli parts are responsible for binding and activation of cell membrane receptor TLR5 to initiate immune response, while H.pylori variable region was used to array the immune response against H.pylori. This part was used in combination with CF213 - chimeric flagellin (BBa_K133010). Between the CF213 and CF215 we have put H.pylori antigens in order to array the immune response against H.pylori. false false _231_ 0 3352 9 It's complicated false It was important to split the chimeric flagellin in the variable region to assure that the antigen will be in the right place and not to interrupt the 3d structure of the protein. false Ana Lasic BBa_I712009_sequence 1 atgaaccggggagtcccttttaggcacttgcttctggtgctgcaactggcgctcctcccagcagccactcaggga BBa_K133010_sequence 1 atggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcgtctgtcttctggcttgcgtattaacagcgcgaaggatgacgcagcgggtcaggcgattgctaaccgtttcacctctaacattaaaggcctgactcaggcggcccgtaacgccaacgacggtatctccgttgcgcagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtgtgcgtgaactgacggtacaggccactaccggtactaactctgagtctgatctgtcttctatccaggacgaaattaaatcccgtctggatgaaattgaccgcgtatctggtcagacccagttcaacggcgtgaacgtgctggcaaaaaatggctccatgaaaatccaggttggcgcaaatgataaccagactatcactatcgatctgaagcagattgatgctaaaactcttggccttgatggttttagcgttaaagcgttaatcacggcttctggggatattagcttgacttttaaacaagtggatggcgtgaatgatgtaactttagagagcgtaaaagtttctagttcagcaggtacaggg BBa_K133023_sequence 1 cgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaattactagatgaaccggggagtcccttttaggcacttgcttctggtgctgcaactggcgctcctcccagcagccactcagggatactagatggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcgtctgtcttctggcttgcgtattaacagcgcgaaggatgacgcagcgggtcaggcgattgctaaccgtttcacctctaacattaaaggcctgactcaggcggcccgtaacgccaacgacggtatctccgttgcgcagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtgtgcgtgaactgacggtacaggccactaccggtactaactctgagtctgatctgtcttctatccaggacgaaattaaatcccgtctggatgaaattgaccgcgtatctggtcagacccagttcaacggcgtgaacgtgctggcaaaaaatggctccatgaaaatccaggttggcgcaaatgataaccagactatcactatcgatctgaagcagattgatgctaaaactcttggccttgatggttttagcgttaaagcgttaatcacggcttctggggatattagcttgacttttaaacaagtggatggcgtgaatgatgtaactttagagagcgtaaaagtttctagttcagcaggtacagggtactagagcacatggacatgcttatggtgtgccaccacttggataaaagcattaaagaagatgttcagttcgctgattcaaggatcggcaaactgaccgtgaccagcggccaaaaaacagaagttcaacccacgcaagtcattgatgggccttttgcgggtggcaaagacacggttgtcaatattgatcgcatcaacactaaagccgatggcacgattaaagtgggagggtttaaagcttctcttaccaccaacgcggctcatttgaatatcggcaaaggcggtgtcaatctgtccaatcaagcgagcgggcgcacccttttagtggaaaatctaaccgggaatatcaccgttgatgggcctttaagagtgaataatcaagtgggtggctatgctttggcaggatcaagcgcgaattttgaatttaaggctggtgtggatactaaaaacggcacagccactttcaataacggcaaactgaccgtgaccagcggcaaaaggaccatacagaaaaaatcagaacccgggttgttatactagatgggtgtgttggcagaagtgattaacaaaaattctaaccgaacaggggttaaagcttatgcgagcgttatcaccacgagcgatgtggcggtccaatcaggaagtttgagtaatttaactttaaatgggatccatttgggtaatatcgcagatattaagaaaaatgactcagacggaaggttagtcgcagcgatcaatgcggttacttcagaaaccggcgtggaagcttatacggatcaaaaagggcgcttgaatttgcgcagtatagatggtcgtgggattgaaatcaaaaccgatagcgtcagtaatgggcctagtgctttaacgatggtcaatggcggtcaggatttaacaaaaggttctactaactatgggaggctttctctcacacgcttagacgctaaaagcatcaatgtcgtttcggcttctgattcgcaacatttaggtttcacagcgattggttttggggaatctcaagtggcagaaaccacggtgaatttgcgcgatgttactgggaattttaacgctaatgtcaaatcagccagtggcgcgaactataacgccgtgatcgctagcggtaaccaaagcttgggatctggggttacaaccttaagagctgttgcaaatggtaaaaccacggatccgctgaaagcgctggacgatgctatcgcatctgtagacaaattccgttcttccctcggtgcggtgcaaaaccgtctggattccgcggttactaacctgaacaacaccactaccaacctgtctgaagcgcagtcccgtattcaggacgccgactatgcgaccgaagtgtccaatatgtcgaaagcgcagatcatccagcaggccggtaactccgtgttggcaaaagctaaccaggtaccgcagcaggttctgtctctgttacagggttactagagcgaggagaccaccaccaccaccaccactag BBa_K133139_sequence 1 cacatggacatgcttatggtgtgccaccacttggataaaagcattaaagaagatgttcagttcgctgattcaaggatcggcaaactgaccgtgaccagcggccaaaaaacagaagttcaacccacgcaagtcattgatgggccttttgcgggtggcaaagacacggttgtcaatattgatcgcatcaacactaaagccgatggcacgattaaagtgggagggtttaaagcttctcttaccaccaacgcggctcatttgaatatcggcaaaggcggtgtcaatctgtccaatcaagcgagcgggcgcacccttttagtggaaaatctaaccgggaatatcaccgttgatgggcctttaagagtgaataatcaagtgggtggctatgctttggcaggatcaagcgcgaattttgaatttaaggctggtgtggatactaaaaacggcacagccactttcaataacggcaaactgaccgtgaccagcggcaaaaggaccatacagaaaaaatcagaacccgggttgtta BBa_K133046_sequence 1 atgggtgtgttggcagaagtgattaacaaaaattctaaccgaacaggggttaaagcttatgcgagcgttatcaccacgagcgatgtggcggtccaatcaggaagtttgagtaatttaactttaaatgggatccatttgggtaatatcgcagatattaagaaaaatgactcagacggaaggttagtcgcagcgatcaatgcggttacttcagaaaccggcgtggaagcttatacggatcaaaaagggcgcttgaatttgcgcagtatagatggtcgtgggattgaaatcaaaaccgatagcgtcagtaatgggcctagtgctttaacgatggtcaatggcggtcaggatttaacaaaaggttctactaactatgggaggctttctctcacacgcttagacgctaaaagcatcaatgtcgtttcggcttctgattcgcaacatttaggtttcacagcgattggttttggggaatctcaagtggcagaaaccacggtgaatttgcgcgatgttactgggaattttaacgctaatgtcaaatcagccagtggcgcgaactataacgccgtgatcgctagcggtaaccaaagcttgggatctggggttacaaccttaagagctgttgcaaatggtaaaaccacggatccgctgaaagcgctggacgatgctatcgcatctgtagacaaattccgttcttccctcggtgcggtgcaaaaccgtctggattccgcggttactaacctgaacaacaccactaccaacctgtctgaagcgcagtcccgtattcaggacgccgactatgcgaccgaagtgtccaatatgtcgaaagcgcagatcatccagcaggccggtaactccgtgttggcaaaagctaaccaggtaccgcagcaggttctgtctctgttacagggt BBa_I712004_sequence 1 cgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaat BBa_K133036_sequence 1 cgaggagaccaccaccaccaccaccactag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z