BBa_K133036 1 RGD-His-St RGD-His affinity tag-Stop 2008-10-25T11:00:00Z 2015-05-08T01:09:55Z Part was constructed by primer annealing. This is His-tag with stop codon and RGD seguence. His-tag is used for detection of His-tagged proteins by anti-His antibodies. RGD is arginine-glycine-aspartic amino acid sequence, present on several extracellular matrix proteins. It is required for binding to integrins. false false _231_ 0 3350 9 It's complicated false Cloned into pSB1AK3 vector. false Jerneja Mori BBa_K133010 1 BBa_K133010 CF213 2008-10-29T12:00:00Z 2015-05-08T01:09:55Z Genomic DNA Chimeric flagellin false false _231_ 0 3351 9 It's complicated false None false Vid Kocar BBa_K133037 1 BBa_K133037 T7-CF213-UB33-CF215-RGD-Histop 2008-10-29T12:00:00Z 2015-05-08T01:09:55Z false true _231_ 0 3352 9 It's complicated false false Ana Lasic component1997018 1 BBa_K133010 component1997021 1 BBa_K133036 component1997020 1 BBa_K133046 component1997019 1 BBa_K133052 annotation1997019 1 BBa_K133052 range1997019 1 645 799 annotation1997021 1 BBa_K133036 range1997021 1 1723 1752 annotation1997020 1 BBa_K133046 range1997020 1 806 1714 annotation1997018 1 BBa_K133010 range1997018 1 1 636 BBa_K133046 1 BBa_K133046 CF215 2008-10-29T12:00:00Z 2015-05-08T01:09:55Z We used polimerase chain reaction from chimeric flagellin (BBa_K133016). This is a part that is composed of C-terminal end of chimeric flagellin (BBa_K133016). We used aminoacids from 215th aminoacid on. Chimeric flagellin is composed of C- and N-terminal part of E.coli flagellin and of variable region of H.pylori. The E.coli parts are responsible for binding and activation of cell membrane receptor TLR5 to initiate immune response, while H.pylori variable region was used to array the immune response against H.pylori. This part was used in combination with CF213 - chimeric flagellin (BBa_K133010). Between the CF213 and CF215 we have put H.pylori antigens in order to array the immune response against H.pylori. false false _231_ 0 3352 9 It's complicated false It was important to split the chimeric flagellin in the variable region to assure that the antigen will be in the right place and not to interrupt the 3d structure of the protein. false Ana Lasic BBa_K133052 1 BBa_K133052 UB33 2008-10-29T12:00:00Z 2015-05-08T01:09:55Z GeneArt Urease epitope false false _231_ 0 3352 9 Not in stock false none false Ana Lasic BBa_K133010_sequence 1 atggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcgtctgtcttctggcttgcgtattaacagcgcgaaggatgacgcagcgggtcaggcgattgctaaccgtttcacctctaacattaaaggcctgactcaggcggcccgtaacgccaacgacggtatctccgttgcgcagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtgtgcgtgaactgacggtacaggccactaccggtactaactctgagtctgatctgtcttctatccaggacgaaattaaatcccgtctggatgaaattgaccgcgtatctggtcagacccagttcaacggcgtgaacgtgctggcaaaaaatggctccatgaaaatccaggttggcgcaaatgataaccagactatcactatcgatctgaagcagattgatgctaaaactcttggccttgatggttttagcgttaaagcgttaatcacggcttctggggatattagcttgacttttaaacaagtggatggcgtgaatgatgtaactttagagagcgtaaaagtttctagttcagcaggtacaggg BBa_K133037_sequence 1 atggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcgtctgtcttctggcttgcgtattaacagcgcgaaggatgacgcagcgggtcaggcgattgctaaccgtttcacctctaacattaaaggcctgactcaggcggcccgtaacgccaacgacggtatctccgttgcgcagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtgtgcgtgaactgacggtacaggccactaccggtactaactctgagtctgatctgtcttctatccaggacgaaattaaatcccgtctggatgaaattgaccgcgtatctggtcagacccagttcaacggcgtgaacgtgctggcaaaaaatggctccatgaaaatccaggttggcgcaaatgataaccagactatcactatcgatctgaagcagattgatgctaaaactcttggccttgatggttttagcgttaaagcgttaatcacggcttctggggatattagcttgacttttaaacaagtggatggcgtgaatgatgtaactttagagagcgtaaaagtttctagttcagcaggtacagggtactagagcacatggacatgcttatggtgtgccaccacttggataaaagcattaaagaagatgttcagttcgctgattcaaggatccacatggacatgcttatggtgtgccaccacttggataaaagcattaaagaagatgttcagttcgctgattcaaggattactagatgggtgtgttggcagaagtgattaacaaaaattctaaccgaacaggggttaaagcttatgcgagcgttatcaccacgagcgatgtggcggtccaatcaggaagtttgagtaatttaactttaaatgggatccatttgggtaatatcgcagatattaagaaaaatgactcagacggaaggttagtcgcagcgatcaatgcggttacttcagaaaccggcgtggaagcttatacggatcaaaaagggcgcttgaatttgcgcagtatagatggtcgtgggattgaaatcaaaaccgatagcgtcagtaatgggcctagtgctttaacgatggtcaatggcggtcaggatttaacaaaaggttctactaactatgggaggctttctctcacacgcttagacgctaaaagcatcaatgtcgtttcggcttctgattcgcaacatttaggtttcacagcgattggttttggggaatctcaagtggcagaaaccacggtgaatttgcgcgatgttactgggaattttaacgctaatgtcaaatcagccagtggcgcgaactataacgccgtgatcgctagcggtaaccaaagcttgggatctggggttacaaccttaagagctgttgcaaatggtaaaaccacggatccgctgaaagcgctggacgatgctatcgcatctgtagacaaattccgttcttccctcggtgcggtgcaaaaccgtctggattccgcggttactaacctgaacaacaccactaccaacctgtctgaagcgcagtcccgtattcaggacgccgactatgcgaccgaagtgtccaatatgtcgaaagcgcagatcatccagcaggccggtaactccgtgttggcaaaagctaaccaggtaccgcagcaggttctgtctctgttacagggttactagagcgaggagaccaccaccaccaccaccactag BBa_K133046_sequence 1 atgggtgtgttggcagaagtgattaacaaaaattctaaccgaacaggggttaaagcttatgcgagcgttatcaccacgagcgatgtggcggtccaatcaggaagtttgagtaatttaactttaaatgggatccatttgggtaatatcgcagatattaagaaaaatgactcagacggaaggttagtcgcagcgatcaatgcggttacttcagaaaccggcgtggaagcttatacggatcaaaaagggcgcttgaatttgcgcagtatagatggtcgtgggattgaaatcaaaaccgatagcgtcagtaatgggcctagtgctttaacgatggtcaatggcggtcaggatttaacaaaaggttctactaactatgggaggctttctctcacacgcttagacgctaaaagcatcaatgtcgtttcggcttctgattcgcaacatttaggtttcacagcgattggttttggggaatctcaagtggcagaaaccacggtgaatttgcgcgatgttactgggaattttaacgctaatgtcaaatcagccagtggcgcgaactataacgccgtgatcgctagcggtaaccaaagcttgggatctggggttacaaccttaagagctgttgcaaatggtaaaaccacggatccgctgaaagcgctggacgatgctatcgcatctgtagacaaattccgttcttccctcggtgcggtgcaaaaccgtctggattccgcggttactaacctgaacaacaccactaccaacctgtctgaagcgcagtcccgtattcaggacgccgactatgcgaccgaagtgtccaatatgtcgaaagcgcagatcatccagcaggccggtaactccgtgttggcaaaagctaaccaggtaccgcagcaggttctgtctctgttacagggt BBa_K133052_sequence 1 cacatggacatgcttatggtgtgccaccacttggataaaagcattaaagaagatgttcagttcgctgattcaaggatccacatggacatgcttatggtgtgccaccacttggataaaagcattaaagaagatgttcagttcgctgattcaaggat BBa_K133036_sequence 1 cgaggagaccaccaccaccaccaccactag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z