LOCUS       BBa_K133041             3273 bp    DNA     linear       26-Nov-2024
DEFINITION  T7 promoter expression plasmid backbone
ACCESSION   BBa_K133041
VERSION     BBa_K133041.1  
FEATURES             Location/Qualifiers
     misc_feature    261..880
                     /label=pBR322
     misc_feature    1..21
                     /label=BioBrick suffix
     CDS             1097..1912
                     /label=kanamycin2 marker
     CDS             2067..2927
                     /label=beta-lactamase (ampicillin resistance marker)
     promoter        2969..2997
                     /label=amp promoter
     promoter        3173..3191
                     /label=T7 promoter
     misc_feature    3191..3218
                     /label=lacO regulatory site
     misc_feature    3258..3273
                     /label=partial BioBrick prefix (missing EcoRI)
ORIGIN
        1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct
       61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
      121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
      721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1021 acttggtctg acagctcgag tcccgtcaag tcagcgtaat gctctgccag tgttacaacc
     1081 aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca
     1141 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
     1201 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
     1261 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
     1321 caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga
     1381 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
     1441 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat
     1501 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
     1561 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg
     1621 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
     1681 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
     1741 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg
     1801 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
     1861 tggaatttaa tcgcggcctg gagcaagacg tttcccgttg aatatggctc ataacacccc
     1921 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt
     1981 gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata aatcgaactt
     2041 ttgctgagtt gaaggatcag ctcgagttac caatgcttaa tcagtgaggc acctatctca
     2101 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2161 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2221 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2281 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2341 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2401 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2461 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2521 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2581 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2641 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2701 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2761 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2821 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2881 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2941 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3001 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3061 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
     3121 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctgaat tgtaatacga
     3181 ctcactatag gggaattgtg agcggataac aattccccag ctcgaataat tttgtttaac
     3241 tttaagaagg agatatagcg gccgcttcta gag
//