LOCUS       BBa_K133051             2359 bp    DNA     linear       03-May-2024
DEFINITION  TetR-CF-mCherry-RGD-Histop
ACCESSION   BBa_K133051
VERSION     BBa_K133051.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     CDS             61..1605
                     /label=BBa_K133016
     misc_feature    1614..2321
                     /label=BBa_K133055
     misc_feature    2330..2359
                     /label=BBa_K133036
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 atggcacaag tcattaatac caacagcctc tcgctgatca ctcaaaataa tatcaacaag
      121 aaccagtctg cgctgtcgag ttctatcgag cgtctgtctt ctggcttgcg tattaacagc
      181 gcgaaggatg acgcagcggg tcaggcgatt gctaaccgtt tcacctctaa cattaaaggc
      241 ctgactcagg cggcccgtaa cgccaacgac ggtatctccg ttgcgcagac caccgaaggc
      301 gcgctgtccg aaatcaacaa caacttacag cgtgtgcgtg aactgacggt acaggccact
      361 accggtacta actctgagtc tgatctgtct tctatccagg acgaaattaa atcccgtctg
      421 gatgaaattg accgcgtatc tggtcagacc cagttcaacg gcgtgaacgt gctggcaaaa
      481 aatggctcca tgaaaatcca ggttggcgca aatgataacc agactatcac tatcgatctg
      541 aagcagattg atgctaaaac tcttggcctt gatggtttta gcgttaaagc gttaatcacg
      601 gcttctgggg atattagctt gacttttaaa caagtggatg gcgtgaatga tgtaacttta
      661 gagagcgtaa aagtttctag ttcagcaggc acggggatcg gtgtgttagc ggaagtgatt
      721 aacaaaaatt ctaaccgaac aggggttaaa gcttatgcga gcgttatcac cacgagcgat
      781 gtggcggtcc aatcaggaag tttgagtaat ttaactttaa atgggatcca tttgggtaat
      841 atcgcagata ttaagaaaaa tgactcagac ggaaggttag tcgcagcgat caatgcggtt
      901 acttcagaaa ccggcgtgga agcttatacg gatcaaaaag ggcgcttgaa tttgcgcagt
      961 atagatggtc gtgggattga aatcaaaacc gatagcgtca gtaatgggcc tagtgcttta
     1021 acgatggtca atggcggtca ggatttaaca aaaggttcta ctaactatgg gaggctttct
     1081 ctcacacgct tagacgctaa aagcatcaat gtcgtttcgg cttctgattc gcaacattta
     1141 ggtttcacag cgattggttt tggggaatct caagtggcag aaaccacggt gaatttgcgc
     1201 gatgttactg ggaattttaa cgctaatgtc aaatcagcca gtggcgcgaa ctataacgcc
     1261 gtgatcgcta gcggtaacca aagcttggga tctggggtta caaccttaag agctgttgca
     1321 aatggtaaaa ccacggatcc gctgaaagcg ctggacgatg ctatcgcatc tgtagacaaa
     1381 ttccgttctt ccctcggtgc ggtgcaaaac cgtctggatt ccgcggttac caacctgaac
     1441 aacaccacta ccaacctgtc tgaagcgcag tcccgtattc aggacgccga ctatgcgacc
     1501 gaagtgtcca atatgtcgaa agcgcagatc atccagcagg ccggtaactc cgtgttggca
     1561 aaagctaacc aggtaccgca gcaggttctg tctctgttac agggttacta gagataacat
     1621 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     1681 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     1741 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     1801 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     1861 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     1921 gaccgtgacc caggactcct ccctccagga cggcgagttc atctacaagg tgaagctgcg
     1981 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     2041 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     2101 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     2161 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     2221 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     2281 catggacgag ctgtacaagg atccaaaaaa gaagagaaag gtactagagc gaggagacca
     2341 ccaccaccac caccactag
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