BBa_K133133
1
His-Stop
His affinity tag-stop
2008-10-22T11:00:00Z
2015-05-08T01:09:57Z
Part was constructed by primer annealing.
This is His-tag with stop codon. His-tag is used for detection of His-tagged proteins by anti-His antibodies. Cloned into pSB1AK3 vector.
false
false
_231_
0
1883
9
It's complicated
false
false
Katja Kolar
BBa_K133087
1
BBa_K133087
CMV-SS-gyrHP-linker-(TMTIR)TLR9-GFP-Histop
2008-10-29T12:00:00Z
2015-05-08T01:09:56Z
false
false
_231_
0
3358
9
It's complicated
false
false
Jan Lonzaric
component1995682
1
BBa_I712009
component1995686
1
BBa_K133066
component1995685
1
BBa_K133060
component1995684
1
BBa_K133132
component1995681
1
BBa_I712004
component1995687
1
BBa_K133133
component1995683
1
BBa_K133062
annotation1995683
1
BBa_K133062
range1995683
1
742
1401
annotation1995687
1
BBa_K133133
range1995687
1
2818
2838
annotation1995682
1
BBa_I712009
range1995682
1
661
735
annotation1995685
1
BBa_K133060
range1995685
1
1442
2086
annotation1995681
1
BBa_I712004
range1995681
1
1
654
annotation1995684
1
BBa_K133132
range1995684
1
1410
1433
annotation1995686
1
BBa_K133066
range1995686
1
2093
2809
BBa_K133062
1
BBa_K133062
gyrHP
2008-10-23T11:00:00Z
2015-05-08T01:09:56Z
false
true
_231_
0
3358
9
It's complicated
false
false
Jan Lonzaric
BBa_K133060
1
BBa_K133060
(TMTIR)TLR9
2008-10-21T11:00:00Z
2015-05-08T01:09:56Z
amplified from TLR9 gene, obtained from Invivogen, plasmid pUNO-hTLR9
Toll-Like Receptor 9 transmembrane region and cytoplasmic domain.
false
false
_231_
0
1883
9
It's complicated
false
false
Katja Kolar
BBa_K133132
1
linker
8 aa protein domain linker
2008-10-23T11:00:00Z
2015-05-08T01:09:57Z
false
false
_231_
0
3358
9
It's complicated
false
false
Jan Lonzaric
BBa_K133066
1
BBa_K133066
GFP
2008-10-21T11:00:00Z
2015-05-08T01:09:56Z
Green fluorescence protein (mCerulean) WITHOUT stop codon.
false
false
_231_
0
1883
9
It's complicated
false
false
Katja Kolar
BBa_I712004
1
BBa_I712004
CMV promoter
2007-10-18T11:00:00Z
2015-08-31T04:07:45Z
pcDNA3 by Invitrogen
a constitutive expression promoter or use in mammalian cells. Ribosome binding site is included.
false
false
_130_
0
1896
9
It's complicated
false
Cloned into pSB1AC3 vector.
false
Andrej Ondracka
BBa_I712009
1
CD4 signal
CD4 signal peptide; localizes to plasma membrane
2007-10-21T11:00:00Z
2015-08-31T04:07:45Z
It comes from human gene for CD4 receptor.
Coding region for first 25 amino acids (including start codon) of CD4 receptor which is signal peptide for plasma membrane localization.
false
false
_130_
0
1850
9
In stock
false
No considerations.
true
Peter Cimermancic
BBa_I712009_sequence
1
atgaaccggggagtcccttttaggcacttgcttctggtgctgcaactggcgctcctcccagcagccactcaggga
BBa_K133087_sequence
1
cgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaattactagatgaaccggggagtcccttttaggcacttgcttctggtgctgcaactggcgctcctcccagcagccactcagggatactagatgcaaaattaccagagccatagtattaaggttttgaaaggtttagagggggttaggaaacgccctggaatgtatattggcgataccaatgtgggtgggttgcaccacatggtgtatgaagtcgtggataacgctgtagatgagagcatggcgggtttttgcgatacgattaatatcactttgactgatgagggttcatgcattgtagaagataacgggcgaggcattcctgtagatattcaccccacggaaaaaatccccgcttgcaccgtggttctaacgattttgcgtgcgggaggcaagtttgataatgatacttataaagtttcaggcggtttgcatggcgtgggcgtttcggttgtgaacgctttgagcaaacgcttgatcatgacgattaaaaaagagggtcaaatctatcgccaagagtttgaaaagggtattcctactagcgagcttgaaatcattggcaaaaccaaaagtgctaaagaaagcggcacgactattgaatttttccctgatgaaagcgtgatggaagtcgttgaatttcaagcgggtattttgcaaaaacgctttaaagaaatggcgtatcttaacgacggcttaaaaatttctttcaaagaagaaaaaacccaactacaagaaacttatttctataaagactactagagtccgcttgttactgtgagctttcctactagagtgtttcgccctctcgctgctggctgtggctctgggcctgggtgtgcccatgctgcatcacctctgtggctgggacctctggtactgcttccacctgtgcctggcctggcttccctggcgggggcggcaaagtgggcgagatgaggatgccctgccctacgatgccttcgtggtcttcgacaaaacgcagagcgcagtggcagactgggtgtacaacgagcttcgggggcagctggaggagtgccgtgggcgctgggcactccgcctgtgcctggaggaacgcgactggctgcctggcaaaaccctctttgagaacctgtgggcctcggtctatggcagccgcaagacgctgtttgtgctggcccacacggaccgggtcagtggtctcttgcgcgccagcttcctgctggcccagcagcgcctgctggaggaccgcaaggacgtcgtggtgctggtgatcctgagccctgacggccgccgctcccgctacgtgcggctgcgccagcgcctctgccgccagagtgtcctcctctggccccaccagcccagtggtcagcgcagcttctgggcccagctgggcatggccctgaccagggacaaccaccacttctataaccggaacttctgccagggacccaccgctgaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaacgccatcagcgacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccaagctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtactagagcaccaccaccaccaccactag
BBa_K133060_sequence
1
tgtttcgccctctcgctgctggctgtggctctgggcctgggtgtgcccatgctgcatcacctctgtggctgggacctctggtactgcttccacctgtgcctggcctggcttccctggcgggggcggcaaagtgggcgagatgaggatgccctgccctacgatgccttcgtggtcttcgacaaaacgcagagcgcagtggcagactgggtgtacaacgagcttcgggggcagctggaggagtgccgtgggcgctgggcactccgcctgtgcctggaggaacgcgactggctgcctggcaaaaccctctttgagaacctgtgggcctcggtctatggcagccgcaagacgctgtttgtgctggcccacacggaccgggtcagtggtctcttgcgcgccagcttcctgctggcccagcagcgcctgctggaggaccgcaaggacgtcgtggtgctggtgatcctgagccctgacggccgccgctcccgctacgtgcggctgcgccagcgcctctgccgccagagtgtcctcctctggccccaccagcccagtggtcagcgcagcttctgggcccagctgggcatggccctgaccagggacaaccaccacttctataaccggaacttctgccagggacccaccgctgaa
BBa_I712004_sequence
1
cgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaat
BBa_K133133_sequence
1
caccaccaccaccaccactag
BBa_K133066_sequence
1
atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaacgccatcagcgacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccaagctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaag
BBa_K133132_sequence
1
tccgcttgttactgtgagctttcc
BBa_K133062_sequence
1
atgcaaaattaccagagccatagtattaaggttttgaaaggtttagagggggttaggaaacgccctggaatgtatattggcgataccaatgtgggtgggttgcaccacatggtgtatgaagtcgtggataacgctgtagatgagagcatggcgggtttttgcgatacgattaatatcactttgactgatgagggttcatgcattgtagaagataacgggcgaggcattcctgtagatattcaccccacggaaaaaatccccgcttgcaccgtggttctaacgattttgcgtgcgggaggcaagtttgataatgatacttataaagtttcaggcggtttgcatggcgtgggcgtttcggttgtgaacgctttgagcaaacgcttgatcatgacgattaaaaaagagggtcaaatctatcgccaagagtttgaaaagggtattcctactagcgagcttgaaatcattggcaaaaccaaaagtgctaaagaaagcggcacgactattgaatttttccctgatgaaagcgtgatggaagtcgttgaatttcaagcgggtattttgcaaaaacgctttaaagaaatggcgtatcttaacgacggcttaaaaatttctttcaaagaagaaaaaacccaactacaagaaacttatttctataaagac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z