BBa_K133133
1
His-Stop
His affinity tag-stop
2008-10-22T11:00:00Z
2015-05-08T01:09:57Z
Part was constructed by primer annealing.
This is His-tag with stop codon. His-tag is used for detection of His-tagged proteins by anti-His antibodies. Cloned into pSB1AK3 vector.
false
false
_231_
0
1883
9
It's complicated
false
false
Katja Kolar
BBa_K133135
1
BBa_K133135
CMV-SS-HA-UreB-CD4sh-(TIR)TLR3-Histop
2008-10-29T12:00:00Z
2015-05-08T01:09:57Z
false
false
_231_
0
3358
9
It's complicated
false
false
Jan Lonzaric
component1996972
1
BBa_I712009
component1996976
1
BBa_K133133
component1996971
1
BBa_I712004
component1996974
1
BBa_K133071
component1996975
1
BBa_K133068
component1996973
1
BBa_I712008
annotation1996972
1
BBa_I712009
range1996972
1
661
735
annotation1996975
1
BBa_K133068
range1996975
1
2493
4139
annotation1996976
1
BBa_K133133
range1996976
1
4148
4168
annotation1996974
1
BBa_K133071
range1996974
1
778
2484
annotation1996971
1
BBa_I712004
range1996971
1
1
654
annotation1996973
1
BBa_I712008
range1996973
1
742
771
BBa_I712004
1
BBa_I712004
CMV promoter
2007-10-18T11:00:00Z
2015-08-31T04:07:45Z
pcDNA3 by Invitrogen
a constitutive expression promoter or use in mammalian cells. Ribosome binding site is included.
false
false
_130_
0
1896
9
It's complicated
false
Cloned into pSB1AC3 vector.
false
Andrej Ondracka
BBa_I712009
1
CD4 signal
CD4 signal peptide; localizes to plasma membrane
2007-10-21T11:00:00Z
2015-08-31T04:07:45Z
It comes from human gene for CD4 receptor.
Coding region for first 25 amino acids (including start codon) of CD4 receptor which is signal peptide for plasma membrane localization.
false
false
_130_
0
1850
9
In stock
false
No considerations.
true
Peter Cimermancic
BBa_K133071
1
BBa_K133071
UreB
2008-10-21T11:00:00Z
2015-05-08T01:09:56Z
Cloned into pSB1AK3 vector.
false
false
_231_
0
1883
9
It's complicated
false
false
Katja Kolar
BBa_K133068
1
BBa_K133068
CD4sh-(TIR)TLR3
2008-10-29T12:00:00Z
2015-05-08T01:09:56Z
false
false
_231_
0
1883
9
It's complicated
false
false
Katja Kolar
BBa_I712008
1
HA
HA affinity tag
2007-10-19T11:00:00Z
2015-08-31T04:07:45Z
Part was constructed by primer annealing.
HA-tag is used for detection of HA-tagged proteins by anti-HA antibodies.
false
false
_130_
0
1846
9
It's complicated
false
Gene was designed to be constructed by primer annealing. Includes start codon.
false
Anja Korenčič
BBa_I712009_sequence
1
atgaaccggggagtcccttttaggcacttgcttctggtgctgcaactggcgctcctcccagcagccactcaggga
BBa_K133071_sequence
1
atgaaaaagattagcagaaaagaatatgtttctatgtatggccctactacaggcgataaagtgagattgggcgatacagacttgatcgctgaagtagaacatgactacaccatttatggcgaagagcttaaattcggtggcggtaaaaccctaagagaaggcatgagccaatctaacaatcctagcaaagaagaactggatttaatcatcactaacgctttaatcgtggattacaccggtatttataaagcggatattggtattaaagatggcaaaatcgctggcattggtaaaggcggtaacaaagacatgcaagatggcgttaaaaacaatcttagcgtgggtcctgctactgaagccttagccggtgaaggtttgatcgtaactgctggtggtattgacacacacatccacttcatctccccccaacaaatccctacagcttttgcaagcggtgtaacaaccatgattggtggcggaactggccctgctgatggcactaacgcaaccactatcactccaggcagaagaaatttaaaattcatgctcagagcggctgaagaatattctatgaactttggtttcttggctaaaggtaacgtttctaacgatgcgagcttagccgatcaaattgaagctggtgcgattggctttaaaatccacgaagactggggtaccactccttctgcaatcaatcatgcgttagatgttgcagacaaatacgatgtgcaagtcgctatccacacagacactttgaatgaagccggttgcgtggaagacactatggcagccattgccggacgcactatgcacactttccacactgaaggcgctggcggcggacacgctcctgatattattaaagtggccggtgaacacaacattctacctgcttccactaaccccactatccctttcaccgtgaatacagaagccgaacacatggacatgcttatggtgtgccaccacttggataaaagcattaaagaagatgttcagttcgctgattcaaggatccgccctcaaaccattgcggctgaagacactttgcatgacatggggattttctcaatcaccagttctgactctcaagctatgggtcgtgtgggtgaagttatcaccagaacttggcaaacagctgacaaaaacaaaaaagaatttggccgcttgaaagaagaaaaaggcgataacgacaacttcaggatcaaacgctacttgtctaaatacaccattaacccagcgatcgctcatgggattagcgagtatgtcggttctgtagaagtgggcaaagtggctgacttggtattgtggagtccagcattctttggtgtgaaacccaacatgatcatcaaaggcggattcattgcattgagtcaaatgggtgatgcgaacgcttctatccctaccccacaaccggtttattacagagaaatgttcgctcatcatggtaaagctaaatacgatgcaaacatcacttttgtgtctcaagcggcttatgacaaaggcattaaagaagaattagggcttgaaagacaagtgttgccggtaaaaaattgcagaaacatcactaaaaaagacatgcaattcaacgacactaccgctcacattgaagtcaatcctgaaacttaccatgtgttcgtggatggcaaagaagtaacttctaaaccagccaataaagtgagcttggcgcaactctttagcattttc
BBa_I712004_sequence
1
cgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaat
BBa_K133133_sequence
1
caccaccaccaccaccactag
BBa_I712008_sequence
1
atgtacccctacgacgtgcccgactacgcc
BBa_K133135_sequence
1
cgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaattactagatgaaccggggagtcccttttaggcacttgcttctggtgctgcaactggcgctcctcccagcagccactcagggatactagatgtacccctacgacgtgcccgactacgcctactagatgaaaaagattagcagaaaagaatatgtttctatgtatggccctactacaggcgataaagtgagattgggcgatacagacttgatcgctgaagtagaacatgactacaccatttatggcgaagagcttaaattcggtggcggtaaaaccctaagagaaggcatgagccaatctaacaatcctagcaaagaagaactggatttaatcatcactaacgctttaatcgtggattacaccggtatttataaagcggatattggtattaaagatggcaaaatcgctggcattggtaaaggcggtaacaaagacatgcaagatggcgttaaaaacaatcttagcgtgggtcctgctactgaagccttagccggtgaaggtttgatcgtaactgctggtggtattgacacacacatccacttcatctccccccaacaaatccctacagcttttgcaagcggtgtaacaaccatgattggtggcggaactggccctgctgatggcactaacgcaaccactatcactccaggcagaagaaatttaaaattcatgctcagagcggctgaagaatattctatgaactttggtttcttggctaaaggtaacgtttctaacgatgcgagcttagccgatcaaattgaagctggtgcgattggctttaaaatccacgaagactggggtaccactccttctgcaatcaatcatgcgttagatgttgcagacaaatacgatgtgcaagtcgctatccacacagacactttgaatgaagccggttgcgtggaagacactatggcagccattgccggacgcactatgcacactttccacactgaaggcgctggcggcggacacgctcctgatattattaaagtggccggtgaacacaacattctacctgcttccactaaccccactatccctttcaccgtgaatacagaagccgaacacatggacatgcttatggtgtgccaccacttggataaaagcattaaagaagatgttcagttcgctgattcaaggatccgccctcaaaccattgcggctgaagacactttgcatgacatggggattttctcaatcaccagttctgactctcaagctatgggtcgtgtgggtgaagttatcaccagaacttggcaaacagctgacaaaaacaaaaaagaatttggccgcttgaaagaagaaaaaggcgataacgacaacttcaggatcaaacgctacttgtctaaatacaccattaacccagcgatcgctcatgggattagcgagtatgtcggttctgtagaagtgggcaaagtggctgacttggtattgtggagtccagcattctttggtgtgaaacccaacatgatcatcaaaggcggattcattgcattgagtcaaatgggtgatgcgaacgcttctatccctaccccacaaccggtttattacagagaaatgttcgctcatcatggtaaagctaaatacgatgcaaacatcacttttgtgtctcaagcggcttatgacaaaggcattaaagaagaattagggcttgaaagacaagtgttgccggtaaaaaattgcagaaacatcactaaaaaagacatgcaattcaacgacactaccgctcacattgaagtcaatcctgaaacttaccatgtgttcgtggatggcaaagaagtaacttctaaaccagccaataaagtgagcttggcgcaactctttagcattttctactagagaagaaagtggtgctgggcaaaaaaggggatacagtggaactgacctgtacagcttcccagaagaagagcatacaattccactggaaaaactccaaccagataaagattctgggaaatcagggcttcttcttaactaaaggtccatccaagctgaatgatcgcgctgactcaagaagaagcctttgggaccaaggaaactttcccctgatcatcaagaatcttaagatagaagactcagatacttacatctgtgaagtggaggaccagaaggaggaggtgcaattgctagtgttcggattgactgccaactctgacacccacctgcttcaggggcagagcctgaccctgaccttggagagcccccctggtagtagcccctcagtgcaatgtaggagtccaaggggtaaaaacatacagggggggaagaccctctccgtgtctcagctggagctccaggatagtggcacctggacatgcactgtcttgcagaaccagaagaaggtggagttcaaaatagacatcgtggtgctagctttccagaaggcctccagcatagtctataagaaagagggggaacaggtggagttctccttcccactcgcctttacagttgaaaagctgacgggcagtggcgagctgtggtggcaggcggagagggcttcctcctccaagtcttggatcacctttgacctgaagaacaaggaagtgtctgtaaaacgggttacccaggaccctaagctccagatgggcaagaagctcccgctccacctcaccctgccccaggccttgcctcagtatgctggctctggaaacctcaccctggcccttgaagcgaaaacaggaaagttgcatcaggaagtgaacctggtggtgatgagagccactcagctccagaaaaatttgacctgtgaggtgtggggacccacctcccctaagctgatgctgagtttgaaactggagaacaaggaggcaaaggtctcgaagcgggagaaggcggtgtgggtgctgaaccctgaggcggggatgtggcagtgtctgctgagtgactcgggacaggtcctgctggaatccaacatcaaggttctgcccacatggtccaccccggtgcagccaatggccctgattgtgctggggggcgtcgccggcctcctgcttttcattgggctaggcatcttcttctgtgtcaggactagatttgaatatgcagcatatataattcatgcctataaagataaggattgggtctgggaacatttctcttcaatggaaaaggaagaccaatctctcaaattttgtctggaagaaagggactttgaggcgggtgtttttgaactagaagcaattgttaacagcatcaaaagaagcagaaaaattatttttgttataacacaccatctattaaaagacccattatgcaaaagattcaaggtacatcatgcagttcaacaagctattgaacaaaatctggattccattatattggttttccttgaggagattccagattataaactgaaccatgcactctgtttgcgaagaggaatgtttaaatctcactgcatcttgaactggccagttcagaaagaacggataggtgcctttcgtcataaattgcaagtagcacttggatccaaaaactctgtacattactagagcaccaccaccaccaccactag
BBa_K133068_sequence
1
aagaaagtggtgctgggcaaaaaaggggatacagtggaactgacctgtacagcttcccagaagaagagcatacaattccactggaaaaactccaaccagataaagattctgggaaatcagggcttcttcttaactaaaggtccatccaagctgaatgatcgcgctgactcaagaagaagcctttgggaccaaggaaactttcccctgatcatcaagaatcttaagatagaagactcagatacttacatctgtgaagtggaggaccagaaggaggaggtgcaattgctagtgttcggattgactgccaactctgacacccacctgcttcaggggcagagcctgaccctgaccttggagagcccccctggtagtagcccctcagtgcaatgtaggagtccaaggggtaaaaacatacagggggggaagaccctctccgtgtctcagctggagctccaggatagtggcacctggacatgcactgtcttgcagaaccagaagaaggtggagttcaaaatagacatcgtggtgctagctttccagaaggcctccagcatagtctataagaaagagggggaacaggtggagttctccttcccactcgcctttacagttgaaaagctgacgggcagtggcgagctgtggtggcaggcggagagggcttcctcctccaagtcttggatcacctttgacctgaagaacaaggaagtgtctgtaaaacgggttacccaggaccctaagctccagatgggcaagaagctcccgctccacctcaccctgccccaggccttgcctcagtatgctggctctggaaacctcaccctggcccttgaagcgaaaacaggaaagttgcatcaggaagtgaacctggtggtgatgagagccactcagctccagaaaaatttgacctgtgaggtgtggggacccacctcccctaagctgatgctgagtttgaaactggagaacaaggaggcaaaggtctcgaagcgggagaaggcggtgtgggtgctgaaccctgaggcggggatgtggcagtgtctgctgagtgactcgggacaggtcctgctggaatccaacatcaaggttctgcccacatggtccaccccggtgcagccaatggccctgattgtgctggggggcgtcgccggcctcctgcttttcattgggctaggcatcttcttctgtgtcaggactagatttgaatatgcagcatatataattcatgcctataaagataaggattgggtctgggaacatttctcttcaatggaaaaggaagaccaatctctcaaattttgtctggaagaaagggactttgaggcgggtgtttttgaactagaagcaattgttaacagcatcaaaagaagcagaaaaattatttttgttataacacaccatctattaaaagacccattatgcaaaagattcaaggtacatcatgcagttcaacaagctattgaacaaaatctggattccattatattggttttccttgaggagattccagattataaactgaaccatgcactctgtttgcgaagaggaatgtttaaatctcactgcatcttgaactggccagttcagaaagaacggataggtgcctttcgtcataaattgcaagtagcacttggatccaaaaactctgtacat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z