BBa_K1333000 1 BBa_K1333000 M13 bacteriophage g1p with a FLAG tag on C-terminal 2014-09-22T11:00:00Z 2015-05-08T01:09:57Z It is derived from the commercial M13KE vector(New England Biolab) used in phage display system by PCR. This part is coding the M13 bacteriophage g1p with a FLAG tag on C-terminal, which is an assembly protein of M13 bacteriophage spanning on the inner membrane of the infected Escherichia coli. Its function is to initiate the phage assembly by interacting with the viral g4p protein and host-encoded thioredoxin and recognizing the packaging signal in phage's single strand DNA. false false _1708_ 0 18998 9 It's complicated true Between the g1p and FLAG coding sequencing, there are 9 nucleotides linker sequence coding an RRS linker peptide. It is not certain whether this peptide would affect the regular function of g1p protein. false Huang Junxiang, Wang Xizi annotation2385431 1 linker range2385431 1 1045 1053 annotation2385430 1 FLAG range2385430 1 1054 1080 annotation2385429 1 g1p range2385429 1 1 1044 BBa_K1333000_sequence 1 atggctgtttattttgtaactggcaaattaggctctggaaagacgctcgttagcgttggtaagattcaggataaaattgtagctgggtgcaaaatagcaactaatcttgatttaaggcttcaaaacctcccgcaagtcgggaggttcgctaaaacgcctcgcgttcttagaataccggataagccttctatatctgatttgcttgctattgggcgcggtaatgattcctacgatgaaaataaaaacggcttgcttgttctcgatgagtgcggtacttggtttaatacccgttcttggaatgataaggaaagacagccgattattgattggtttctacatgctcgtaaattaggatgggatattatttttcttgttcaggacttatctattgttgataaacaggcgcgttctgcattagctgaacatgttgtttattgtcgtcgtctggacagaattactttaccttttgtcggtactttatattctcttattactggctcgaaaatgcctctgcctaaattacatgttggcgttgttaaatatggcgattctcaattaagccctactgttgagcgttggctttatactggtaagaatttgtataacgcatatgatactaaacaggctttttctagtaattatgattccggtgtttattcttatttaacgccttatttatcacacggtcggtatttcaaaccattaaatttaggtcagaagatgaaattaactaaaatatatttgaaaaagttttctcgcgttctttgtcttgcgattggatttgcatcagcatttacatatagttatataacccaacctaagccggaggttaaaaaggtagtctctcagacctatgattttgataaattcactattgactcttctcagcgtcttaatctaagctatcgctatgttttcaaggattctaagggaaaattaattaatagcgacgatttacagaagcaaggttattcactcacatatattgatttatgtactgtttccattaaaaaaggtaattcaaatgaaattgttaaatgtaatagatcatcagactacaaagatgacgacgataaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z