BBa_K1333200 1 BBa_K1333200 lambda cI-LGF2(E75A) 2014-09-22T11:00:00Z 2015-05-08T01:09:57Z It is from Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit and modified by Site-Directed Mutagenesis(SDM). Fuse lambda cI with LGF2 mutated from Glu to Ala at site 75 (the Glu which locates after three continuous Ala behind lambda repressor is at site 68). As one of the components in Bacterial Two-hybrid System, it causes little interaction with GAL11P compared to the normalized WT. false false _1708_ 0 15838 9 In stock false The LGF2 is mutated from GAA to GCA at site 75. false Yanwen Xie annotation2385727 1 TGA stop codon range2385727 1 841 843 annotation2385724 1 lambda cI range2385724 1 1 711 annotation2385725 1 ATG start codon range2385725 1 1 3 annotation2385726 1 LGF2(E75A) range2385726 1 721 840 BBa_K1333200_sequence 1 atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgcggccgcagaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagcagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattattttga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z