BBa_K1333202 1 BBa_K1333202 lambda cI-LGF2(R74A) 2014-09-22T11:00:00Z 2015-05-08T01:09:58Z It is from Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit and modified by Site-Directed Mutagenesis(SDM). Fuse lambda cI with LGF2 mutated from Arg to Ala at site 74 (the Glu which locates after three continuous Ala behind lambda repressor is at site 58). As one of the components in Bacterial Two-hybrid System, it causes a stronger interaction with GAL11P compared to the normalized WT. false false _1708_ 0 15838 9 Not in stock false The LGF2 is mutated from CGA to GCA at site 74. false Yanwen Xie annotation2385731 1 LGF2(R74A) range2385731 1 721 840 annotation2385729 1 TGA stop codon range2385729 1 841 843 annotation2385728 1 ATG start codon range2385728 1 1 3 annotation2385730 1 lambda cI range2385730 1 1 711 BBa_K1333202_sequence 1 atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgcggccgcagaatcaaggctagaaagactggaacagctatttctactgatttttcctgcggaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattattttga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z