BBa_I13453
1
BBa_I13453
Pbad promoter
2005-05-24T11:00:00Z
2015-08-31T04:07:34Z
Released HQ 2013
PBad promoter from I0500 without AraC.
false
false
_11_
0
253
6
In stock
false
true
jkm
BBa_K1333304
1
BBa_K1333304
I13453-PrfA
2014-09-23T11:00:00Z
2015-05-08T01:09:58Z
The source of the part will be its components.
This part is a combination of a pBAD promoter(BBa_I13453), RNA thermometer(BBa_K115003)
false
false
_1708_
0
15873
9
In stock
false
false
Runwen Yao
component2385932
1
BBa_K115003
component2385929
1
BBa_I13453
annotation2385929
1
BBa_I13453
range2385929
1
1
130
annotation2385932
1
BBa_K115003
range2385932
1
139
247
BBa_K115003
1
BBa_K115003
RNA thermometer (PrfA)
2008-07-14T11:00:00Z
2015-05-08T01:09:27Z
This sequence is taken from the Listeria Monocytogenes (EU372032.). It's the 5'UTR of a TF induced at 37 degrees.
Released HQ 2013
This part is designed as a temperature inducible RBS. Only designed so far.
false
false
_223_
0
3007
9
In stock
true
The secondary structure is important to the function of these regions, but part of the wt secondary structure is destroyed by the scar. We've tried to alter the sequence so the predicted structure (through mfold and those kind of servers) is sort of conserved, but temperature sensitivity still has to be tested. If it doesn't work, possible solution might be the addition of a larger conserved part of the wt, which implies a small part of wt protein sequence as well.
true
O.M.J.A. Stassen
annotation1966983
1
SD
range1966983
1
106
109
annotation1967003
1
Predicted stem loop up to A of AUG
range1967003
1
28
109
BBa_I13453_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
BBa_K1333304_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagtgtaaaaaatattatttagcgtgactttctagtaacagctaacaattgttgttactgcctaatgtttttagggtattttaaaaaagggcgataaaaaacgattggggga
BBa_K115003_sequence
1
tgtaaaaaatattatttagcgtgactttctagtaacagctaacaattgttgttactgcctaatgtttttagggtattttaaaaaagggcgataaaaaacgattggggga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z