LOCUS       BBa_K1333310             901 bp    DNA     linear       23-Dec-2024
DEFINITION  RFP with an RNA thermometer(FourU)
ACCESSION   BBa_K1333310
VERSION     BBa_K1333310.1  
FEATURES             Location/Qualifiers
     RBS             47..52
                     /label=rbs
     misc_feature    23..28
                     /label=scar adaptation
     stem_loop       1..18
                     /label=stem_loop
     stem_loop       24..52
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    1..52
                     /label=BBa_K115002
     CDS             59..733
                     /label=mrfp1
     misc_feature    740..764
                     /label=Help:Barcodes
     CDS             59..764
                     /label=BBa_E1010
     stem_loop       784..827
                     /label=stem_loop
     misc_feature    773..852
                     /label=BBa_B0010
     terminator      773..852
                     /label=BBa_B0010
     stem_loop       868..887
                     /label=T7 TE
     misc_feature    894..894
                     /label=stop
     polyA_site      888..901
                     /label=polya
     misc_feature    861..901
                     /label=BBa_B0012
     terminator      861..901
                     /label=BBa_B0012
     terminator      773..901
                     /label=BBa_B0015
ORIGIN
        1 ggacaagcaa tgcttgcctt gaatagtaac ttttgaatag tgattcagga ggtactagat
       61 ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc
      121 cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac
      181 ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct
      241 gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga
      301 ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga
      361 cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa
      421 agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat
      481 gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat
      541 caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta
      601 catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat
      661 cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca
      721 ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat
      781 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      841 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      901 a
//