##gff-version 3
##sequence-region BBa_K1333310 1 901
BBa_K1333310	.	mature_transcript_region	1	52	.	+	0	ID=BBa_K115002;Name=BBa_K115002
BBa_K1333310	.	stem_loop	1	18	.	+	0	ID=annotation1966930;Name=stem_loop;Parent=BBa_K115002
BBa_K1333310	.	ribosome_entry_site	47	52	.	+	0	ID=annotation1966897;Name=rbs;Parent=BBa_K115002
BBa_K1333310	.	sequence_alteration	23	28	.	+	0	ID=annotation1966899;Name=scar adaptation;Parent=BBa_K115002
BBa_K1333310	.	stem_loop	24	52	.	+	0	ID=annotation1966931;Name=predicted stem-loop extending to one position before start codon;Parent=BBa_K115002
BBa_K1333310	.	terminator	773	901	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1333310	.	terminator	773	852	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1333310	.	engineered_region	773	852	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1333310	.	stem_loop	784	827	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1333310	.	terminator	861	901	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1333310	.	engineered_region	861	901	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1333310	.	stop_codon	894	894	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1333310	.	stem_loop	868	887	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1333310	.	polyA_site	888	901	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1333310	.	CDS	59	764	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K1333310	.	engineered_tag	740	764	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1333310	.	CDS	59	733	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
>BBa_K1333310
ggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggaggtactagatggcttcctccgaagacgtt
atcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtg
aaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacat
cctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcc
ttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccc
tgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaa
aaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctg
aaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtg
cttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctga
aggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttata