LOCUS       BBa_K1333311             958 bp    DNA     linear       26-Nov-2024
DEFINITION  RFP with an RNA thermometer(PrfA)
ACCESSION   BBa_K1333311
VERSION     BBa_K1333311.1  
FEATURES             Location/Qualifiers
     RBS             106..109
                     /label=SD
     stem_loop       28..109
                     /label=Predicted stem loop up to A of AUG
     misc_feature    1..109
                     /label=BBa_K115003
     CDS             116..790
                     /label=mrfp1
     misc_feature    797..821
                     /label=Help:Barcodes
     CDS             116..821
                     /label=BBa_E1010
     stem_loop       841..884
                     /label=stem_loop
     misc_feature    830..909
                     /label=BBa_B0010
     terminator      830..909
                     /label=BBa_B0010
     stem_loop       925..944
                     /label=T7 TE
     misc_feature    951..951
                     /label=stop
     polyA_site      945..958
                     /label=polya
     misc_feature    918..958
                     /label=BBa_B0012
     terminator      918..958
                     /label=BBa_B0012
     terminator      830..958
                     /label=BBa_B0015
ORIGIN
        1 tgtaaaaaat attatttagc gtgactttct agtaacagct aacaattgtt gttactgcct
       61 aatgttttta gggtatttta aaaaagggcg ataaaaaacg attgggggat actagatggc
      121 ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt
      181 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca
      241 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc
      301 cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta
      361 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg
      421 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt
      481 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg
      541 ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa
      601 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat
      661 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac
      721 ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc
      781 caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa
      841 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      901 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//