LOCUS       BBa_K1333312            2619 bp    DNA     linear       22-Nov-2024
DEFINITION  I13458-I13453-RBS-G3P(M13)
ACCESSION   BBa_K1333312
VERSION     BBa_K1333312.1  
FEATURES             Location/Qualifiers
     misc_feature    1..1114
                     /label=BBa_I13458
     promoter        1123..1252
                     /label=BBa_I13453
     misc_feature    1265..1268
                     /label=conserved
     RBS             1261..1272
                     /label=BBa_B0034
     CDS             1279..2586
                     /label=g3p with an 12 AAs peptide insertion designed for 
                     phage display
     CDS             2593..2619
                     /label=FLAG
     CDS             1329..1365
                     /label=12 AAs peptide insertion designed for phage display
     CDS             1279..2619
                     /label=BBa_K1333002
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctattactag agacattgat tatttgcacg
     1141 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     1201 gctttttatc gcaactctct actgtttctc cataccgttt ttttgggcta gctactagag
     1261 aaagaggaga aatactagat gaaaaaatta ttattcgcaa ttcctttagt ggtacctttc
     1321 tattctcact ctactaatta taattatggg cctggtggag gttcggccga aactgttgaa
     1381 agttgtttag caaaatccca tacagaaaat tcatttacta acgtctggaa agacgacaaa
     1441 actttagatc gttacgctaa ctatgagggc tgtctgtgga atgctacagg cgttgtagtt
     1501 tgtactggtg acgaaactca gtgttacggt acatgggttc ctattgggct tgctatccct
     1561 gaaaatgagg gtggtggctc tgagggtggc ggttctgagg gtggcggttc tgagggtggc
     1621 ggtactaaac ctcctgagta cggtgataca cctattccgg gctatactta tatcaaccct
     1681 ctcgacggca cttatccgcc tggtactgag caaaaccccg ctaatcctaa tccttctctt
     1741 gaggagtctc agcctcttaa tactttcatg tttcagaata ataggttccg aaataggcag
     1801 ggggcattaa ctgtttatac gggcactgtt actcaaggca ctgaccccgt taaaacttat
     1861 taccagtaca ctcctgtatc atcaaaagcc atgtatgacg cttactggaa cggtaaattc
     1921 agagactgcg ctttccattc tggctttaat gaggatttat ttgtttgtga atatcaaggc
     1981 caatcgtctg acctgcctca acctcctgtc aatgctggcg gcggctctgg tggtggttct
     2041 ggtggcggct ctgagggtgg tggctctgag ggtggcggtt ctgagggtgg cggctctgag
     2101 ggaggcggtt ccggtggtgg ctctggttcc ggtgattttg attatgaaaa gatggcaaac
     2161 gctaataagg gggctatgac cgaaaatgcc gatgaaaacg cgctacagtc tgacgctaaa
     2221 ggcaaacttg attctgtcgc tactgattac ggtgctgcta tcgatggttt cattggtgac
     2281 gtttccggcc ttgctaatgg taatggtgct actggtgatt ttgctggctc taattcccaa
     2341 atggctcaag tcggtgacgg tgataattca cctttaatga ataatttccg tcaatattta
     2401 ccttccctcc ctcaatcggt tgaatgtcgc ccttttgtct ttggcgctgg taaaccatat
     2461 gaattttcta ttgattgtga caaaataaac ttattccgtg gtgtctttgc gtttctttta
     2521 tatgttgcca cctttatgta tgtattttct acgtttgcta acatactgcg taataaggag
     2581 tctaggtcat cagactacaa agatgacgac gataaataa
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