LOCUS       BBa_K1333313            1456 bp    DNA     linear       22-Dec-2024
DEFINITION  PIII with an RNA thermometer(PrfA)
ACCESSION   BBa_K1333313
VERSION     BBa_K1333313.1  
FEATURES             Location/Qualifiers
     RBS             106..109
                     /label=SD
     stem_loop       28..109
                     /label=Predicted stem loop up to A of AUG
     misc_feature    1..109
                     /label=BBa_K115003
     CDS             116..1423
                     /label=g3p with an 12 AAs peptide insertion designed for 
                     phage display
     CDS             1430..1456
                     /label=FLAG
     CDS             166..202
                     /label=12 AAs peptide insertion designed for phage display
     CDS             116..1456
                     /label=BBa_K1333002
ORIGIN
        1 tgtaaaaaat attatttagc gtgactttct agtaacagct aacaattgtt gttactgcct
       61 aatgttttta gggtatttta aaaaagggcg ataaaaaacg attgggggat actagatgaa
      121 aaaattatta ttcgcaattc ctttagtggt acctttctat tctcactcta ctaattataa
      181 ttatgggcct ggtggaggtt cggccgaaac tgttgaaagt tgtttagcaa aatcccatac
      241 agaaaattca tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta
      301 tgagggctgt ctgtggaatg ctacaggcgt tgtagtttgt actggtgacg aaactcagtg
      361 ttacggtaca tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga
      421 gggtggcggt tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg
      481 tgatacacct attccgggct atacttatat caaccctctc gacggcactt atccgcctgg
      541 tactgagcaa aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac
      601 tttcatgttt cagaataata ggttccgaaa taggcagggg gcattaactg tttatacggg
      661 cactgttact caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc
      721 aaaagccatg tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg
      781 ctttaatgag gatttatttg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc
      841 tcctgtcaat gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg
      901 ctctgagggt ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc
      961 tggttccggt gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga
     1021 aaatgccgat gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac
     1081 tgattacggt gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa
     1141 tggtgctact ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga
     1201 taattcacct ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga
     1261 atgtcgccct tttgtctttg gcgctggtaa accatatgaa ttttctattg attgtgacaa
     1321 aataaactta ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt
     1381 attttctacg tttgctaaca tactgcgtaa taaggagtct aggtcatcag actacaaaga
     1441 tgacgacgat aaataa
//