LOCUS       BBa_K1333314            2218 bp    DNA     linear       22-Nov-2024
DEFINITION  I13458-I13453-PrfA-RFP
ACCESSION   BBa_K1333314
VERSION     BBa_K1333314.1  
FEATURES             Location/Qualifiers
     misc_feature    1..1114
                     /label=BBa_I13458
     promoter        1123..1252
                     /label=BBa_I13453
     RBS             1366..1369
                     /label=SD
     stem_loop       1288..1369
                     /label=Predicted stem loop up to A of AUG
     misc_feature    1261..1369
                     /label=BBa_K115003
     CDS             1376..2050
                     /label=mrfp1
     misc_feature    2057..2081
                     /label=Help:Barcodes
     CDS             1376..2081
                     /label=BBa_E1010
     stem_loop       2101..2144
                     /label=stem_loop
     misc_feature    2090..2169
                     /label=BBa_B0010
     terminator      2090..2169
                     /label=BBa_B0010
     stem_loop       2185..2204
                     /label=T7 TE
     misc_feature    2211..2211
                     /label=stop
     polyA_site      2205..2218
                     /label=polya
     misc_feature    2178..2218
                     /label=BBa_B0012
     terminator      2178..2218
                     /label=BBa_B0012
     terminator      2090..2218
                     /label=BBa_B0015
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctattactag agacattgat tatttgcacg
     1141 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     1201 gctttttatc gcaactctct actgtttctc cataccgttt ttttgggcta gctactagag
     1261 tgtaaaaaat attatttagc gtgactttct agtaacagct aacaattgtt gttactgcct
     1321 aatgttttta gggtatttta aaaaagggcg ataaaaaacg attgggggat actagatggc
     1381 ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt
     1441 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca
     1501 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc
     1561 cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta
     1621 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg
     1681 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt
     1741 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg
     1801 ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa
     1861 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat
     1921 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac
     1981 ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc
     2041 caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa
     2101 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2161 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//