LOCUS       BBa_K1333316            1497 bp    DNA     linear       09-Jan-2025
DEFINITION  I13453-RBS-PⅢ(M13 phage)
ACCESSION   BBa_K1333316
VERSION     BBa_K1333316.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             157..1464
                     /label=g3p with an 12 AAs peptide insertion designed for 
                     phage display
     CDS             1471..1497
                     /label=FLAG
     CDS             207..243
                     /label=12 AAs peptide insertion designed for phage display
     CDS             157..1497
                     /label=BBa_K1333002
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga aaaaattatt attcgcaatt
      181 cctttagtgg tacctttcta ttctcactct actaattata attatgggcc tggtggaggt
      241 tcggccgaaa ctgttgaaag ttgtttagca aaatcccata cagaaaattc atttactaac
      301 gtctggaaag acgacaaaac tttagatcgt tacgctaact atgagggctg tctgtggaat
      361 gctacaggcg ttgtagtttg tactggtgac gaaactcagt gttacggtac atgggttcct
      421 attgggcttg ctatccctga aaatgagggt ggtggctctg agggtggcgg ttctgagggt
      481 ggcggttctg agggtggcgg tactaaacct cctgagtacg gtgatacacc tattccgggc
      541 tatacttata tcaaccctct cgacggcact tatccgcctg gtactgagca aaaccccgct
      601 aatcctaatc cttctcttga ggagtctcag cctcttaata ctttcatgtt tcagaataat
      661 aggttccgaa ataggcaggg ggcattaact gtttatacgg gcactgttac tcaaggcact
      721 gaccccgtta aaacttatta ccagtacact cctgtatcat caaaagccat gtatgacgct
      781 tactggaacg gtaaattcag agactgcgct ttccattctg gctttaatga ggatttattt
      841 gtttgtgaat atcaaggcca atcgtctgac ctgcctcaac ctcctgtcaa tgctggcggc
      901 ggctctggtg gtggttctgg tggcggctct gagggtggtg gctctgaggg tggcggttct
      961 gagggtggcg gctctgaggg aggcggttcc ggtggtggct ctggttccgg tgattttgat
     1021 tatgaaaaga tggcaaacgc taataagggg gctatgaccg aaaatgccga tgaaaacgcg
     1081 ctacagtctg acgctaaagg caaacttgat tctgtcgcta ctgattacgg tgctgctatc
     1141 gatggtttca ttggtgacgt ttccggcctt gctaatggta atggtgctac tggtgatttt
     1201 gctggctcta attcccaaat ggctcaagtc ggtgacggtg ataattcacc tttaatgaat
     1261 aatttccgtc aatatttacc ttccctccct caatcggttg aatgtcgccc ttttgtcttt
     1321 ggcgctggta aaccatatga attttctatt gattgtgaca aaataaactt attccgtggt
     1381 gtctttgcgt ttcttttata tgttgccacc tttatgtatg tattttctac gtttgctaac
     1441 atactgcgta ataaggagtc taggtcatca gactacaaag atgacgacga taaataa
//