LOCUS       BBa_K1333320            1399 bp    DNA     linear       23-Dec-2024
DEFINITION  FourU-PIII(M13)
ACCESSION   BBa_K1333320
VERSION     BBa_K1333320.1  
FEATURES             Location/Qualifiers
     RBS             47..52
                     /label=rbs
     misc_feature    23..28
                     /label=scar adaptation
     stem_loop       1..18
                     /label=stem_loop
     stem_loop       24..52
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    1..52
                     /label=BBa_K115002
     CDS             59..1366
                     /label=g3p with an 12 AAs peptide insertion designed for 
                     phage display
     CDS             1373..1399
                     /label=FLAG
     CDS             109..145
                     /label=12 AAs peptide insertion designed for phage display
     CDS             59..1399
                     /label=BBa_K1333002
ORIGIN
        1 ggacaagcaa tgcttgcctt gaatagtaac ttttgaatag tgattcagga ggtactagat
       61 gaaaaaatta ttattcgcaa ttcctttagt ggtacctttc tattctcact ctactaatta
      121 taattatggg cctggtggag gttcggccga aactgttgaa agttgtttag caaaatccca
      181 tacagaaaat tcatttacta acgtctggaa agacgacaaa actttagatc gttacgctaa
      241 ctatgagggc tgtctgtgga atgctacagg cgttgtagtt tgtactggtg acgaaactca
      301 gtgttacggt acatgggttc ctattgggct tgctatccct gaaaatgagg gtggtggctc
      361 tgagggtggc ggttctgagg gtggcggttc tgagggtggc ggtactaaac ctcctgagta
      421 cggtgataca cctattccgg gctatactta tatcaaccct ctcgacggca cttatccgcc
      481 tggtactgag caaaaccccg ctaatcctaa tccttctctt gaggagtctc agcctcttaa
      541 tactttcatg tttcagaata ataggttccg aaataggcag ggggcattaa ctgtttatac
      601 gggcactgtt actcaaggca ctgaccccgt taaaacttat taccagtaca ctcctgtatc
      661 atcaaaagcc atgtatgacg cttactggaa cggtaaattc agagactgcg ctttccattc
      721 tggctttaat gaggatttat ttgtttgtga atatcaaggc caatcgtctg acctgcctca
      781 acctcctgtc aatgctggcg gcggctctgg tggtggttct ggtggcggct ctgagggtgg
      841 tggctctgag ggtggcggtt ctgagggtgg cggctctgag ggaggcggtt ccggtggtgg
      901 ctctggttcc ggtgattttg attatgaaaa gatggcaaac gctaataagg gggctatgac
      961 cgaaaatgcc gatgaaaacg cgctacagtc tgacgctaaa ggcaaacttg attctgtcgc
     1021 tactgattac ggtgctgcta tcgatggttt cattggtgac gtttccggcc ttgctaatgg
     1081 taatggtgct actggtgatt ttgctggctc taattcccaa atggctcaag tcggtgacgg
     1141 tgataattca cctttaatga ataatttccg tcaatattta ccttccctcc ctcaatcggt
     1201 tgaatgtcgc ccttttgtct ttggcgctgg taaaccatat gaattttcta ttgattgtga
     1261 caaaataaac ttattccgtg gtgtctttgc gtttctttta tatgttgcca cctttatgta
     1321 tgtattttct acgtttgcta acatactgcg taataaggag tctaggtcat cagactacaa
     1381 agatgacgac gataaataa
//