LOCUS BBa_K1333321 2659 bp DNA linear 14-Nov-2024 DEFINITION I13458-I13453-FourU-PⅢ ACCESSION BBa_K1333321 VERSION BBa_K1333321.1 FEATURES Location/Qualifiers misc_feature 1..1114 /label=BBa_I13458 promoter 1123..1252 /label=BBa_I13453 RBS 1307..1312 /label=rbs misc_feature 1283..1288 /label=scar adaptation stem_loop 1261..1278 /label=stem_loop stem_loop 1284..1312 /label=predicted stem-loop extending to one position before start codon misc_feature 1261..1312 /label=BBa_K115002 CDS 1319..2626 /label=g3p with an 12 AAs peptide insertion designed for phage display CDS 2633..2659 /label=FLAG CDS 1369..1405 /label=12 AAs peptide insertion designed for phage display CDS 1319..2659 /label=BBa_K1333002 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctattactag agacattgat tatttgcacg 1141 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac 1201 gctttttatc gcaactctct actgtttctc cataccgttt ttttgggcta gctactagag 1261 ggacaagcaa tgcttgcctt gaatagtaac ttttgaatag tgattcagga ggtactagat 1321 gaaaaaatta ttattcgcaa ttcctttagt ggtacctttc tattctcact ctactaatta 1381 taattatggg cctggtggag gttcggccga aactgttgaa agttgtttag caaaatccca 1441 tacagaaaat tcatttacta acgtctggaa agacgacaaa actttagatc gttacgctaa 1501 ctatgagggc tgtctgtgga atgctacagg cgttgtagtt tgtactggtg acgaaactca 1561 gtgttacggt acatgggttc ctattgggct tgctatccct gaaaatgagg gtggtggctc 1621 tgagggtggc ggttctgagg gtggcggttc tgagggtggc ggtactaaac ctcctgagta 1681 cggtgataca cctattccgg gctatactta tatcaaccct ctcgacggca cttatccgcc 1741 tggtactgag caaaaccccg ctaatcctaa tccttctctt gaggagtctc agcctcttaa 1801 tactttcatg tttcagaata ataggttccg aaataggcag ggggcattaa ctgtttatac 1861 gggcactgtt actcaaggca ctgaccccgt taaaacttat taccagtaca ctcctgtatc 1921 atcaaaagcc atgtatgacg cttactggaa cggtaaattc agagactgcg ctttccattc 1981 tggctttaat gaggatttat ttgtttgtga atatcaaggc caatcgtctg acctgcctca 2041 acctcctgtc aatgctggcg gcggctctgg tggtggttct ggtggcggct ctgagggtgg 2101 tggctctgag ggtggcggtt ctgagggtgg cggctctgag ggaggcggtt ccggtggtgg 2161 ctctggttcc ggtgattttg attatgaaaa gatggcaaac gctaataagg gggctatgac 2221 cgaaaatgcc gatgaaaacg cgctacagtc tgacgctaaa ggcaaacttg attctgtcgc 2281 tactgattac ggtgctgcta tcgatggttt cattggtgac gtttccggcc ttgctaatgg 2341 taatggtgct actggtgatt ttgctggctc taattcccaa atggctcaag tcggtgacgg 2401 tgataattca cctttaatga ataatttccg tcaatattta ccttccctcc ctcaatcggt 2461 tgaatgtcgc ccttttgtct ttggcgctgg taaaccatat gaattttcta ttgattgtga 2521 caaaataaac ttattccgtg gtgtctttgc gtttctttta tatgttgcca cctttatgta 2581 tgtattttct acgtttgcta acatactgcg taataaggag tctaggtcat cagactacaa 2641 agatgacgac gataaataa //