LOCUS       BBa_K1334004            1431 bp    DNA     linear       29-Nov-2024
DEFINITION  P-formaldehyde+RBS+RFP+terminator
ACCESSION   BBa_K1334004
VERSION     BBa_K1334004.1  
FEATURES             Location/Qualifiers
     RBS             579..586
                     /label=rbs
     promoter        534..570
                     /label=Formaldehyde promoter
     promoter        422..470
                     /label=weak promoter
     misc_feature    514..538
                     /label=HxlR protein binding site 2
     misc_feature    489..513
                     /label=HxlR protein binding site 1
     CDS             1..363
                     /label=HxlR coding sequence
     promoter        1..595
                     /label=BBa_K1334002
     RBS             604..616
                     /label=RBS-3\Weak
     RBS             610..613
                     /label=RBS
     misc_feature    604..616
                     /label=BBa_B0032
     RBS             604..616
                     /label=BBa_B0032
     CDS             623..1297
                     /label=mrfp1
     misc_feature    1304..1328
                     /label=Help:Barcodes
     CDS             623..1328
                     /label=BBa_E1010
     stem_loop       1344..1363
                     /label=T7 TE
     misc_feature    1370..1370
                     /label=stop
     polyA_site      1364..1377
                     /label=polya
     misc_feature    1337..1377
                     /label=BBa_B0012
     terminator      1337..1377
                     /label=BBa_B0012
     stem_loop       1398..1420
                     /label=stem_loop
     misc_feature    1386..1431
                     /label=BBa_B0011
     terminator      1386..1431
                     /label=BBa_B0011
     terminator      1337..1431
                     /label=BBa_B0014
     misc_feature    604..1431
                     /label=BBa_K518012
ORIGIN
        1 ttacaacgat tctttcatga catttttgtc gatatcaatc aattccatat agcctttccc
       61 ccactcatac atggcttcaa gaataggcat gaggctttct ccgtgcgggg tcagagaata
      121 ttcaaccttc ggcgggacaa ctggatacac ttccctgtga acaatcatat cctgctcaag
      181 ctctctcagc tgattcacga ggatcttctg cgtaatatca ggaatcaatg tttttaattc
      241 attgaaccgt tttgtgcctt cttttcctaa atgccataaa atgagcattt tccatttacc
      301 gccaatcact gcaagcgtta attccttctc acaattaaac cttttgtcgt ccatccggct
      361 caatagaatc ccccccttag taaccttttt gtatgttaga tacgtatcat cacttactta
      421 cttttttgca gactcttttt tcattatact cactcataaa atcaagcgaa agaaatctga
      481 ttgtctctcc tcacagtatc ctccaagtaa cttgttgact tcaaagtgcc tacttctcaa
      541 caaaagaagg aacctataga atgaacaata attcgaaaaa gggagtggat caacatacta
      601 gagtcacaca ggaaagtact agatggcttc ctccgaagac gttatcaaag agttcatgcg
      661 tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg
      721 tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc
      781 gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt
      841 taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg
      901 ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct
      961 gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg
     1021 tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga
     1081 agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta
     1141 cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta
     1201 caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca
     1261 gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taacgctgat agtgctagtg
     1321 tagatcgcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac
     1381 tagagagaga atataaaaag ccagattatt aatccggctt ttttattatt t
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