BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939303
1
BBa_B0012
component939311
1
BBa_B0011
annotation939311
1
BBa_B0011
range939311
1
50
95
annotation939303
1
BBa_B0012
range939303
1
1
41
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_K1334004
1
BBa_K1334004
P-formaldehyde+RBS+RFP+terminator
2014-09-26T11:00:00Z
2015-05-08T01:09:58Z
The promoter comes from Bacillus subtilis 168 and the RBS+RFP+TERM comes from BBa_K518012.
The promoter is activited by formaldehyde and the downstream RFP will be expressed.
false
false
_1709_
0
22430
9
It's complicated
false
There may be leakage.
false
Haoyuan Sun
component2387335
1
BBa_K518012
component2387321
1
BBa_K1334002
annotation2387321
1
BBa_K1334002
range2387321
1
1
595
annotation2387335
1
BBa_K518012
range2387335
1
604
1431
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
BBa_K518012
1
BBa_K518012
RBS + RFP + d.Ter
2011-10-02T11:00:00Z
2015-05-08T01:12:33Z
From BioBrick parts.
Released HQ 2013
This part is an RFP coding device. When ligated with a promoter, it will yield a red colony so that you can determine whether your promoter works.
false
false
_683_
0
8305
9
In stock
false
None.
false
Masato Ohgishi
component2250776
1
BBa_B0032
component2250787
1
BBa_B0014
component2250780
1
BBa_E1010
annotation2250787
1
BBa_B0014
range2250787
1
734
828
annotation2250780
1
BBa_E1010
range2250780
1
20
725
annotation2250776
1
BBa_B0032
range2250776
1
1
13
BBa_K1334002
1
BBa_K1334002
HxlR+P-formaldehyde
2014-09-26T11:00:00Z
2015-05-08T01:09:58Z
We get this part from Bacillus subtilis 168.
A right promoter which can sense formaldehyde while there is a left promoter which can erpress the HxlR protein theoretically.The HxlR protein can enhance the function of the P-formaldehyde.
false
false
_1709_
0
22430
9
In stock
true
The start codon of the HxlR protein is UUG.
false
Haoyuan Sun
annotation2387313
1
HxlR coding sequence
range2387313
1
1
363
annotation2387265
1
Formaldehyde promoter
range2387265
1
534
570
annotation2387310
1
weak promoter
range2387310
1
422
470
annotation2387311
1
HxlR protein binding site 2
range2387311
1
514
538
annotation2387312
1
HxlR protein binding site 1
range2387312
1
489
513
annotation2387242
1
rbs
range2387242
1
579
586
BBa_E1010
1
mRFP1
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
2004-07-27T11:00:00Z
2015-08-31T04:07:26Z
Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a>
Released HQ 2013
monomeric RFP:
Red Fluorescent Protein.
Excitation peak: 584 nm
Emission peak: 607 nm
false
false
_11_1_
0
52
7
In stock
false
TAATAA double stop codon added (DE).
Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
true
Drew Endy
annotation1014044
1
mrfp1
range1014044
1
1
675
annotation2214014
1
Help:Barcodes
range2214014
1
682
706
BBa_K1334002_sequence
1
ttacaacgattctttcatgacatttttgtcgatatcaatcaattccatatagcctttcccccactcatacatggcttcaagaataggcatgaggctttctccgtgcggggtcagagaatattcaaccttcggcgggacaactggatacacttccctgtgaacaatcatatcctgctcaagctctctcagctgattcacgaggatcttctgcgtaatatcaggaatcaatgtttttaattcattgaaccgttttgtgccttcttttcctaaatgccataaaatgagcattttccatttaccgccaatcactgcaagcgttaattccttctcacaattaaaccttttgtcgtccatccggctcaatagaatcccccccttagtaacctttttgtatgttagatacgtatcatcacttacttacttttttgcagactcttttttcattatactcactcataaaatcaagcgaaagaaatctgattgtctctcctcacagtatcctccaagtaacttgttgacttcaaagtgcctacttctcaacaaaagaaggaacctatagaatgaacaataattcgaaaaagggagtggatcaaca
BBa_K518012_sequence
1
tcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_K1334004_sequence
1
ttacaacgattctttcatgacatttttgtcgatatcaatcaattccatatagcctttcccccactcatacatggcttcaagaataggcatgaggctttctccgtgcggggtcagagaatattcaaccttcggcgggacaactggatacacttccctgtgaacaatcatatcctgctcaagctctctcagctgattcacgaggatcttctgcgtaatatcaggaatcaatgtttttaattcattgaaccgttttgtgccttcttttcctaaatgccataaaatgagcattttccatttaccgccaatcactgcaagcgttaattccttctcacaattaaaccttttgtcgtccatccggctcaatagaatcccccccttagtaacctttttgtatgttagatacgtatcatcacttacttacttttttgcagactcttttttcattatactcactcataaaatcaagcgaaagaaatctgattgtctctcctcacagtatcctccaagtaacttgttgacttcaaagtgcctacttctcaacaaaagaaggaacctatagaatgaacaataattcgaaaaagggagtggatcaacatactagagtcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_E1010_sequence
1
atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
BBa_B0032_sequence
1
tcacacaggaaag
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z