BBa_K1334004
type: ComponentDefinition
persistent identity: BBa K1334004
display id: BBa_K1334004
version: 1
was generated by: iGEM to SBOL conversion
description: P-formaldehyde+RBS+RFP+terminator
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:09:58Z
mutable provenance: The promoter comes from Bacillus subtilis 168 and the RBS+RFP+TERM comes from BBa_K518012.
owned by: james
owned by: myers
mutable description: The promoter is activited by formaldehyde and the downstream RFP will be expressed.
discontinued: false
dominant: false
experience: experience/None
group u list: _1709_
m user id: 0
owner id: 22430
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: There may be leakage.
star: false
creator: Haoyuan Sun
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1334002
component: BBa_K518012
sequence annotation: BBa_K1334002
sequence annotation: BBa_K518012
sequence: https://synbiohub.org/public/igem/BBa_K1334004_sequence/1

BBa_K1334002
type: Component
persistent identity: component2387321
display id: component2387321
version: 1
top level: BBa_K1334004
definition: BBa_K1334002
access: public

BBa_K518012
type: Component
persistent identity: component2387335
display id: component2387335
version: 1
top level: BBa_K1334004
definition: BBa_K518012
access: public

BBa_K1334002
type: SequenceAnnotation
persistent identity: annotation2387321
display id: annotation2387321
version: 1
top level: BBa_K1334004
location: https://synbiohub.org/public/igem/BBa_K1334004/annotation2387321/range2387321/1
component: BBa_K1334002

https://synbiohub.org/public/igem/BBa_K1334004/annotation2387321/range2387321/1
type: Range
persistent identity: range2387321
display id: range2387321
version: 1
top level: BBa_K1334004
direction: direction/reverse
start: 1
end: 595
orientation: inline

BBa_K518012
type: SequenceAnnotation
persistent identity: annotation2387335
display id: annotation2387335
version: 1
top level: BBa_K1334004
location: https://synbiohub.org/public/igem/BBa_K1334004/annotation2387335/range2387335/1
component: BBa_K518012

https://synbiohub.org/public/igem/BBa_K1334004/annotation2387335/range2387335/1
type: Range
persistent identity: range2387335
display id: range2387335
version: 1
top level: BBa_K1334004
direction: direction/reverse
start: 604
end: 1431
orientation: inline

BBa_K518012
type: ComponentDefinition
persistent identity: BBa K518012
display id: BBa_K518012
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + RFP + d.Ter
created: 2011-10-02T11:00:00Z
modified: 2015-05-08T01:12:33Z
mutable provenance: From BioBrick parts.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part is an RFP coding device. When ligated with a promoter, it will yield a red colony so that you can determine whether your promoter works.
discontinued: false
dominant: false
experience: experience/Works
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None.
star: false
creator: Masato Ohgishi
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0014
component: BBa_E1010
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_E1010
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K518012_sequence/1

BBa_B0014
type: Component
persistent identity: component2250787
display id: component2250787
version: 1
top level: BBa_K518012
definition: BBa_B0014
access: public

BBa_E1010
type: Component
persistent identity: component2250780
display id: component2250780
version: 1
top level: BBa_K518012
definition: mRFP1
access: public

BBa_B0032
type: Component
persistent identity: component2250776
display id: component2250776
version: 1
top level: BBa_K518012
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2250776
display id: annotation2250776
version: 1
top level: BBa_K518012
location: https://synbiohub.org/public/igem/BBa_K518012/annotation2250776/range2250776/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K518012/annotation2250776/range2250776/1
type: Range
persistent identity: range2250776
display id: range2250776
version: 1
top level: BBa_K518012
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2250780
display id: annotation2250780
version: 1
top level: BBa_K518012
location: https://synbiohub.org/public/igem/BBa_K518012/annotation2250780/range2250780/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K518012/annotation2250780/range2250780/1
type: Range
persistent identity: range2250780
display id: range2250780
version: 1
top level: BBa_K518012
direction: direction/reverse
start: 20
end: 725
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2250787
display id: annotation2250787
version: 1
top level: BBa_K518012
location: https://synbiohub.org/public/igem/BBa_K518012/annotation2250787/range2250787/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K518012/annotation2250787/range2250787/1
type: Range
persistent identity: range2250787
display id: range2250787
version: 1
top level: BBa_K518012
direction: direction/reverse
start: 734
end: 828
orientation: inline

BBa_K1334002
type: ComponentDefinition
persistent identity: BBa K1334002
display id: BBa_K1334002
version: 1
was generated by: iGEM to SBOL conversion
description: HxlR+P-formaldehyde
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:09:58Z
mutable provenance: We get this part from Bacillus subtilis 168.
owned by: james
owned by: myers
mutable description: A right promoter which can sense formaldehyde while there is a left promoter which can erpress the HxlR protein theoretically.The HxlR protein can enhance the function of the P-formaldehyde.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1709_
m user id: 0
owner id: 22430
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The start codon of the HxlR protein is UUG.
star: false
creator: Haoyuan Sun
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: HxlR coding sequence
sequence annotation: weak promoter
sequence annotation: HxlR protein binding site 1
sequence annotation: HxlR protein binding site 2
sequence annotation: Formaldehyde promoter
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K1334002_sequence/1

HxlR coding sequence
type: SequenceAnnotation
persistent identity: annotation2387313
display id: annotation2387313
version: 1
top level: BBa_K1334002
location: https://synbiohub.org/public/igem/BBa_K1334002/annotation2387313/range2387313/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1334002/annotation2387313/range2387313/1
type: Range
persistent identity: range2387313
display id: range2387313
version: 1
top level: BBa_K1334002
direction: direction/reverse
start: 1
end: 363
orientation: inline

weak promoter
type: SequenceAnnotation
persistent identity: annotation2387310
display id: annotation2387310
version: 1
top level: BBa_K1334002
location: https://synbiohub.org/public/igem/BBa_K1334002/annotation2387310/range2387310/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1334002/annotation2387310/range2387310/1
type: Range
persistent identity: range2387310
display id: range2387310
version: 1
top level: BBa_K1334002
direction: direction/reverse
start: 422
end: 470
orientation: inline

HxlR protein binding site 1
type: SequenceAnnotation
persistent identity: annotation2387312
display id: annotation2387312
version: 1
top level: BBa_K1334002
location: https://synbiohub.org/public/igem/BBa_K1334002/annotation2387312/range2387312/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K1334002/annotation2387312/range2387312/1
type: Range
persistent identity: range2387312
display id: range2387312
version: 1
top level: BBa_K1334002
direction: direction/forward
start: 489
end: 513
orientation: inline

HxlR protein binding site 2
type: SequenceAnnotation
persistent identity: annotation2387311
display id: annotation2387311
version: 1
top level: BBa_K1334002
location: https://synbiohub.org/public/igem/BBa_K1334002/annotation2387311/range2387311/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1334002/annotation2387311/range2387311/1
type: Range
persistent identity: range2387311
display id: range2387311
version: 1
top level: BBa_K1334002
direction: direction/forward
start: 514
end: 538
orientation: inline

Formaldehyde promoter
type: SequenceAnnotation
persistent identity: annotation2387265
display id: annotation2387265
version: 1
top level: BBa_K1334002
location: https://synbiohub.org/public/igem/BBa_K1334002/annotation2387265/range2387265/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1334002/annotation2387265/range2387265/1
type: Range
persistent identity: range2387265
display id: range2387265
version: 1
top level: BBa_K1334002
direction: direction/forward
start: 534
end: 570
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2387242
display id: annotation2387242
version: 1
top level: BBa_K1334002
location: https://synbiohub.org/public/igem/BBa_K1334002/annotation2387242/range2387242/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1334002/annotation2387242/range2387242/1
type: Range
persistent identity: range2387242
display id: range2387242
version: 1
top level: BBa_K1334002
direction: direction/forward
start: 579
end: 586
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_K1334004_sequence/1
type: Sequence
persistent identity: BBa K1334004 sequence
display id: BBa_K1334004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttacaacgattctttcatgacatttttgtcgatatcaatcaattccatatagcctttcccccactcatacatggcttcaagaataggcatgaggctttctccgtgcggggtcagagaatattcaaccttcggcgggacaactggatacacttccctgtgaacaatcatatcctgctcaagctctctcagctgattcacgaggatcttctgcgtaatatcaggaatcaatgtttttaattcattgaaccgttttgtgccttcttttcctaaatgccataaaatgagcattttccatttaccgccaatcactgcaagcgttaattccttctcacaattaaaccttttgtcgtccatccggctcaatagaatcccccccttagtaacctttttgtatgttagatacgtatcatcacttacttacttttttgcagactcttttttcattatactcactcataaaatcaagcgaaagaaatctgattgtctctcctcacagtatcctccaagtaacttgttgacttcaaagtgcctacttctcaacaaaagaaggaacctatagaatgaacaataattcgaaaaagggagtggatcaacatactagagtcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K518012_sequence/1
type: Sequence
persistent identity: BBa K518012 sequence
display id: BBa_K518012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1334002_sequence/1
type: Sequence
persistent identity: BBa K1334002 sequence
display id: BBa_K1334002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttacaacgattctttcatgacatttttgtcgatatcaatcaattccatatagcctttcccccactcatacatggcttcaagaataggcatgaggctttctccgtgcggggtcagagaatattcaaccttcggcgggacaactggatacacttccctgtgaacaatcatatcctgctcaagctctctcagctgattcacgaggatcttctgcgtaatatcaggaatcaatgtttttaattcattgaaccgttttgtgccttcttttcctaaatgccataaaatgagcattttccatttaccgccaatcactgcaagcgttaattccttctcacaattaaaccttttgtcgtccatccggctcaatagaatcccccccttagtaacctttttgtatgttagatacgtatcatcacttacttacttttttgcagactcttttttcattatactcactcataaaatcaagcgaaagaaatctgattgtctctcctcacagtatcctccaagtaacttgttgacttcaaagtgcctacttctcaacaaaagaaggaacctatagaatgaacaataattcgaaaaagggagtggatcaaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z