LOCUS       BBa_K1334005            1478 bp    DNA     linear       22-Dec-2024
DEFINITION  P-formaldehyde+RBS+GFP+terminator
ACCESSION   BBa_K1334005
VERSION     BBa_K1334005.1  
FEATURES             Location/Qualifiers
     RBS             579..586
                     /label=rbs
     promoter        534..570
                     /label=Formaldehyde promoter
     promoter        422..470
                     /label=weak promoter
     misc_feature    514..538
                     /label=HxlR protein binding site 2
     misc_feature    489..513
                     /label=HxlR protein binding site 1
     CDS             1..363
                     /label=HxlR coding sequence
     promoter        1..595
                     /label=BBa_K1334002
     misc_feature    608..611
                     /label=conserved
     RBS             604..615
                     /label=BBa_B0034
     CDS             622..1341
                     /label=GFP
     misc_feature    622..1341
                     /label=BBa_K895006
     stem_loop       1361..1404
                     /label=stem_loop
     misc_feature    1350..1429
                     /label=BBa_B0010
     terminator      1350..1429
                     /label=BBa_B0010
     stem_loop       1445..1464
                     /label=T7 TE
     misc_feature    1471..1471
                     /label=stop
     polyA_site      1465..1478
                     /label=polya
     misc_feature    1438..1478
                     /label=BBa_B0012
     terminator      1438..1478
                     /label=BBa_B0012
     terminator      1350..1478
                     /label=BBa_B0015
ORIGIN
        1 ttacaacgat tctttcatga catttttgtc gatatcaatc aattccatat agcctttccc
       61 ccactcatac atggcttcaa gaataggcat gaggctttct ccgtgcgggg tcagagaata
      121 ttcaaccttc ggcgggacaa ctggatacac ttccctgtga acaatcatat cctgctcaag
      181 ctctctcagc tgattcacga ggatcttctg cgtaatatca ggaatcaatg tttttaattc
      241 attgaaccgt tttgtgcctt cttttcctaa atgccataaa atgagcattt tccatttacc
      301 gccaatcact gcaagcgtta attccttctc acaattaaac cttttgtcgt ccatccggct
      361 caatagaatc ccccccttag taaccttttt gtatgttaga tacgtatcat cacttactta
      421 cttttttgca gactcttttt tcattatact cactcataaa atcaagcgaa agaaatctga
      481 ttgtctctcc tcacagtatc ctccaagtaa cttgttgact tcaaagtgcc tacttctcaa
      541 caaaagaagg aacctataga atgaacaata attcgaaaaa gggagtggat caacatacta
      601 gagaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
      661 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      721 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      781 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      841 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      901 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      961 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
     1021 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
     1081 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
     1141 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
     1201 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
     1261 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     1321 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc
     1381 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1441 cactggctca ccttcgggtg ggcctttctg cgtttata
//