BBa_K1334006 1 BBa_K1334006 P-formaldehyde+RBS+RFP 2014-09-26T11:00:00Z 2015-05-08T01:09:58Z The promoter comes from Bacillus subtilis 168 and the RFP comes from BBa_E1010. The promoter is activited by formaldehyde and the downstream RFP will be expressed. false false _1709_ 0 22430 9 In stock false There may be leakage. false Haoyuan Sun component2387360 1 BBa_K1334002 component2387363 1 BBa_E1010 annotation2387360 1 BBa_K1334002 range2387360 1 1 595 annotation2387363 1 BBa_E1010 range2387363 1 602 1307 BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation1014044 1 mrfp1 range1014044 1 1 675 annotation2214014 1 Help:Barcodes range2214014 1 682 706 BBa_K1334002 1 BBa_K1334002 HxlR+P-formaldehyde 2014-09-26T11:00:00Z 2015-05-08T01:09:58Z We get this part from Bacillus subtilis 168. A right promoter which can sense formaldehyde while there is a left promoter which can erpress the HxlR protein theoretically.The HxlR protein can enhance the function of the P-formaldehyde. false false _1709_ 0 22430 9 In stock true The start codon of the HxlR protein is UUG. false Haoyuan Sun annotation2387313 1 HxlR coding sequence range2387313 1 1 363 annotation2387311 1 HxlR protein binding site 2 range2387311 1 514 538 annotation2387265 1 Formaldehyde promoter range2387265 1 534 570 annotation2387242 1 rbs range2387242 1 579 586 annotation2387310 1 weak promoter range2387310 1 422 470 annotation2387312 1 HxlR protein binding site 1 range2387312 1 489 513 BBa_K1334002_sequence 1 ttacaacgattctttcatgacatttttgtcgatatcaatcaattccatatagcctttcccccactcatacatggcttcaagaataggcatgaggctttctccgtgcggggtcagagaatattcaaccttcggcgggacaactggatacacttccctgtgaacaatcatatcctgctcaagctctctcagctgattcacgaggatcttctgcgtaatatcaggaatcaatgtttttaattcattgaaccgttttgtgccttcttttcctaaatgccataaaatgagcattttccatttaccgccaatcactgcaagcgttaattccttctcacaattaaaccttttgtcgtccatccggctcaatagaatcccccccttagtaacctttttgtatgttagatacgtatcatcacttacttacttttttgcagactcttttttcattatactcactcataaaatcaagcgaaagaaatctgattgtctctcctcacagtatcctccaagtaacttgttgacttcaaagtgcctacttctcaacaaaagaaggaacctatagaatgaacaataattcgaaaaagggagtggatcaaca BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_K1334006_sequence 1 ttacaacgattctttcatgacatttttgtcgatatcaatcaattccatatagcctttcccccactcatacatggcttcaagaataggcatgaggctttctccgtgcggggtcagagaatattcaaccttcggcgggacaactggatacacttccctgtgaacaatcatatcctgctcaagctctctcagctgattcacgaggatcttctgcgtaatatcaggaatcaatgtttttaattcattgaaccgttttgtgccttcttttcctaaatgccataaaatgagcattttccatttaccgccaatcactgcaagcgttaattccttctcacaattaaaccttttgtcgtccatccggctcaatagaatcccccccttagtaacctttttgtatgttagatacgtatcatcacttacttacttttttgcagactcttttttcattatactcactcataaaatcaagcgaaagaaatctgattgtctctcctcacagtatcctccaagtaacttgttgacttcaaagtgcctacttctcaacaaaagaaggaacctatagaatgaacaataattcgaaaaagggagtggatcaacatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z