LOCUS       BBa_K1334007            1101 bp    DNA     linear       06-Dec-2025
DEFINITION  K1334000+RBS+RFP+Terminator
ACCESSION   BBa_K1334007
VERSION     BBa_K1334007.1  
FEATURES             Location/Qualifiers
     RBS             215..222
                     /label=rbs
     promoter        171..207
                     /label=Formaldehyde promoter
     misc_feature    126..150
                     /label=HxlR protein binding site 1
     misc_feature    151..175
                     /label=HxlR protein binding site 2
     promoter        73..107
                     /label=A theoretically promoter(very weak)
     promoter        1..231
                     /label=BBa_K1334000
     RBS             240..252
                     /label=RBS-3\Weak
     RBS             246..249
                     /label=RBS
     misc_feature    240..252
                     /label=BBa_B0032
     RBS             240..252
                     /label=BBa_B0032
     CDS             259..933
                     /label=mrfp1
     misc_feature    940..964
                     /label=Help:Barcodes
     CDS             259..964
                     /label=BBa_E1010
     stem_loop       984..1027
                     /label=stem_loop
     misc_feature    973..1052
                     /label=BBa_B0010
     terminator      973..1052
                     /label=BBa_B0010
     stem_loop       1068..1087
                     /label=T7 TE
     misc_feature    1094..1094
                     /label=stop
     polyA_site      1088..1101
                     /label=polya
     misc_feature    1061..1101
                     /label=BBa_B0012
     terminator      1061..1101
                     /label=BBa_B0012
     terminator      973..1101
                     /label=BBa_B0015
ORIGIN
        1 tagaatcccc cccttagtaa cctttttgta tgttagatac gtatcatcac ttacttactt
       61 ttttgcagac tcttttttca ttatactcac tcataaaatc aagcgaaaga aatctgattg
      121 tctctcctca cagtatcctc caagtaactt gttgacttca aagtgcctac ttctcaacaa
      181 aagaaggaac ctatagaatg aacaataatt cgaaaaaggg agtggatcaa ctactagagt
      241 cacacaggaa agtactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc
      301 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa
      361 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg
      421 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa
      481 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa
      541 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa
      601 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg
      661 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac
      721 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac
      781 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa
      841 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac
      901 gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga
      961 tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1021 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     1081 gtgggccttt ctgcgtttat a
//