BBa_K1334017
1
BBa_K1334017
PADH from Pseudomonas putida
2014-09-26T11:00:00Z
2015-05-08T01:09:58Z
Using the genomic DNA of P. putida as the template, the coding regions of the PADH gene (padh) was amplified by PCR. The PCR products were then subcloned into PSB1C3
PADH from Pseudomonas putida is a formaldehyde dehydrogenase which can oxidize free formaldehyde to formate independent of GSH.This part is the coding sequence for the enzyme PADH
false
false
_1709_
0
22676
9
It's complicated
false
The original sequence contains a Not1 site,by point mutation,we removed the site
false
Qiushuang Wu
BBa_K1334017_sequence
1
atgtctggcaatcgtggagtggtatatctcggcgccggcaaggtcgaagtgcaacacatcgactacccgaaaatgcaggacccgcgtggcaagaagatcgagcacggcgtcatcctgaaggtggtctccaccaacatctgcggctctgaccagcacatggtccgtggccgcaccactgcccaggtcggcctggttctgggccacgaaatcaccggtgaaattgtcgagatcgggcgtgacgtcgaacgcttgaaaatcggtgacctggtgtcggtaccgttcaacgttgcctgtggccgctgccgctcctgcaaagagatgcacaccggcgtctgcctcaccgtcaacccggcccgcgccggtggtgcctacggctacgtcgacatgggcgactggaccggcggccaggctgagtacgtgctggtgccatacgccgacttcaacctgctgaaactgccggaccgcgacaaggccatggaaaagatccgtgacctgacatgcctgtccgacattctgcctaccggctaccacggtgccgtgacggctggcgtaggcccaggcagcaccgtttacgttgcgggcgctggcccggtcggcctggccgctgctgcctcggcccgcctgctgggcgcggcctgcgtcatcgtcggtgacctgaacccggcccgcctggcccacgccaagtcgcaaggctttgaagtggtcgacctgtccaaggacaccccgctgcacgagcagatcatcgacatcctcggtgagccggaagtggactgcgccgtcgacgccgtcggcttcgaggcccgtggccatggtcacgaaggtgccaagcacgaggccccggccaccgtgctgaactcgctgatgcaagtgacccgcgtggccggcaacatcggtatcccgggcctgtacgtaaccgaagacccaggcgcggtagatgccgctgccaagatcggcgcgctgagcatccgcttcggcctgggctgggcgaagtcgcacagcttccacaccggccagaccccgaccatgaagtacaaccgccagctgatgcaggcgatcatgtgggaccgtatcaacattgctgaagtggtgggggtgcaggtgatcaacctggatcaggcgccagaagggtatggcgagtttgatgcgggtgtaccgaagaagtttgtgattgacccgcacaagatgtggggcgcggcgtgaggcgcggcgtga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z