BBa_K1334017 1 BBa_K1334017 PADH from Pseudomonas putida 2014-09-26T11:00:00Z 2015-05-08T01:09:58Z Using the genomic DNA of P. putida as the template, the coding regions of the PADH gene (padh) was amplified by PCR. The PCR products were then subcloned into PSB1C3 PADH from Pseudomonas putida is a formaldehyde dehydrogenase which can oxidize free formaldehyde to formate independent of GSH.This part is the coding sequence for the enzyme PADH false false _1709_ 0 22676 9 It's complicated false The original sequence contains a Not1 site,by point mutation,we removed the site false Qiushuang Wu BBa_K1334017_sequence 1 atgtctggcaatcgtggagtggtatatctcggcgccggcaaggtcgaagtgcaacacatcgactacccgaaaatgcaggacccgcgtggcaagaagatcgagcacggcgtcatcctgaaggtggtctccaccaacatctgcggctctgaccagcacatggtccgtggccgcaccactgcccaggtcggcctggttctgggccacgaaatcaccggtgaaattgtcgagatcgggcgtgacgtcgaacgcttgaaaatcggtgacctggtgtcggtaccgttcaacgttgcctgtggccgctgccgctcctgcaaagagatgcacaccggcgtctgcctcaccgtcaacccggcccgcgccggtggtgcctacggctacgtcgacatgggcgactggaccggcggccaggctgagtacgtgctggtgccatacgccgacttcaacctgctgaaactgccggaccgcgacaaggccatggaaaagatccgtgacctgacatgcctgtccgacattctgcctaccggctaccacggtgccgtgacggctggcgtaggcccaggcagcaccgtttacgttgcgggcgctggcccggtcggcctggccgctgctgcctcggcccgcctgctgggcgcggcctgcgtcatcgtcggtgacctgaacccggcccgcctggcccacgccaagtcgcaaggctttgaagtggtcgacctgtccaaggacaccccgctgcacgagcagatcatcgacatcctcggtgagccggaagtggactgcgccgtcgacgccgtcggcttcgaggcccgtggccatggtcacgaaggtgccaagcacgaggccccggccaccgtgctgaactcgctgatgcaagtgacccgcgtggccggcaacatcggtatcccgggcctgtacgtaaccgaagacccaggcgcggtagatgccgctgccaagatcggcgcgctgagcatccgcttcggcctgggctgggcgaagtcgcacagcttccacaccggccagaccccgaccatgaagtacaaccgccagctgatgcaggcgatcatgtgggaccgtatcaacattgctgaagtggtgggggtgcaggtgatcaacctggatcaggcgccagaagggtatggcgagtttgatgcgggtgtaccgaagaagtttgtgattgacccgcacaagatgtggggcgcggcgtgaggcgcggcgtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z