LOCUS       BBa_K1334019            2399 bp    DNA     linear       26-Dec-2024
DEFINITION  promoter+RBS+FDH+terminator
ACCESSION   BBa_K1334019
VERSION     BBa_K1334019.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23102
     RBS             44..55
                     /label=BBa_J61100
     CDS             62..2262
                     /label=protein
     misc_feature    2236..2259
                     /label=Flag-tag
     CDS             62..2262
                     /label=BBa_K1334014
     stem_loop       2282..2325
                     /label=stem_loop
     misc_feature    2271..2350
                     /label=BBa_B0010
     terminator      2271..2350
                     /label=BBa_B0010
     stem_loop       2366..2385
                     /label=T7 TE
     misc_feature    2392..2392
                     /label=stop
     polyA_site      2386..2399
                     /label=polya
     misc_feature    2359..2399
                     /label=BBa_B0012
     terminator      2359..2399
                     /label=BBa_B0012
     terminator      2271..2399
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gagaaagagg ggacatacta
       61 gatgaaaaaa gtcgtcacgg tttgccccta ttgcgcatca ggttgcaaaa tcaacctggt
      121 cgtcgataac ggcaaaatcg tccgggcgga ggcagcgcag gggaaaacca accagggtac
      181 cctgtgtctg aagggttatt atggctggga cttcattaac gatacccaga tcctgacccc
      241 gcgcctgaaa acccccatga tccgtcgcca gcgtggcggc aaactcgaac ctgtttcctg
      301 ggatgaggca ctgaattacg ttgccgagcg cctgagcgcc atcaaagaga agtacggtcc
      361 ggatgccatc cagacgaccg gctcctcgcg tggtacgggt aacgaaacca actatgtaat
      421 gcaaaaattt gcgcgcgccg ttattggtac caataacgtt gactgctgcg ctcgtgtctg
      481 acacggccca tcggttgcag gtctgcacca atcggtcggt aatggcgcaa tgagcaatgc
      541 tattaacgaa attgataata ccgatttagt gttcgttttc gggtacaacc cggcggattc
      601 ccacccaatc gtggcgaatc acgtaattaa cgctaaacgt aacggggcga aaattatcgt
      661 ctgcgatccg cgcaaaattg aaaccgcgcg cattgctgac atgcacattg cactgaaaaa
      721 cggctcgaac atcgcgctgt tgaatgcgat gggccatgtc attattgaag aaaatctgta
      781 cgacaaagcg ttcgtcgctt cacgtacaga aggctttgaa gagtatcgta aaatcgttga
      841 aggctacacg ccggagtcgg ttgaagatat caccggcgtc agcgccagtg agattcgtca
      901 ggcggcacgg atgtatgccc aggcgaaaag cgccgccatc ctgtggggca tgggtgtaac
      961 ccagttctac cagggcgtgg aaaccgtgcg ttctctgacc agcctcgcga tgctgaccgg
     1021 taacctcggt aagccgcatg cgggtgttaa cccggttcgt ggtcagaaca acgttcaggg
     1081 tgcctgcgat atgggcgcgc tgccggatac gtatccggga taccagtacg tgaaagatcc
     1141 ggctaaccgc gagaaattcg ccaaagcctg gggcgtggaa agcctgccag cgcataccgg
     1201 ctatcgcatc agcgagctgc cgcaccgcgc agcgcatggc gaagtgcgtg ccgcgtacat
     1261 tatgggcgaa gatccgctac aaactgacgc ggagctgtcg gcagtacgta aagcctttga
     1321 agatctggaa ctggttatcg ttcaggacat ctttatgacc aaaaccgcgt cggcggcgga
     1381 tgttatttta ccgtcaacgt cgtggggcga gcatgaaggc gtgtttactg cggctgaccg
     1441 tggcttccag cgtttcttca aggcggttga accgaaatgg gatctgaaaa cggactggca
     1501 aatcatcagt gaaatcgcca cccgtatggg ttatccgatg cactacaaca acacccagga
     1561 gatctgggat gagttgcgtc atctgtgccc ggatttctac ggtgcgactt acgagaaaat
     1621 gggcgaactg ggcttcattc agtggccttg ccgcgatact tcagatgccg atcaggggac
     1681 ttcttatctg tttaaagaga agtttgatac cccgaacggt ctggcgcagt tcttcacctg
     1741 cgactgggta gcgccaatcg acaaactcac cgacgagtac ccgatggtac tgtcaacggt
     1801 gcgtgaagtt ggtcactact cttgccgttc gatgaccggt aactgtgcgg cactggcggc
     1861 gctggctgat gaacctggct acgcacaaat caataccgaa gacgccaaac gtctgggtat
     1921 tgaagatgag gcattggttt gggtgcactc gcgtaaaggc aaaattatca cccgtgcgca
     1981 ggtcagcgat cgtccgaaca aaggggcgat ttacatgacc taccagtggt ggattggtgc
     2041 ctgtaacgag ctggttaccg aaaacttaag cccgattacg aaaacgccgg agtacaaata
     2101 ctgcgccgtt cgcgtcgagc cgatcgccga tcagcgcgcc gccgagcagt acgtgattga
     2161 cgagtacaac aagttgaaaa ctcgcctgcg cgaagcggca ctggcgtaac tcgcctgcgc
     2221 gaagcggcac tggcggacta caaggacgac gatgacaagt aatactagag ccaggcatca
     2281 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     2341 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//