LOCUS       BBa_K1334020            1940 bp    DNA     linear       20-Sep-2024
DEFINITION  a signal complex to test whether the lux system works
ACCESSION   BBa_K1334020
VERSION     BBa_K1334020.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     misc_feature    82..83
                     /label=prefix
     misc_feature    255..255
                     /label=T
     misc_feature    573..573
                     /label=A
     CDS             82..831
                     /label=luxR
     misc_feature    82..837
                     /label=BBa_C0062
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             82..837
                     /label=BBa_C0062
     misc_feature    63..862
                     /label=BBa_K116616
     stem_loop       882..925
                     /label=stem_loop
     misc_feature    871..950
                     /label=BBa_B0010
     terminator      871..950
                     /label=BBa_B0010
     stem_loop       966..985
                     /label=T7 TE
     misc_feature    992..992
                     /label=stop
     polyA_site      986..999
                     /label=polya
     misc_feature    959..999
                     /label=BBa_B0012
     terminator      959..999
                     /label=BBa_B0012
     terminator      871..999
                     /label=BBa_B0015
     misc_feature    63..999
                     /label=BBa_K116626
     misc_feature    1..999
                     /label=BBa_K116640
     misc_feature    1008..1104
                     /label=BBa_R0065
     misc_feature    1013..1032
                     /label=Lux Box
     promoter        1054..1059
                     /label=-10
     misc_feature    1064..1080
                     /label=OR2 cI
     promoter        1065..1065
                     /label=lux p(R) start
     promoter        1078..1083
                     /label=-35
     misc_feature    1088..1104
                     /label=OR1 cI
     promoter        1101..1104
                     /label=-10
     promoter        1008..1104
                     /label=BBa_R0065
     RBS             1113..1125
                     /label=RBS-3\Weak
     RBS             1119..1122
                     /label=RBS
     misc_feature    1113..1125
                     /label=BBa_B0032
     RBS             1113..1125
                     /label=BBa_B0032
     CDS             1132..1806
                     /label=mrfp1
     misc_feature    1813..1837
                     /label=Help:Barcodes
     CDS             1132..1837
                     /label=BBa_E1010
     stem_loop       1853..1872
                     /label=T7 TE
     misc_feature    1879..1879
                     /label=stop
     polyA_site      1873..1886
                     /label=polya
     misc_feature    1846..1886
                     /label=BBa_B0012
     terminator      1846..1886
                     /label=BBa_B0012
     stem_loop       1907..1929
                     /label=stem_loop
     misc_feature    1895..1940
                     /label=BBa_B0011
     terminator      1895..1940
                     /label=BBa_B0011
     terminator      1846..1940
                     /label=BBa_B0014
     misc_feature    1113..1940
                     /label=BBa_K518012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gatgaaaaac ataaatgccg acgacacata cagaataatt
      121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact
      181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa
      241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct
      301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat
      361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg
      421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc
      481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat
      541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat
      601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg
      661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact
      721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt
      781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac
      841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      961 acactggctc accttcgggt gggcctttct gcgtttatat actagagtaa gcacctgtag
     1021 gatcgtacag gtttacgcaa gaaaatggtt tgttatagtc gaataacacc gtgcgtgttg
     1081 actattttac ctctggcggt gatatactag agtcacacag gaaagtacta gatggcttcc
     1141 tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac
     1201 ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc
     1261 gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg
     1321 cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg
     1381 aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt
     1441 gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa
     1501 ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg
     1561 gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg
     1621 cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct
     1681 aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc
     1741 cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc
     1801 ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagtcaca ctggctcacc
     1861 ttcgggtggg cctttctgcg tttatatact agagagagaa tataaaaagc cagattatta
     1921 atccggcttt tttattattt
//