##gff-version 3
##sequence-region BBa_K1334020 1 1940
BBa_K1334020	.	promoter	1008	1104	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_K1334020	.	promoter	1078	1083	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_K1334020	.	promoter	1065	1065	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_K1334020	.	non_covalent_binding_site	1013	1032	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_K1334020	.	engineered_region	1008	1104	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_K1334020	.	promoter	1101	1104	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_K1334020	.	non_covalent_binding_site	1088	1104	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_K1334020	.	promoter	1054	1059	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_K1334020	.	non_covalent_binding_site	1064	1080	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_K1334020	.	engineered_region	1113	1940	.	+	0	ID=BBa_K518012;Name=BBa_K518012
BBa_K1334020	.	ribosome_entry_site	1113	1125	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K518012
BBa_K1334020	.	engineered_region	1113	1125	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1334020	.	ribosome_entry_site	1113	1125	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1334020	.	ribosome_entry_site	1119	1122	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1334020	.	terminator	1846	1940	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K518012
BBa_K1334020	.	terminator	1846	1886	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K1334020	.	polyA_site	1873	1886	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1334020	.	stop_codon	1879	1879	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1334020	.	stem_loop	1853	1872	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1334020	.	engineered_region	1846	1886	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1334020	.	terminator	1895	1940	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K1334020	.	stem_loop	1907	1929	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K1334020	.	engineered_region	1895	1940	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K1334020	.	CDS	1132	1837	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K518012
BBa_K1334020	.	CDS	1132	1806	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1334020	.	engineered_tag	1813	1837	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1334020	.	engineered_region	1	999	.	+	0	ID=BBa_K116640;Name=BBa_K116640
BBa_K1334020	.	engineered_region	63	999	.	+	0	ID=BBa_K116626;Name=BBa_K116626;Parent=BBa_K116640
BBa_K1334020	.	engineered_region	63	862	.	+	0	ID=BBa_K116616;Name=BBa_K116616;Parent=BBa_K116626
BBa_K1334020	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116616
BBa_K1334020	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1334020	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1334020	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1334020	.	CDS	82	837	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K116616
BBa_K1334020	.	point_mutation	573	573	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1334020	.	engineered_tag	838	862	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1334020	.	point_mutation	255	255	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1334020	.	engineered_region	82	837	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1334020	.	engineered_region	82	83	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1334020	.	CDS	82	831	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1334020	.	terminator	871	999	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116626
BBa_K1334020	.	terminator	959	999	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1334020	.	polyA_site	986	999	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1334020	.	stop_codon	992	992	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1334020	.	stem_loop	966	985	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1334020	.	engineered_region	959	999	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1334020	.	terminator	871	950	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1334020	.	stem_loop	882	925	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1334020	.	engineered_region	871	950	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1334020	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K116640
BBa_K1334020	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1334020	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1334020	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1334020	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1334020	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_K1334020
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtact
agatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaa
tcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtt
aaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatg
atcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaa
atctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaat
ggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaaca
taccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaac
caaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagt
gagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaag
caattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaagcacctgtaggatcgta
caggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgatata
ctagagtcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatg
gaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgcta
aactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagc
ttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatg
aacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaac
tgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtat
gtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaa
gttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacct
cccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctg
atagtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagag
agaatataaaaagccagattattaatccggcttttttattattt